Liquid chromatography component

Abstract

The present invention aims to provide a liquid chromatography component including a column and a prefilter, which is hard to cause an increase of supplied liquid pressure even when the measurement of a sample is repeated. The present invention is a liquid chromatography component, which includes: a column with filler particles filled therein; and a prefilter, the filler particles having an average particle size in the range of 2 to 20 μm, the prefilter having a filtering particle size in the range of ⅙ to ⅓ of the average particle size of the filler particles.

Description

TECHNICAL FIELD[0001]The present invention relates to a liquid chromatography component including: a column with filler particles filled therein; and a prefilter, wherein the component is hard to cause an increase in the supplied liquid pressure even when the measurement of a sample is repeated. The present invention also relates to a liquid chromatography component including: a column with filler particles filled therein; and a prefilter, wherein the prefilter requires replacement at almost the same frequency as the column does. Further, the present invention relates to a liquid chromatography component including: a column with filler particles filled therein; and a prefilter, wherein the column and the prefilter are integrated with each other, require no complicated replacement operation, and show excellent separation performance.BACKGROUND ART[0002]In the fields of organic chemistry, biochemistry, medicine, and the like, liquid chromatography has been widely used for measurement ...

AI Technical Summary

Benefits of technology

"The present invention is about a liquid chromatography component that includes a column and a prefilter. The prefilter is designed to capture foreign substances and prevent them from clogging the column. The prefilter requires replacement at the same frequency as the column, which is hard to manage and can cause an increase in supplied liquid pressure if not done properly. The invention also integrates the column and prefilter, which simplifies the replacement operation and improves separation performance."

Problems solved by technology

These foreign substances are adsorbed on the surface of filler particles filled in the column or to a detector cell, and as a result, may adversely affect the measurement or analysis.

However, when the filtration efficiency of the filters is increased in order to more efficiently capture the foreign substances, the foreign substances may more easily clog the filters to cause the change in the supplied liquid pressure.

Particularly when a large number of samples are continuously measured as in analysis of hemoglobins or when a sample high in foreign substances like a hemolyzed blood sample is measured, the foreign substances clog the filter and often cause an increase in the supplied liquid pressure.

The change in the supplied liquid pressure may lead to a failure in accurate and quick measurement or analysis of the sample.

However, when the filtration area or the porosity of the filter is increased too much, the sample or the mobile phase in the filter may be diffused too much, which reduces the accuracy of the measurement or analysis.

However, these ideas have been established by taking only the filter into consideration, and no studies have been made covering the column where the clogging attributed to the foreign substances poses a problem as in the filter.

Thus, the filters require the replacement more often than the column does, which is a significant burden in terms of operation and cost.Patent Document 1: Japanese Kokai Publication No.

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