The invention discloses a sensitive probe as well as a preparation method and a method for detecting 
salmonella enteritidis by virtue of the sensitive probe, relates to a probe, a preparation method and application of the probe, and aims to solve the problems of high cost, 
instability, high deactivation rate of an 
antibody and poor sensitivity of an existing probe. The sensitive probe is prepared from 
antibiotics and a 
signal carrier. The preparation method comprises the following steps: 1, preparing yellowish-brown 
emulsion; 2, preparing a carboxylated 
immunomagnetic bead; 3, preparing an activated magnetic 
microsphere; 4, adding a resuspension solution for storage. The method for detecting the 
salmonella enteritidis by virtue of the sensitive probe comprises the following steps: 1, preparing an 
immunochromatographic test strip for the 
salmonella enteritidis; 2, mixing liquid to be detected and the sensitive probe for incubation, adding dropwise a 
mixed solution onto a sample pad of the 
immunochromatographic test strip for the 
salmonella enteritidis, performing reaction for 10 minutes, and reading a result. According to the sensitive probe, the preparation method and the detection method, only one 
antibody is struck on a 
nitrocellulose membrane for direct detection, so that the cost is greatly reduced, and simplicity and convenience are achieved; the sensitive probe, the preparation method and the detection method are applied to detection of the 
salmonella enteritidis.