70results about How to "Wide application value" patented technology

The invention discloses a synthesis method of dicamba, which comprises the following steps of: using 2, 5-dichlorophenol as raw materials, conducting sulfonation and bromination reaction to obtain 4-hydroxy-2, 5-dichloro-3-bromobenzenesulfonic acid, treating the 4-hydroxy-2, 5-dichloro-3-bromobenzenesulfonic acid by using alkyl metal complexes of magnesium or lithium, conducting electrophilic addition reaction with CO2 to obtain 5-sulfo-3, 6-diclorsalicylic acid, removing the sulfo of the 5-sulfo-3, 6-diclorsalicylic acid and conducting O-methylation reaction with methyl chloride to obtain the dicamba. The synthesis method of dicamba has the advantages that since the 2, 5-dichlorophenol is used as the raw materials, the raw materials for reaction are easy to obtain, the reaction process is environmental-friendly, the quality of the obtained product is high, the yield is high and the application value is greater.

The invention discloses a helicopter taking off and landing vertically and flying horizontally and application thereof. The helicopter comprises a fuselage, rotor mechanisms, wing mechanisms, an undercarriage device, a power mechanism, a control mechanism and a transmission mechanism. The fuselage comprises a nose, a fuselage middle part and a tail. The rotor mechanisms comprise double rotating rotor mechanisms. Rotary double-rotor mechanisms are longitudinally arranged on the fuselage. The multiple wing mechanisms are perpendicularly arranged on the two sides of the fuselage middle part. As both the double rotating rotor mechanisms and the rotary double-rotor mechanisms are designed, both vertical taking-off and landing and rapid horizontal flying are achieved; as the rotor mechanisms are installed on the nose and the tail of the fuselage of the helicopter respectively and can rotate front and back around the center axis of the fuselage, the range of the helicopter is 5-6 times larger than that of an existing helicopter, flight safety is ensured, production and operation cost is reduced, the design can be applied to various aircrafts, and the significant technical effects of a large range, a high flight speed, a high safety coefficient and control easiness of the helicopter can be achieved.

The balance has the space-parallel closed loop structure formed by connecting up platform of main bearing rod and down platform set on wind-tunnel with ball-joint set on periphery of up and down platforms through connecting rod set on with force sensing element, of which the connecting rod is formed by connecting the up connecting rod and the down connecting rod with nuts.

The invention relates to a quadruped robot control method and control device on the basis of a sine opposite angle gait and a quick table look-up method. The method comprises the following steps that: (1) combining with the opposite angle gait to analyze the leg structure of a quadruped robot, establishing the leg movement mathematical model of the quadruped robot, and adopting a novel sine gait; (2) solving a nonlinear equation set to obtain the control rate of each joint of four limbs, establishing a database which specially faces robot movement control, obtaining an accurate joint opening and closing angle through the quick table look-up method, and quickly and accurately realizing foot end trajectory control; and (3) designing a foot end trajectory control unit and a steering control unit so as to bring convenience to the access of other control systems. On an aspect of trafficability characteristics, the novel sine gait is designed and adopted, and therefore, and the robot owns high trafficability when the robot faces a complex terrain. On the aspect of operation stability, when a step taking period stops, the horizontal speed of a swing phase is zero, and therefore, the method is high in stability. The table look-up method is adopted to quickly, accurately and stably carry out the foot end trajectory control.

The invention discloses a surface modified silicon dioxide material, a preparation method and application thereof and belongs to the technical field of a compound material. The method provided by the invention comprises the following steps of: adding nanometer silicon dioxide into ethyl alcohol, and then adding 3-(2,3-epoxy propoxy) propyl trimethoxy silane and performing backflow reaction, thereby acquiring GPTMS-SiO2; putting GPTMS-SiO2 into ethyl alcohol, adding polymine and performing backflow reaction, thereby acquiring PEI-SiO2; and putting the acquired PEI-SiO2 into distilled water, and then adding sodium 2-bromoethanesulphonate and sodium hydroxide, performing backflow reaction at 70 DEG C, centrifugally separating and drying, thereby acquiring the surface modified nanometer silicon dioxide. The nanometer material prepared according to the invention can selectively absorb silver ions in aqueous solution and can be widely applied to the recycling treatment of silver ions in industrial waste water.

The invention discloses a fast image defogging method based on dark channel prior and a Retinex theory. The method comprises the following steps of: firstly obtaining a simplified atmospheric scattering model; then obtaining an initial estimation value and a rough estimation value of a medium transmission rate; carrying out pixel-stage fusion to obtain a final estimation value of the medium transmission rate; and finally substituting the final estimation value of the medium transmission rate into the simplified atmospheric scattering model for image restoration to obtain a defogged image. The method provided by the invention has the advantages that a higher operation speed is realized; the definition and the contrast ratio of a restored image can be effectively improved; meanwhile, better image colors are obtained; and wide prospects and great application values are realized in the fields of urban traffic, video monitoring, intelligent vehicles and the like.

