Method for in vitro preparation of hepatocyte induced by umbilical cord blood hematopoietic stem cell

A technology of hematopoietic stem cells and hepatocyte growth factors, which is applied in the field of in vitro induction of hepatocytes, can solve the problems of stem cells not being suitable for growth and insufficient amount of hepatocyte transplantation cells, and achieve obvious effects and high proportions
CN1844373AInactive Publication Date: 2006-10-11EAST CHINA UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
EAST CHINA UNIV OF SCI & TECH
Publication Date
2006-10-11
Estimated Expiration
Not applicable · inactive patent

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Abstract

This invention discloses a method for producing liver cells by inducing haemopoietic stem cells in vitro, comprising: inoculating the haemopoietic stem cells or CD34+ cells into the culture media containing embryonic bovine serum, human stem cell factor, human interleukins 3(IL-3), interleukins 6(IL-6), human liver cell growth factor, human epidermal growth factor(EGF), human acdic fibroblast growth factor, human basic fibroblast growth factor(bFGF), human leukemia inhibitory factor(LIF) and oncostatin M(OSM), and inducing the producing of liver cells in vitro. By applying the method of this invention to induce liver cells from the haemopoietic stem cells, it can acquire a high proportion of liver cells in the culture with good effects.
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Description

technical field

[0001] The invention relates to a method for inducing the generation of hepatocytes in vitro, in particular to a method for inducing the generation of hepatocytes from umbilical cord blood hematopoietic stem cells in vitro. Background technique

[0002] As we all know, a large number of people die every year due to various liver diseases, and the number of patients with liver injury, cirrhosis and liver failure is even greater.

[0003] The liver is one of the important organs of the human body. Its functions on the human body are mainly reflected in three aspects: (1) Secretion of bile: liver cells continuously generate bile acids and secrete bile to help the digestion and absorption of fat in the small intestine. (2) Metabolic function: This mainly includes glucose metabolism, protein metabolism, fat metabolism, vitamin metabolism and hormone metabolism. (3) Detoxification function: the liver is the main detoxification organ in the human body, which can pr...

Claims

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