Application of gingko extract nanometer liposome in bromidrosis treatment

Abstract

The invention relates to a use of gingko extract nanometer liposome in preparation of external application preparations for bromidrosis treatment. According to the present invention, the gingko extract can be obtained from extracts of leaf, fruit, exopleura, rhizome and other parts of gingko through a chemical separation method; the gingko extract nanometer liposome is prepared by using a film ultrasound-high pressure homogenization method; the drug, the cosmetic or the disinfectant external application preparation containing the gingko extract nanometer liposome and provided for bromidrosis treatment contains 0.001-80% of the gingko extract nanometer liposome and a pharmaceutically and commonly used external application preparation substrate or an acceptable carrier; the preparation is the any one external application formulation in the pharmacy; results of pharmacology studies show that the gingko extract nanometer liposome can inhibit apocrine gland cell proliferation and provides significant damage effects for apocrine gland cells, and the effect of the gingko extract nanometer liposome is significantly superior to the effect of the gingko extract external application preparation control group; and results of clinical trials show that the gingko extract nanometer liposome provides significant treatment effects for bromidrosis, wherein the effective rate is 100%, and characteristics of safe use and no side effect are provided.

Description

technical field

[0001] The invention belongs to the technical field of biomedicine and therapeutic cosmetics, and in particular relates to the application of ginkgo extract nano-liposomes in the treatment of bronchus. Background technique

[0002] Bromidrosis can be divided into generalized bronchial sweating and local bronchial sweating. Underarm odor is a common hereditary skin metabolic disease, commonly known as body odor, mainly manifested as armpit odor. The main cause of sweat odor is the excessive secretion of organic substances in the sweat excreted by the apocrine glands, such as protein, lipid and other components, which are decomposed by skin surface bacteria (Staphylococcus epidermidis, Staphylococcus aureus, etc.) to produce unsaturated fatty acids and microbes. Molecular ammonia nitrogen compounds and other metabolites produce unpleasant odors. Therefore, destroying the apocrine glands is considered to be the key to the cure of underarm odor. Bromohidrosis ...

Examples

Embodiment 1

[0020] Peel the ginkgo nuts and crush them in a tissue grinder. Accurately weigh a certain amount of crushed ginkgo fruit, add 80% ethanol with a solid-to-liquid ratio of 1:10 (W / V), and stir and extract twice at a water bath temperature of 60°C, each extraction time is 4 hours. After the leaching, centrifuge (5000 rpm, 15 minutes), and take the supernatant. Concentrate the supernatant with a vacuum rotary evaporator under the conditions of temperature 55°C, pressure 0.008-0.009MPa, concentrate under reduced pressure, and vacuum-dry at 60°C to obtain Ginkgo biloba extract. The properties are: light yellow extract, soluble in Alcohol, insoluble in water.

[0021] Phospholipid absolute ethanol solution, filter; add the above-mentioned ginkgo extract extract, vitamin E, shake evenly, put in a round bottom flask, 30 ℃ water bath constant temperature rotary evaporation, reduce pressure to form a film, so that the film material is uniformly formed on the bottle wall Add PBS buffer...

Embodiment 2

[0024] Peel the ginkgo nuts and crush them in a tissue grinder. Accurately weigh a certain amount of crushed ginkgo fruit, add distilled water with a solid-to-liquid ratio of 1:10 (W / V), and stir and extract twice at a water bath temperature of 60°C, each extraction time is 4 hours. After the leaching, centrifuge (5000 rpm, 15 minutes), and take the supernatant. Concentrate the supernatant, and dry it under vacuum at 60°C to obtain the ginkgo extract. The properties are: light yellow extract, soluble in alcohol, insoluble in water.

[0025] Ginkgo extract nanoliposome suspension was prepared according to the preparation process of Ginkgo extract nanoliposome suspension in Example 1.

[0026] Preparation of the gel: Sprinkle 10 g of Carbopol on the surface of 500 ml of distilled water, and swell overnight. Weigh 60 g of glycerin and 120 ml of propylene glycol, respectively add them to the swollen Carbopol, and grind them evenly to obtain a transparent gel matrix. Take 40ml o...

Embodiment 3

[0028] Peel the ginkgo nuts and crush them in a tissue grinder. Accurately weigh a certain amount of crushed ginkgo fruit, add 80% petroleum ether with a solid-liquid ratio of 1:10 (W / V), and stir and extract twice at a water bath temperature of 60°C, each extraction time is 4 hours. After the leaching, centrifuge (5000 rpm, 15 minutes), and take the supernatant. Concentrate the supernatant with a vacuum rotary evaporator under the conditions of temperature 55°C, pressure 0.008-0.009MPa, concentrate under reduced pressure, and vacuum-dry at 60°C to obtain Ginkgo biloba extract. The properties are: light yellow extract, soluble in Alcohol, insoluble in water.

