Cell freezing medium for human neural stem cells
A cryopreservation solution and stem cell technology, applied in the field of cell cryopreservation solution, can solve the problems of inability to guarantee cell viability, high requirement of cell protection solution, increase of animal pathogen pollution, etc. High survival rate and excellent freezing effect
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Embodiment 1
[0030] Example 1 Preparation of cell cryopreservation solution for human neural stem cells
[0031] It is composed of cryopreservation solution A and cryopreservation solution B, wherein 1L of cryopreservation solution A is composed of the following components: 20% albumin, 6.0g / L glucose, 0.6mg / L vitamin B12, 0.365mg / L Glutamine, 7.5g / L sodium chloride, 0.025g / L magnesium chloride, 0.3g / L potassium chloride, and the balance is water.
[0032] 1L of freezing solution B is composed of the following components: 15% dimethyl sulfoxide, 6.0g / L glucose, 0.6mg / L vitamin B12, 0.365mg / L glutamine, 7.5g / L sodium chloride , 0.025g / L magnesium chloride, 0.3g / L potassium chloride, and the balance is water.
[0033] The preparation method of cell cryopreservation solution is as follows: prepare cryopreservation solution A and cryopreservation solution B respectively; add each component into ultrapure water, mix and dissolve, and shake and mix with an oscillator for 2 to 3 minutes during t...
Embodiment 2
[0034] Example 2 Preparation of cell cryopreservation solution for human neural stem cells
[0035] It consists of cryopreservation solution A and cryopreservation solution B, wherein, cryopreservation solution A is composed of the following components: 10% albumin, 8.0g / L glucose, 0.4mg / L vitamin B12, 0.365mg / L glutamine Amide, 9.0g / L sodium chloride, 0.015g / L magnesium chloride, 0.4g / L potassium chloride, and the balance is water.
[0036] Freezing solution B is composed of the following components: 10% dimethyl sulfoxide, 8.0g / L glucose, 0.4mg / L vitamin B12, 0.365mg / L glutamine, 9.0g / L sodium chloride, 0.015 g / L magnesium chloride, 0.4g / L potassium chloride, and the balance is water.
[0037] The preparation method of cell cryopreservation solution is as follows: prepare cryopreservation solution A and cryopreservation solution B respectively; add each component into ultrapure water, mix and dissolve, and shake and mix with an oscillator for 2 to 3 minutes during the disso...
Embodiment 3
[0038] Example 3 Preparation of cell cryopreservation solution for human neural stem cells
[0039] It is composed of cryopreservation solution A and cryopreservation solution B, wherein, cryopreservation solution A is composed of the following components: 30% albumin, 4.0g / L glucose, 0.8mg / L vitamin B12, 0.365mg / L glutamine Amide, 6.0g / L sodium chloride, 0.030g / L magnesium chloride, 0.2g / L potassium chloride, and the balance is water.
[0040] Freezing solution B is composed of the following components: 20% dimethyl sulfoxide, 4.0g / L glucose, 0.8mg / L vitamin B12, 0.365mg / L glutamine, 6.0g / L sodium chloride, 0.030 g / L magnesium chloride, 0.2g / L potassium chloride, and the balance is water.
[0041] The preparation method of cell cryopreservation solution is as follows: prepare cryopreservation solution A and cryopreservation solution B respectively; add each component into ultrapure water, mix and dissolve, and shake and mix with an oscillator for 2 to 3 minutes during the diss...
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