Method for quick breeding eel grass

A bitter grass and fast technology, which is applied in the application field of biotechnology in the restoration of water body vegetation and the production of aquatic weed seedlings in water cluster boxes, can solve the problem that it is difficult to meet the needs of water body vegetation restoration plant materials, the reproduction coefficient is low, and the scale cannot be scaled. production and other issues

Inactive Publication Date: 2005-03-02
WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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Problems solved by technology

In nature, the reproduction coefficient is low. If the conventional division method is used for reproduction, large-scale production cannot be formed, and it is difficult to meet the needs of water vegetation restoration and plant materials in water cluster boxes.

Method used

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Experimental program
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Embodiment Construction

[0033] A method for rapid propagation of bitter grass, the method is carried out according to the following steps:

[0034] (1) getting the underground stem tip of the bitter grass with a length of 2 to 3 centimeters as the bitter grass culture;

[0035] (2) Rinse the culture of bitter grass with running water for 20-30 minutes, then scrub the surface of the culture of bitter grass with 75% alcohol cotton balls, then soak it in 0.1% mercuric chloride for 5-8 minutes, and rinse with sterile water Rinse for 8-10 minutes;

[0036] (3) Introduce the culture of Erythria galeria treated by step (2) into the induction medium, and cultivate for 20 to 30 days, and the culture of Erythria galenia begins to germinate and grow; the induction medium is: add in every liter of MS basic medium 0.1 mg of KT (6-furanaminopurine), 0.1 mg of 2,4-D (2,4-dichlorophenoxyacetic acid), 30 grams of sucrose, 6 grams of agar; each liter of MS basic medium contains:

[0037] NH 4 NO 3 (ammonium nitrat...

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Abstract

The present invention discloses the quick breeding method of eel grass. The underground stem tip of eel grass as the cultured matter is cultured through disinfection, inducing culture, differentiation, proliferation and rooting culture to propagate eel grass quickly. Over 10,000 seedlings of eel grass may be cultured in one year with one underground stem tip of eel grass. The present invention makes it possible to provide great amount of eel grass seedlings for restoring water vegetation and decorating aquarium.

Description

Technical field: [0001] The invention relates to a tissue culture method of aquatic plants, more specifically to a rapid propagation method of bitter grass Hydrocharitaceae Vallisnera Linn., which belongs to the application of biotechnology in the restoration of water body vegetation and the production of aquatic plant seedlings in water cluster boxes. Background technique: [0002] Hydrocharitaceae Vallisnera Linn. belongs to the genus Hydrocharitaceae Vallisnera Linn. Its leaves are emerald green or slightly purple-red for ornamental purposes, and are relatively resistant to pollution. It is an important plant resource in the biological food chain and the main pioneer aquatic plant in the restoration of water body vegetation. . The leaves of bitter grass are linear or ribbon-shaped, lifelike in clear water, and it is also a commonly used species in water cluster tanks. In nature, the reproduction coefficient is low, such as using the conventional division method to reprod...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H3/04A01H4/00
Inventor 赵家荣徐惠珠刘艳玲徐立铭
Owner WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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