Method for separating hydroquinone from alpha-arbutin fermentation solution

Abstract

The invention provides a method for separating hydroquinone from an alpha-arbutin fermentation solution. The method comprises the following steps: (1) tributyl phosphate is used as an extracting agent for extracting the alpha-arbutin fermentation solution, and further an extract phase and a raffinate phase are separated, wherein the volume ratio of the extracting agent to the alpha-arbutin fermentation solution is 1 to (0.5-2) and the extract phase contains the hydroquinone; (2) stripping the extract phase obtained in the step (1) by utilizing a strip liquor, so as to separate the hydroquinone from the extract phase conveniently, wherein the strip liquor is a basic solution or an acid solution and the volume ratio of the extract phase and the stripping liquor is 1 to (0.01-0.5). With adoption of the method, the hydroquinone in the alpha-arbutin fermentation solution can be extracted efficiently at low cost, the impurity removing rate of the hydroquinone can be improved, the highly purified alpha-arbutin can be obtained, the emission of wastewater is reduced, and the method has an important significance on improving the economical efficiency of alpha-arbutin prepared through a biologic method.

Description

technical field

[0001] The invention relates to the field of biochemical industry, more specifically, the invention relates to a method for separating hydroquinone from α-arbutin fermentation liquid. Background technique

[0002] α-Arbutin belongs to hydroquinone glycosides, its chemical name is 4-hydroxyphenyl-D-glucopyranoside, and its whitening activity is ten times that of β-arbutin. Researchers found that arbutin, as an inhibitor of tyrosinase, can block the synthesis of dopa and dopaquinone, thereby effectively inhibiting the production of melanin, has whitening effect, and is not irritating to the skin, with little side effects. It has the effect of significantly inhibiting the activity of tyrosinase. It is an emerging non-irritating, non-allergic and compatible natural whitening active substance. The recommended dosage of arbutin in cosmetics is 1%~5%, which has a high economical effect. value.

[0003] Hydroquinone (hydroquinone) chemical name hydroquinone, its mo...

Examples

Embodiment 1

[0031] In this example, tributyl phosphate is selected as the extractant, and α-arbutin fermentation liquid is used as raw material, wherein α-arbutin 20g / L, sodium chloride 64 g / L, pH5.5, hydroquinone 5.0g / L, the specific extraction recovery method is carried out according to the following steps.

[0032] (1) The α-arbutin fermentation broth and tributyl phosphate were subjected to three-stage countercurrent liquid-liquid extraction at 25°C at a volume ratio of 1:1, and the extraction phase and raffinate phase were collected after the extraction was completed.

[0033] (2) Add the extract phase and sodium hydroxide solution obtained in step (1) into the extraction tower at a volume ratio of 1:0.05. The operating temperature of the extraction tower is 35°C and the operating pressure is -0.1 Mpa. The top effluent is cooled and stratified. The upper organic phase can be reused as the extraction agent, and the lower aqueous phase can be recovered.

[0034] (3) The hydroquinone s...

Embodiment 2

[0040]In this example, tributyl phosphate was selected as the extractant, and α-arbutin fermentation liquid was used as raw material, wherein α-arbutin 30g / L, ammonium sulfate 20g / L, pH 5.0, hydroquinone 5.0g / L, the specific extraction recovery method is carried out according to the following steps.

[0041] (1) The α-arbutin fermentation broth and tributyl phosphate were subjected to liquid-liquid extraction at a volume ratio of 1:2 at 30°C. After the extraction was completed, it was left to stand until the two phases were separated. The two phases were separated, and the upper extract phase and the lower raffinate phase were collected.

[0042] (2) Add the extract phase obtained in step (1) and sodium hydroxide solution into the extraction tower at a volume ratio of 1:0.1. The operating temperature of the extraction tower is 30°C and the operating pressure is -0.1 Mpa. The top effluent is cooled and stratified. The upper organic phase can be reused as the extraction agent,...

Embodiment 3

[0048] In this example, propyl formate is selected as the extractant, and α-arbutin fermentation liquid is used as raw material, wherein α-arbutin is 24 g / L, magnesium sulfate is 40 g / L, pH5.5, and hydroquinone is 5.2 g / L, the specific extraction recovery method is carried out according to the following steps.

