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Fast process for determining material milk bacteria total number

A technology for rapid determination of the total number of bacteria, applied in the field of detection, can solve problems such as inability to quantitatively express, and achieve the effects of fast detection speed, good cost performance, and low environmental requirements

Inactive Publication Date: 2006-08-30
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the application of this principle to detect the total number of bacteria in the dairy industry at home and abroad is still at the stage of visual colorimetry, and the judgment of the results is subjective and cannot be expressed quantitatively.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Steps:

[0028] (1) Add 1.8ml of raw milk solution to a cuvette, preheat at 37°C±1°C for 5 minutes, preheat and treat with light at the same time.

[0029] (2) During the measurement process, keep a constant temperature of 37°C±1°C.

[0030] (3) Add 0.2 ml of 0.005% resazurin reagent to the milk, blow in nitrogen gas and mix for 1 minute.

[0031] (4) Immediately after mixing, the color sensor is used to collect the color information of the mixed sample.

[0032] (5) After 10 minutes, collect the color information of the mixed sample.

[0033] (6) The computer processes and analyzes the color difference value before and after the color change, compares it with the standard, and reads the total number of bacteria in the milk sample to be tested.

Embodiment 2

[0035] Steps:

[0036] (1) Add 1.9ml of raw milk solution to a cuvette, preheat at 37°C±1°C for 3 minutes, and treat with light while preheating.

[0037] (2) During the measurement process, keep a constant temperature of 37°C±1°C.

[0038] (3) Add 0.1 ml of 0.01% resazurin reagent to the milk, blow in nitrogen gas and mix for 2 minutes.

[0039] (4) Immediately after mixing, the color sensor is used to collect the color information of the mixed sample.

[0040] (5) After 8 minutes, collect the color information of the mixed sample.

[0041] (6) The computer processes and analyzes the color difference value before and after the color change, compares it with the standard, and reads the total number of bacteria in the milk sample to be tested.

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PUM

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Abstract

This invention provides a quick determining material milk bacteria number method. (1) quantitative material milk solution is added in transparent vessel, then they are pre-heated 2-10minitues at 36 to 37 degree centigrade, and the temperature should be maintained at 36 to 37 degree centigrade during determine process. (2) Proportional oxidizing reducing reagent is added into the milk, then nitrogen gas is let in and mixed for 10 seconds to 4 minutes. (3) Color sensor is used to collect color information of the mixed sample after they are mixed. (4) The color information is collected again after 2 to 15minutes. Color differential value of the two collections is analyzed by computer and compared to standard value to get the total count of bacteria colonies of measuring milk. In this invention, the detecting speed is quickly, detecting limitation is low, the apparatus has good performance-price ratio, and its operation is simple.

Description

(1) Technical field [0001] The invention relates to a detection method, in particular to a rapid determination method applied to the detection of the total number of bacteria in raw milk. (2) Background technology [0002] Milk is rich in nutrients and is susceptible to microbial contamination. The number of bacteria in raw milk is closely related to product quality. Therefore, the total number of bacteria is an important indicator to measure the quality of raw milk. Online detection of it can help control the quality of raw milk and ensure product safety. [0003] At present, there are many detection methods for the total number of bacteria in the dairy industry, such as standard plate counting method, direct microscope counting method, reduction test method, flow cytometry method, etc. Each method has its own advantages and disadvantages. The most widely used method in China is the standard plate counting method. This method has the disadvantages of cumbersome operation an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/06
Inventor 霍贵成田波姜瞻梅王玉堂刘丽波
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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