Preparation method of double-quenched procalcitonin electrochemiluminescence sensor

Abstract

The invention relates to a sensor preparation method for procalcitonin detection based on resonance energy transfer-based antibody directional immobilization double-quenching electrochemiluminescence strategy, which belongs to the technical field of electrochemiluminescence detection. Molybdenum trioxide has a good quenching effect on ruthenium terpyridyl, and the successful combination of gold nanoparticles and molybdenum trioxide further enhances the quenching effect of molybdenum trioxide on the electrochemiluminescence signal of ruthenium terpyridine. The HWRGWVC polypeptide chain is used as a specific capture body to realize the directional site capture of antibodies. The detection of procalcitonin is realized according to the difference in intensity of electrochemiluminescent signal in response to different concentrations of procalcitonin. The recovery rate of different concentrations of procalcitonin in human serum was detected by the standard recovery method to demonstrate the accuracy and precision of the method. Accurate and reliable.

Description

technical field

[0001] The invention relates to the preparation and application of an electrochemiluminescent immunosensor based on gold-composite molybdenum trioxide hollow nanospheres quenching terpyridine ruthenium-loaded hollow multi-sheet indium sulfide, specifically the hollow multi-sheet terpyridine ruthenium-loaded A layer of indium sulfide is used as the luminescent material, and the gold-functionalized gold-complexed molybdenum trioxide hollow nanosphere is used as the quencher, and the polypeptide chain is used to realize the directional fixation of the antibody, and a quenching electrode for detecting procalcitonin is prepared. A chemiluminescence sensor belongs to the technical field of electrochemiluminescence detection. Background technique

[0002] SIRS refers to systemic inflammatory response, that is, the body's response to various cytokines / inflammatory mediators. Endotoxins are triggers of the systemic inflammatory response SIR, for which there is curren...

Examples

Embodiment 1

[0037] Example 1 Preparation of hollow multi-layer indium sulfide supported by terpyridine ruthenium

[0038] (1) Preparation of hollow multi-sheet indium sulfide

[0039] First prepare the precursor: 4.5 mg of indium nitrate hydrate and 5.5 mg of 1,4-terephthalic acid were dissolved in 2 mL of N,N-dimethylformamide, stirred for 10 min, and then the mixed solution was heated at 120°C After heating for 30 min at room temperature and cooling to room temperature, the resulting white precipitate was washed three times with ethanol and dried. Next, the precursor was added to 40 mL ethanol solution containing 200 mg of thiourea and stirred for 2 min. The mixture was transferred to a 80 mL polytetrafluoroethylene Reaction in a stainless steel autoclave lined with vinyl fluoride at 180 °C for 3 h, after cooling to room temperature, the precipitate was washed three times with ethanol and ultrapure water and dried to obtain a yellow powder that is hollow multi-layer indium sulfide;

[...

Embodiment 2

[0042] Example 2 Preparation of Procalcitonin Secondary Antibody Incubation Solution Labeled with Gold-complexed Molybdenum Trioxide Hollow Nanospheres

[0043] (1) Preparation of molybdenum trioxide hollow nanospheres

[0044] First, 0.12 g C 10 h 14 MoO 6 Sonicate in 20 mL of n-butanol for 10 min to disperse the solid into the solvent evenly, then add 5 mL of 1 M nitric acid to the mixed solution under magnetic stirring and continue stirring for 1 h to transfer the obtained mixed solution to into a 50 mL polytetrafluoroethylene-lined stainless steel autoclave, after that, heat at 220 °C for 12 h, and cool to room temperature, wash the black precipitate twice with deionized water and absolute ethanol successively, at 60 °C The precursor MoO was obtained after drying for 5 h 2 , and then heated at 300 °C in air atmosphere for 2 h, the obtained white MoO 3 powder;

[0045] (2) Preparation of gold-composite molybdenum trioxide hollow nanospheres

[0046] 1 mL of 1% HAuCl ...

