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Method by using tissue culture technique for redifferentiation of garlic into plant material

A technique of tissue culture and redifferentiation, applied in botany equipment and methods, plant regeneration, horticultural methods, etc., can solve the problems of poor practicability and low efficiency, and achieve the effect of strong practicability, advanced technology and easy operation

Inactive Publication Date: 2004-08-11
曹晓丽
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a kind of efficient method that garlic redifferentiates the plant body, to overcome the problems of low efficiency and poor practicability in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0011] Example 1: Store non-toxic garlic at 4°C for more than 2 weeks, peel off the epidermis, cut off excess parts, and then sterilize the garlic epidermis. After disinfection, cut off the stems of the scale discs including the leaf axils by 1-3 mm on the ultra-clean workbench, then divide them into 10 small pieces, and culture them on LS medium. After 7 to 10 days, the sliced ​​tissue begins to differentiate, and each piece grows 3 adventitious buds. After 3 weeks, each bud is cultured separately, and after 90 days, it can become 30 small balls that are the same as the bulbs.

example 2

[0012] Example 2: process steps and conditions are the same as example 1. Slice the stem part of the scale disk and divide it into 8 small pieces, each piece grows 2 adventitious buds to obtain 16 small balls.

example 3

[0013] Example 3: process steps and conditions are the same as example 1. Scale disc stem section is divided into 8 small pieces, and each piece grows 3 adventitious buds, then obtains 24 small balls.

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PUM

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Abstract

A process for obtaining the redifferentiated plant body of garlic by tissue culture technique in order to culture non-virus garlic features tha the tissue of the undifferentiated garlic cell taken from the squamula stem disk at least axil position is used to culture. Its advantages are high effect, short culture time and high yield.

Description

Technical field [0001] The present invention relates to a method for redifferentiated plant body using culture material using scale disc stem part including garlic leaf axil. Background technique [0002] Garlic, like potatoes, is a plant that grows by growing bulbs. Planting a scale yields 6 garlic slices. That is to say, if you use ordinary multiplication methods, you can only increase it by 6 times. Moreover, in recent years, garlic in my country has been commonly infected with viruses such as garlic mosaic virus. It makes it difficult for our country's garlic to reach export standards due to its small size and low yield. Therefore, many research institutes in our country have begun to study the detoxification of garlic, and the cultivation of growth points is one of the methods to remove viruses from garlic. That is to say, even if it is a virus-contaminated plant, its growth point is virus-free. Therefore, as long as the growth point is cultivated and the plant bod...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 曹晓丽
Owner 曹晓丽
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