[0029] Ginkgo extract nanoliposome suspension was prepared according to the preparation process of Ginkgo extract nanoliposome suspension in Example 1.

[0030] Preparation of ointment: Take 5ml of the above-mentioned ginkgo extract nanoliposome suspension, dissolve it in 100ml of 95% ethanol solution, add 50ml of trie...

technical field

[0001] The invention belongs to the technical field of biomedicine and therapeutic cosmetics, and in particular relates to the application of ginkgo extract nano-liposomes in the treatment of bronchus. Background technique

[0002] Bromidrosis can be divided into generalized bronchial sweating and local bronchial sweating. Underarm odor is a common hereditary skin metabolic disease, commonly known as body odor, mainly manifested as armpit odor. The main cause of sweat odor is the excessive secretion of organic substances in the sweat excreted by the apocrine glands, such as protein, lipid and other components, which are decomposed by skin surface bacteria (Staphylococcus epidermidis, Staphylococcus aureus, etc.) to produce unsaturated fatty acids and microbes. Molecular ammonia nitrogen compounds and other metabolites produce unpleasant odors. Therefore, destroying the apocrine glands is considered to be the key to the cure of underarm odor. Bromohidrosis ...

Examples

Embodiment 1

[0020] Peel the ginkgo nuts and crush them in a tissue grinder. Accurately weigh a certain amount of crushed ginkgo fruit, add 80% ethanol with a solid-to-liquid ratio of 1:10 (W / V), and stir and extract twice at a water bath temperature of 60°C, each extraction time is 4 hours. After the leaching, centrifuge (5000 rpm, 15 minutes), and take the supernatant. Concentrate the supernatant with a vacuum rotary evaporator under the conditions of temperature 55°C, pressure 0.008-0.009MPa, concentrate under reduced pressure, and vacuum-dry at 60°C to obtain Ginkgo biloba extract. The properties are: light yellow extract, soluble in Alcohol, insoluble in water.

[0021] Phospholipid absolute ethanol solution, filter; add the above-mentioned ginkgo extract extract, vitamin E, shake evenly, put in a round bottom flask, 30 ℃ water bath constant temperature rotary evaporation, reduce pressure to form a film, so that the film material is uniformly formed on the bottle wall Add PBS buffer...

Embodiment 2

[0024] Peel the ginkgo nuts and crush them in a tissue grinder. Accurately weigh a certain amount of crushed ginkgo fruit, add distilled water with a solid-to-liquid ratio of 1:10 (W / V), and stir and extract twice at a water bath temperature of 60°C, each extraction time is 4 hours. After the leaching, centrifuge (5000 rpm, 15 minutes), and take the supernatant. Concentrate the supernatant, and dry it under vacuum at 60°C to obtain the ginkgo extract. The properties are: light yellow extract, soluble in alcohol, insoluble in water.

[0025] Ginkgo extract nanoliposome suspension was prepared according to the preparation process of Ginkgo extract nanoliposome suspension in Example 1.

[0026] Preparation of the gel: Sprinkle 10 g of Carbopol on the surface of 500 ml of distilled water, and swell overnight. Weigh 60 g of glycerin and 120 ml of propylene glycol, respectively add them to the swollen Carbopol, and grind them evenly to obtain a transparent gel matrix. Take 40ml o...

Embodiment 3

[0028] Peel the ginkgo nuts and crush them in a tissue grinder. Accurately weigh a certain amount of crushed ginkgo fruit, add 80% petroleum ether with a solid-liquid ratio of 1:10 (W / V), and stir and extract twice at a water bath temperature of 60°C, each extraction time is 4 hours. After the leaching, centrifuge (5000 rpm, 15 minutes), and take the supernatant. Concentrate the supernatant with a vacuum rotary evaporator under the conditions of temperature 55°C, pressure 0.008-0.009MPa, concentrate under reduced pressure, and vacuum-dry at 60°C to obtain Ginkgo biloba extract. The properties are: light yellow extract, soluble in Alcohol, insoluble in water.

[0029] Ginkgo extract nanoliposome suspension was prepared according to the preparation process of Ginkgo extract nanoliposome suspension in Example 1.

[0030] Preparation of ointment: Take 5ml of the above-mentioned ginkgo extract nanoliposome suspension, dissolve it in 100ml of 95% ethanol solution, add 50ml of trie...

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