Embodiment 3

[0049] Example 3 Preparation of Procalcitonin Secondary Antibody Incubation Solution Labeled with Gold-complexed Molybdenum Trioxide Hollow Nanospheres

[0050] (1) Preparation of molybdenum trioxide hollow nanospheres

[0051] First, 0.13 g C 10 h 14 MoO 6 Sonicate in 30 mL of n-butanol for 10 min to disperse the solid into the solvent evenly, then add 10 mL of 1 M nitric acid to the mixed solution under magnetic stirring and continue stirring for 1 h to transfer the obtained mixed solution to into a 50 mL polytetrafluoroethylene-lined stainless steel autoclave, after that, heated at 220 °C for 12 h, and cooled to room temperature, and washed the black precipitate 6 times with deionized water and absolute ethanol in sequence, and at 60 °C The precursor MoO was obtained after drying for 5 h 2 , and then heated at 550 °C in air atmosphere for 5 h, the obtained white MoO 3 powder;

[0052] (2) Preparation of gold-composite molybdenum trioxide hollow nanospheres

[0053] 2...

technical field

[0001] The invention relates to the preparation and application of an electrochemiluminescent immunosensor based on gold-composite molybdenum trioxide hollow nanospheres quenching terpyridine ruthenium-loaded hollow multi-sheet indium sulfide, specifically the hollow multi-sheet terpyridine ruthenium-loaded A layer of indium sulfide is used as the luminescent material, and the gold-functionalized gold-complexed molybdenum trioxide hollow nanosphere is used as the quencher, and the polypeptide chain is used to realize the directional fixation of the antibody, and a quenching electrode for detecting procalcitonin is prepared. A chemiluminescence sensor belongs to the technical field of electrochemiluminescence detection. Background technique

[0002] SIRS refers to systemic inflammatory response, that is, the body's response to various cytokines / inflammatory mediators. Endotoxins are triggers of the systemic inflammatory response SIR, for which there is curren...

Examples

Embodiment 1

[0037] Example 1 Preparation of hollow multi-layer indium sulfide supported by terpyridine ruthenium

[0038] (1) Preparation of hollow multi-sheet indium sulfide

[0039] First prepare the precursor: 4.5 mg of indium nitrate hydrate and 5.5 mg of 1,4-terephthalic acid were dissolved in 2 mL of N,N-dimethylformamide, stirred for 10 min, and then the mixed solution was heated at 120°C After heating for 30 min at room temperature and cooling to room temperature, the resulting white precipitate was washed three times with ethanol and dried. Next, the precursor was added to 40 mL ethanol solution containing 200 mg of thiourea and stirred for 2 min. The mixture was transferred to a 80 mL polytetrafluoroethylene Reaction in a stainless steel autoclave lined with vinyl fluoride at 180 °C for 3 h, after cooling to room temperature, the precipitate was washed three times with ethanol and ultrapure water and dried to obtain a yellow powder that is hollow multi-layer indium sulfide;

[...

Embodiment 2

[0042] Example 2 Preparation of Procalcitonin Secondary Antibody Incubation Solution Labeled with Gold-complexed Molybdenum Trioxide Hollow Nanospheres

[0043] (1) Preparation of molybdenum trioxide hollow nanospheres

[0044] First, 0.12 g C 10 h 14 MoO 6 Sonicate in 20 mL of n-butanol for 10 min to disperse the solid into the solvent evenly, then add 5 mL of 1 M nitric acid to the mixed solution under magnetic stirring and continue stirring for 1 h to transfer the obtained mixed solution to into a 50 mL polytetrafluoroethylene-lined stainless steel autoclave, after that, heat at 220 °C for 12 h, and cool to room temperature, wash the black precipitate twice with deionized water and absolute ethanol successively, at 60 °C The precursor MoO was obtained after drying for 5 h 2 , and then heated at 300 °C in air atmosphere for 2 h, the obtained white MoO 3 powder;

[0045] (2) Preparation of gold-composite molybdenum trioxide hollow nanospheres

[0046] 1 mL of 1% HAuCl ...

Embodiment 3

[0049] Example 3 Preparation of Procalcitonin Secondary Antibody Incubation Solution Labeled with Gold-complexed Molybdenum Trioxide Hollow Nanospheres

[0050] (1) Preparation of molybdenum trioxide hollow nanospheres

[0051] First, 0.13 g C 10 h 14 MoO 6 Sonicate in 30 mL of n-butanol for 10 min to disperse the solid into the solvent evenly, then add 10 mL of 1 M nitric acid to the mixed solution under magnetic stirring and continue stirring for 1 h to transfer the obtained mixed solution to into a 50 mL polytetrafluoroethylene-lined stainless steel autoclave, after that, heated at 220 °C for 12 h, and cooled to room temperature, and washed the black precipitate 6 times with deionized water and absolute ethanol in sequence, and at 60 °C The precursor MoO was obtained after drying for 5 h 2 , and then heated at 550 °C in air atmosphere for 5 h, the obtained white MoO 3 powder;

[0052] (2) Preparation of gold-composite molybdenum trioxide hollow nanospheres

[0053] 2...

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