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Fruit preserving agent produced from extract of plant of phytolaccaceae, its preparation and use

A plant-based and preservative technology, applied in the field of plant-based pollution-free fruit preservatives and pollution-free fruit preservatives, can solve the problem of no active ingredients, and achieve the effects of avoiding rot and deterioration and achieving good control effects.

Inactive Publication Date: 2005-02-23
CHONGQING ACAD OF CHINESE MATERIA MEDICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these patents did not involve the plant Pokeweed, and except for the patents disclosed by the Japanese in my country, which clearly used isovitexin a

Method used

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  • Fruit preserving agent produced from extract of plant of phytolaccaceae, its preparation and use

Examples

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Embodiment 1

[0033] Example 1 Selection of pokeweed species and application parts

[0034] In the present invention, the active ingredient that can play the role of sterilization and preservation is mainly the saponins contained in pokeweed plants, and the amount of saponins contained in different types of plants will directly affect the extraction rate. It will have an impact on the economic benefits of developing medicines. Plants of Phytolaccaceae (Phytolaccaceae) are herbs or shrubs, rarely trees. There are 17 genera and about 120 species in the world. There are only 2 genera and 4 species in my country, which are Phytolacca acinosa Roxb, P. .americana L.), Pokeweed (P.polyandra Batalin) and Pokeweed (P.japonica Mokino), among which Pokeweed is endemic to my country, and Pokeweed is the most common. The present invention determines that Pokeweed (P.americana L.) is an ideal plant for extracting saponins, because the root system of this plant is relatively developed, it grows rapidly, a...

Embodiment 2

[0035] Example 2 Extraction method and content detection of pokeweed saponins

[0036] The extraction method of pokeweed saponin compound is a lot, and the extraction method that the present invention relates to pokeweed saponin compound is:

[0037] Use ethanol as a solvent to extract the crushed product of fresh peduncle root, concentrate and dissolve the extract in water, filter to remove the insoluble matter, add petroleum ether to the aqueous solution for two-phase extraction, and transfer the lipophilic impurities to the lipophilic in the petroleum ether, while the saponins remained in the aqueous phase. After extracting the water phase after removing the ester-soluble impurities, and then further extracting with butanol with strong hydrophilicity, the saponin will be dissolved in butanol, while some impurities with strong hydrophilicity will remain in the water phase. The butanol solution is concentrated under reduced pressure to obtain milky white (slightly yellow) cr...

Embodiment 3

[0040] Example 3 Extraction method of pokeweed antifungal protein compound

[0041] Chop Phytolacca root, add 10 times the amount of 0.5 mol / l sodium chloride solution, homogenize with a high-speed tissue masher, and place at 4°C overnight. The homogenate was filtered through gauze to remove slag, the filtrate was refrigerated and centrifuged at 1000rpm for 20min, and the supernatant was placed in a 3.5kD dialysis bag and dialyzed against distilled water for 36h at low temperature. The precipitate was removed by centrifugation, the supernatant was adjusted to pH 5.5, and the sample was loaded on a CM-Sephadex C50 column equilibrated with 20 mmol / l citric acid buffer (pH 5.0) for chromatography. Discard the effluent, carry out two-step elution with 50mmol / l phosphate buffer (PB, pH7.0) and two solutions of 0.5mol / l sodium chloride in PB, obtain the pokeweed antifungal protein composition components.

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Abstract

A vegetative antistaling agent for the fruits (orange, apple, pear, etc) is prepared from the extracts of radix phytolaccae, such as phytolaccatoxin, anti-fungal protein etc, imidazolyl mould, 2,4-D sodium salt, emulsifier NP-10 and the rejected liquid containing honey. Its preparing process is also disclosed.

Description

technical field [0001] The invention relates to the technical field of botanical pollution-free fruit fresh-keeping agents, in particular to a pollution-free fruit fresh-keeping agent developed with the antibacterial active compound pokeweed saponin and antiviral protein of the plant pokeweed, a preparation method and its application. technical background [0002] Searching for antibacterial and bactericidal active substances from plants is one of the hot spots in the development of new pollution-free pesticides. Wilkings and Boa. rd (1989) reported that there are 1389 kinds of plants that may be used as fungicides or plant growth regulators, which contain many different types of compounds. Many noteworthy antimicrobial agents have been developed as natural secondary metabolites from plants. There are abundant resources of traditional Chinese medicine plants in our country. Screening the traditional Chinese medicine plants that can inhibit the main pathogenic bacteria duri...

Claims

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Application Information

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IPC IPC(8): A23B7/14A23L3/3472
Inventor 陈仕江丁伟金仕勇张勇强钟国跃马开森
Owner CHONGQING ACAD OF CHINESE MATERIA MEDICA
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