[0049] (1) The α-arbutin fermentation broth and tributyl phosphate were subjected to liquid-liquid extraction at a volume ratio of 1:2 at 35°C. After the extraction was completed, it was left to stand until the two phases were separated. The two phases were separated, and the upper extract phase and the lower raffinate phase were collected.

[0050] (2) Add the extract phase obtained in step (1) and sodium hydroxide solution into the extraction tower at a volume ratio of 1:0.04. The operating temperature of the extraction tower is 30°C and the operating pressure is -0.5 Mpa. The top effluent is cooled and stratified. The upper organic phase can be reused as the extrac...

technical field

[0001] The invention relates to the field of biochemical industry, more specifically, the invention relates to a method for separating hydroquinone from α-arbutin fermentation liquid. Background technique

[0002] α-Arbutin belongs to hydroquinone glycosides, its chemical name is 4-hydroxyphenyl-D-glucopyranoside, and its whitening activity is ten times that of β-arbutin. Researchers found that arbutin, as an inhibitor of tyrosinase, can block the synthesis of dopa and dopaquinone, thereby effectively inhibiting the production of melanin, has whitening effect, and is not irritating to the skin, with little side effects. It has the effect of significantly inhibiting the activity of tyrosinase. It is an emerging non-irritating, non-allergic and compatible natural whitening active substance. The recommended dosage of arbutin in cosmetics is 1%~5%, which has a high economical effect. value.

[0003] Hydroquinone (hydroquinone) chemical name hydroquinone, its mo...

Examples

Embodiment 1

[0031] In this example, tributyl phosphate is selected as the extractant, and α-arbutin fermentation liquid is used as raw material, wherein α-arbutin 20g / L, sodium chloride 64 g / L, pH5.5, hydroquinone 5.0g / L, the specific extraction recovery method is carried out according to the following steps.

[0032] (1) The α-arbutin fermentation broth and tributyl phosphate were subjected to three-stage countercurrent liquid-liquid extraction at 25°C at a volume ratio of 1:1, and the extraction phase and raffinate phase were collected after the extraction was completed.

[0033] (2) Add the extract phase and sodium hydroxide solution obtained in step (1) into the extraction tower at a volume ratio of 1:0.05. The operating temperature of the extraction tower is 35°C and the operating pressure is -0.1 Mpa. The top effluent is cooled and stratified. The upper organic phase can be reused as the extraction agent, and the lower aqueous phase can be recovered.

[0034] (3) The hydroquinone s...

Embodiment 2

[0040]In this example, tributyl phosphate was selected as the extractant, and α-arbutin fermentation liquid was used as raw material, wherein α-arbutin 30g / L, ammonium sulfate 20g / L, pH 5.0, hydroquinone 5.0g / L, the specific extraction recovery method is carried out according to the following steps.

[0041] (1) The α-arbutin fermentation broth and tributyl phosphate were subjected to liquid-liquid extraction at a volume ratio of 1:2 at 30°C. After the extraction was completed, it was left to stand until the two phases were separated. The two phases were separated, and the upper extract phase and the lower raffinate phase were collected.

[0042] (2) Add the extract phase obtained in step (1) and sodium hydroxide solution into the extraction tower at a volume ratio of 1:0.1. The operating temperature of the extraction tower is 30°C and the operating pressure is -0.1 Mpa. The top effluent is cooled and stratified. The upper organic phase can be reused as the extraction agent,...

Embodiment 3

[0048] In this example, propyl formate is selected as the extractant, and α-arbutin fermentation liquid is used as raw material, wherein α-arbutin is 24 g / L, magnesium sulfate is 40 g / L, pH5.5, and hydroquinone is 5.2 g / L, the specific extraction recovery method is carried out according to the following steps.

[0049] (1) The α-arbutin fermentation broth and tributyl phosphate were subjected to liquid-liquid extraction at a volume ratio of 1:2 at 35°C. After the extraction was completed, it was left to stand until the two phases were separated. The two phases were separated, and the upper extract phase and the lower raffinate phase were collected.

[0050] (2) Add the extract phase obtained in step (1) and sodium hydroxide solution into the extraction tower at a volume ratio of 1:0.04. The operating temperature of the extraction tower is 30°C and the operating pressure is -0.5 Mpa. The top effluent is cooled and stratified. The upper organic phase can be reused as the extrac...