Fibrosis causing agent
a fibrosis-causing agent and agent technology, applied in the field of fibrosis-causing agents, can solve the problem that does not necessarily produce the effect of reducing lung capacity as desired, and achieve the effect of reducing lung capacity
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example 1
[0154]Experiments for fibrosis were carried out in which the fibrosis inducer is sodium alginate and the fibrosis promoter is platelet-rich plasma (PRP).
[0155](Preparation of Aqueous Solution of Sodium Alginate)
[0156]An aqueous solution (0.5% w / v) of sodium alginate was prepared by dissolving 0.15 g of sodium alginate (made by Wako Pure Chemical Industries, Ltd.) in 30 mL of reverse osmosis water (RO water), followed by filtration and sterilization through a sterilizing filter (Millipore 0.22 μm). The resulting solution was a viscous fluid.
[0157]Preparation of Platelet-Rich Plasma (PRP)
[0158]In the first step, 10 mL each of blood was collected into two syringes (10 mL) containing 1.0 mL of anticoagulant ACD-A solution (citric acid glucose solution, made by Terumo Corp.) from the auricular artery of unanesthetized Japanese white rabbits (clean, male, 3.0 to 3.49 kg). The collected blood was transferred to a 15-mL centrifuge tube, which was slowly tumbled for mixing.
[0159]The blood wa...
example 2
[0173]Experiments for fibrosis were carried out in which the fibrosis inducer is sodium alginate and the fibrosis promoter is platelet-rich plasma (PRP). These components were used in combination with calcium chloride as the PRP gelling agent.
[0174]Preparation of aqueous solution of sodium alginate and preparation of platelet-rich plasma (PRP)
[0175]The same procedure as in Example 1 was repeated to prepare the aqueous solution of sodium alginate and the platelet-rich plasma (PRP).
[0176]Preparation of Mixed Solution
[0177]A mixed solution was prepared from the aqueous solution of sodium alginate and the platelet-rich plasma (PRP) which were mixed together in a ratio of 1:1 (by volume). The resulting mixed solution was a viscous liquid.
[0178]Preparation of Aqueous Solution of Calcium Chloride
[0179]An aqueous solution (40 mM) of calcium chloride was prepared by dissolving 0.222 g of calcium chloride (made by Wako Pure Chemical Industries, Ltd.) in reverse osmosis water (RO water) such t...
example 3
[0215]Experiments for fibrosis were carried out in which the fibrosis inducer is iron alginate and the fibrosis promoter is platelet-rich plasma (PRP). These components were used in combination with calcium chloride as the PRP gelling agent.
[0216]Preparation of Aqueous Dispersion of Iron Alginate
[0217]An aqueous solution (1% w / v) of iron chloride was prepared by dissolving 5.0 g of iron chloride (made by Wako Pure Chemical Industries, Ltd.) in 500 mL of reverse osmosis water (RO water).
[0218]Also, an aqueous solution (1% w / v) of sodium alginate was prepared by dissolving 1.5 g of sodium alginate (made by Wako Pure Chemical Industries, Ltd.) in 150 mL of reverse osmosis water (RO water).
[0219]The aqueous solution of sodium alginate (in atomized form) was added to the aqueous solution of iron chloride being swirled, so that there was obtained iron alginate in particulate form. The aqueous dispersion of iron alginate was sifted through a sieve with an opening of 100 μm and then thoroug...
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