Method for repairing saline-alkali soil by culturing indigenous microorganisms and application of method
A technology for microbial remediation and saline-alkali land, applied in the field of environmental microorganisms, can solve problems such as risks caused by exogenous microorganisms, and achieve the effects of improving remediation efficiency, simple process, and avoiding ecological pollution
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Embodiment 1
[0083] This embodiment provides a method for cultivating indigenous microorganisms to repair saline-alkali soil, comprising the following steps:
[0084] (1) Sampling 500g of the soil at detection points 1, 2, and 3 of the saline-alkaline land respectively, the sampling depth is 0-50cm, fully mix, weigh 500g after mixing and place it in a bucket with stirring, add 5L deionized water, and Stir at 80r / min for 30min, discard the bottom sludge, and obtain a saline-alkali soil suspension;
[0085] (2) Take 4.5L of the soil suspension obtained in step (1) and add it to a bucket with stirring, then add 450L of deionized water, add 45L of nutritional enhancer, stir for 10min every 2h, and continuously cultivate for 3d to obtain indigenous microorganisms Amplification solution 495.5L;
[0086] (3) Add 100L nutrient enhancer to the 495.5L indigenous microorganism amplification solution described in step (2), and spray evenly to 750m through pipeline spraying facilities 2 In the soil o...
Embodiment 2
[0090] This embodiment provides a method for cultivating indigenous microorganisms to repair saline-alkali soil, comprising the following steps:
[0091] (1) Sampling 500g of the soil at detection points 1, 2, and 3 of saline-alkali land respectively, the sampling depth is 0-50cm, fully mix, weigh 500g after mixing and place it in a bucket with stirring, add 8L deionized water, and Stir at 60r / min for 40min, discard the bottom sludge, and obtain a saline-alkali soil suspension;
[0092] (2) Take 5L of the soil suspension obtained in step (1) and add it to a bucket with stirring, then add 500L of deionized water, add 75L of nutritional enhancer, stir for 8min every 1.5h, and continuously cultivate for 5d to obtain indigenous microorganisms Amplification solution 580L;
[0093] (3) Add 145L nutrient enhancer to the 580L indigenous microorganism amplification solution described in step (2), and spray evenly to 750m through pipeline spraying facilities 2 In the soil of saline-al...
Embodiment 3
[0097] This embodiment provides a method for cultivating indigenous microorganisms to repair saline-alkali soil, comprising the following steps:
[0098] (1) Sampling 600g of the soil at detection points 1, 2, and 3 of the saline-alkaline land respectively, the sampling depth is 0-50cm, fully mix, weigh 600g after mixing and place it in a bucket with stirring, add 4L deionized water, and Stir at 100r / min for 30min, discard the bottom sludge, and obtain a saline-alkali soil suspension;
[0099] (2) Take 20L of the soil suspension obtained in step (1) and add it to a bucket with stirring, then add 1800L of deionized water, add 360L of nutritional enhancer, stir for 15min every 3h, and continuously cultivate for 2 days to obtain indigenous microorganisms Amplification solution 2180L;
[0100] (3) Add 650L nutrient enhancer to the 2180L indigenous microbial amplification solution described in step (2), and spray evenly to 750m through pipeline spraying facilities 2 In the soil o...
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