Application of peanut coat active component and composition comprising peanut coat active component

Abstract

The invention discloses an application of a peanut coat active component and a composition comprising the peanut coat active component. The peanut coat active component can be used for preparing foods and medicines for preventing and treating neurodegenerative diseases. The invention also provides a composition comprising the peanut coat active component and Omega-3 series unsaturated fatty acid. The invention provides a new application of the peanut coat active component, thus offering a new thinking for preparing foods and medicines for preventing and treating neurodegenerative diseases like Alzheimer's disease. In addition, the composition also contains omega-3 series unsaturated fatty acid besides the peanut coat active component, thus better improving memory function.

Description

technical field

[0001] The invention belongs to the technical field of food and medicine application, and in particular relates to an application of an active component of peanut coating and a composition containing the active component of peanut coating. Background technique

[0002] With the development of the global social population, the growth of the elderly population is a worldwide trend, and the prevalence and incidence of senile dementia are gradually increasing. Senile dementia is not only a medical problem, but will become a prominent social problem . At present, the number of Alzheimer's patients worldwide may have reached 17 million. With the aging of the population, the incidence of Alzheimer's disease has risen to the fourth common cause of death, second only to cardiovascular and cerebrovascular diseases, cancer and stroke. Senile dementia has gradually become a chronic disease that seriously threatens the health of the elderly in our country.

[0003] Seni...

Examples

Embodiment 1

[0048] The preparation of embodiment 1 peanut shell active component

[0049] (1) Extract 1,000 grams of dried peanut shells with 16 liters of alcohol-water mixed solvent containing 60% ethanol at 40°C for 1 hour, then filter to obtain the first filtrate;

[0050] (2) Add 2 liters of alcohol-water mixed solvent containing 60% ethanol to the residue, extract at 40°C for 30 minutes, and filter to obtain the second filtrate;

[0051] (3) The two filtrates were combined and then concentrated under reduced pressure at 40°C to 1 / 50 of the volume, and then absorbed with five liters of Diaion (Mitsubishi Chemical) HP-20 resin, and then eluted with 15 liters of water to remove sugar and other substances. 10 liters of aqueous solution containing 40% ethanol was eluted, and the eluate was collected;

[0052] (4) Concentrate the eluent under reduced pressure at 40°C to 1 / 50 volume, and then freeze-dry to a water content of 5% to obtain 115.8 g of the active ingredient of peanut coating. ...

Embodiment 2

[0058] The in vitro biological activity of embodiment 2 peanut shell active components

[0059] In animal models of neurodegenerative diseases, studies have found that nerve growth factor can promote nerve growth and neuroprotection, can prevent or reduce neuron degeneration, and can prevent the progression of Alzheimer's disease to a certain extent. Because PC12 cells have the general characteristics of nerve cells, under the action of nerve growth factor, PC12 cells will stop dividing, grow protrusions, and transform into neuron-like cells. Therefore, it is reliable to use PC12 cells as a biological activity identification system to screen active samples for the preparation of health food or medicine for the prevention and treatment of senile dementia.

[0060] (1) Culture of PC12 cells

[0061] Connect 20×10 4 PC12 cells were inoculated in 10ml DMEM medium (containing 10% horse serum, 5% fetal calf serum) culture dish, 37°C, 5% CO 2 Cultivate in an incubator, replace the...

Embodiment 3

[0070] The effect of the mixed use of embodiment 3 peanut shell active components and DHA on the memory function of mice

[0071] Experimental main materials: ICR mice (8-week-old male mice) (purchased from the Animal Center of Zhejiang Academy of Medical Sciences).

[0072] Experimental operation: Peanut coating active ingredient (10mg / kg) and docosahexaenoic acid (DHA) (5mg / kg) were orally administered separately or mixed (10mg / kg peanut coating active ingredient+5mg / kg DHA ), each treatment and control group were 10 mice, and the active ingredient of peanut shell and / or docosahexaenoic acid (DHA) were prepared in an aqueous solution containing 40% glycerol and 6% glycerol fatty acid ester. The solvent control group was the solvent mentioned above. The administration was once every 24 hours, the maximum volume of administration was 100 μl, and the administration frequency was 7 times. .

[0073] Detection of learning memory and spatial memory function - water maze test. ...

technical field

[0001] The invention belongs to the technical field of food and medicine application, and in particular relates to an application of an active component of peanut coating and a composition containing the active component of peanut coating. Background technique

[0002] With the development of the global social population, the growth of the elderly population is a worldwide trend, and the prevalence and incidence of senile dementia are gradually increasing. Senile dementia is not only a medical problem, but will become a prominent social problem . At present, the number of Alzheimer's patients worldwide may have reached 17 million. With the aging of the population, the incidence of Alzheimer's disease has risen to the fourth common cause of death, second only to cardiovascular and cerebrovascular diseases, cancer and stroke. Senile dementia has gradually become a chronic disease that seriously threatens the health of the elderly in our country.

[0003] Seni...

Examples

Embodiment 1

[0048] The preparation of embodiment 1 peanut shell active component

[0049] (1) Extract 1,000 grams of dried peanut shells with 16 liters of alcohol-water mixed solvent containing 60% ethanol at 40°C for 1 hour, then filter to obtain the first filtrate;

[0050] (2) Add 2 liters of alcohol-water mixed solvent containing 60% ethanol to the residue, extract at 40°C for 30 minutes, and filter to obtain the second filtrate;

[0051] (3) The two filtrates were combined and then concentrated under reduced pressure at 40°C to 1 / 50 of the volume, and then absorbed with five liters of Diaion (Mitsubishi Chemical) HP-20 resin, and then eluted with 15 liters of water to remove sugar and other substances. 10 liters of aqueous solution containing 40% ethanol was eluted, and the eluate was collected;

[0052] (4) Concentrate the eluent under reduced pressure at 40°C to 1 / 50 volume, and then freeze-dry to a water content of 5% to obtain 115.8 g of the active ingredient of peanut coating. ...

Embodiment 2

[0058] The in vitro biological activity of embodiment 2 peanut shell active components

[0059] In animal models of neurodegenerative diseases, studies have found that nerve growth factor can promote nerve growth and neuroprotection, can prevent or reduce neuron degeneration, and can prevent the progression of Alzheimer's disease to a certain extent. Because PC12 cells have the general characteristics of nerve cells, under the action of nerve growth factor, PC12 cells will stop dividing, grow protrusions, and transform into neuron-like cells. Therefore, it is reliable to use PC12 cells as a biological activity identification system to screen active samples for the preparation of health food or medicine for the prevention and treatment of senile dementia.

[0060] (1) Culture of PC12 cells

[0061] Connect 20×10 4 PC12 cells were inoculated in 10ml DMEM medium (containing 10% horse serum, 5% fetal calf serum) culture dish, 37°C, 5% CO 2 Cultivate in an incubator, replace the...

Embodiment 3

[0070] The effect of the mixed use of embodiment 3 peanut shell active components and DHA on the memory function of mice

[0071] Experimental main materials: ICR mice (8-week-old male mice) (purchased from the Animal Center of Zhejiang Academy of Medical Sciences).

[0072] Experimental operation: Peanut coating active ingredient (10mg / kg) and docosahexaenoic acid (DHA) (5mg / kg) were orally administered separately or mixed (10mg / kg peanut coating active ingredient+5mg / kg DHA ), each treatment and control group were 10 mice, and the active ingredient of peanut shell and / or docosahexaenoic acid (DHA) were prepared in an aqueous solution containing 40% glycerol and 6% glycerol fatty acid ester. The solvent control group was the solvent mentioned above. The administration was once every 24 hours, the maximum volume of administration was 100 μl, and the administration frequency was 7 times. .

[0073] Detection of learning memory and spatial memory function - water maze test. ...

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