The invention relates to the technical field of marine chemical engineering, in particular to chitosan quaternary ammonium salt containing organic acid salt as well as a preparation method and application thereof. Chitosan, 3-chloro-2-hydroxypropyltrimethyl ammonium chloride, sodium hydroxide, isopropanol, gallic acid, caffeic acid and ascorbic acid are used as raw materials; firstly, the chitosanis dispersed in isopropanol for swelling expansion; at room temperature stirring is performed for 4h; 3-chloro-2-hydroxypropyltrimethyl ammonium chloride and a sodium hydroxide solution are added; continuous reaction is performed for 12h; then, a proper amount of ethyl alcohol is used for precipitation; the chitosan quaternary ammonium salt is obtained; finally, the chitosan quaternary ammonium salt is dissolved in water; ion exchange with the organic acid salt is performed; a final product is obtained. The chitosan quaternary ammonium salt has the advantages that active groups in derivativesare added; the activity is high; the preparation process is simple; side reaction is little; the cost of the used materials is low. The research proves that the derivatives have good water solubility; the antioxidant activity is high; wide application values are realized in the industry of medicine and functional food health care, particularly cosmetics industry.

The invention discloses a group of microsporidium molecule universal detection primers and a kit thereof. The universal detection primers include an upstream primer HMG1F and a downstream primer HMG1R; the nucleotide sequence of the upstream primer is as shown in SEQ ID NO.3, and the nucleotide sequence of the downstream primer is as shown in SEQ ID NO.4. The detection primers target at the gene HMG1 and can be applied to detecting a plurality of microsporidia simultaneously, and the detection results are reliable, easy to operate and high in sensitivity; especially, the universal detection primers have extremely important practical application significance to early detection of various microsporidia in a sample in practical quarantine work.

This invention relates to a material with manganic acid lanthanum film and heterojunction on the silicon sheet and its preparing method. The material comprises a layer of P type doping manganic acid lanthanum La1-xAxMnO3 film on the n type silicon sheet to form p-n heterojunction material; wherein, A is Ca, Sr, Ba, Pb or Sn, any x ranges from 0.05 to 0.5; or a layer of n type doping manganic acid lanthanum La1-xBxMnO3 film on the p type silicon sheet to form p-n type heterojunction, wherein, B is Ce, Pr, Te, Nb, Sb or Ta, any x ranges from 0.05 to 0.5. The preparing method adopts two steps: epitaxial growing the manganic acid lanthanum or doped manganic acid lanthanum material on the silicon bulk, or used as breaker, then, epitaxial grow other perovskite oxide compound film, or multiple films.

The invention relates to a method for controlling the flowing of magnetic liquid. The method comprises the following steps that a, magnetic field generating devices are symmetrically arranged at the two sides of a passage container, and the magnetic liquid is contained in the passage container; b, the magnetic field generating devices generate a gradient magnetic field, the gradient direction of the gradient magnetic field is regulated, and when the gradient direction of the gradient magnetic field generated by the magnetic field generating devices is identical to the magnetic liquid flowing direction, the flowing speed of magnetic nanometer particles in the magnetic liquid is accelerated under the magnetic force effect; when the gradient direction of the gradient magnetic field generated by the magnetic field generating devices is opposite to the magnetic liquid flowing direction, the flowing speed of the magnetic nanometer particles in the magnetic liquid is decelerated under the magnetic force effect; and the goal of controlling the flowing of the magnetic liquid is further achieved through regulating the dimension of the magnetic field gradient. The method has the advantages that the structure is novel, the method is unique, and wide scientific and application values are realized in the fields of heat exchangers, cooling loops and energy conversion.

The invention discloses a heat radiation layer with a phase transition material, a preparation method of the heat transition material and a photovoltaic assembly with the heat transition material. Theheat radiation layer is composed of an internal phase transition composite material and a wrapping material which wraps the phase transition composite material, the phase transition composite material is formed by combining a phase transition material and a support material in a dipping adsorption method, and the phase transition material occupies 80wt%-90wt% of the whole heat radiation layer. The heat radiation layer uses the phase transition layer to release or absorb heat from the environment and thus release or store energy in the phase transition process. The photovoltaic assembly with the heat radiation layer can balance heat generated in the working process of the photovoltaic assembly, power loss caused by too high working temperature of the assembly can be reduced, and thus, thepower generation power of the photovoltaic assembly is improved effectively, and the power generation cost is reduced.

The present invention relates to a forest fire early warning system, comprising a monitoring center and several monitoring terminals, the monitoring center comprising a data acquisition module, a data analysis module, and an early warning module, and the monitoring terminal comprising a terminal controller and an infrared camera connected to the controller , a temperature and humidity sensor, a smoke sensor and a wireless communication module, the wireless communication module realizes information interaction with the monitoring center; it also includes a solar power supply system; the present invention monitors the state of the forest in real time through a monitoring terminal infrared camera, a temperature and humidity sensor and a smoke sensor, and Wirelessly transmit the data to the monitoring center for analysis and processing, make corresponding emergency measures and distribution and scheduling of fire extinguishing methods, and use solar energy to power the forest fire prevention early warning system, improve energy utilization, high degree of intelligence, easy operation and reliability High, with a wide range of promotional significance and application value.

The invention provides a fully automatically mechanical generator, which applies the principle of the elastic device of a mechanical watch to the invention, meanwhile, the invention is provided with a variable speed device; the end of the variable speed device is combined with the generator; furthermore, the invention is provided with a rotation speed controller and an exterior energy filling part; the controller releases the stored mechanical energy by a certain rate, thus driving the generator to generate power; the energy filling means that the released mechanical energy is supplemented according to the instruction of a computer control center when the stored mechanical energy is released to a certain degree; the automatic generator has the advantages of no requirement of external energy source, 100% environmental protection, being able to be applied to all electric fields, bringing an energy-saving revolution for the automobile industry, and being able to be applied to all field or movable electric industries such as ships, planes, trains, military affairs, national defense, etc. without any restriction of the weather conditions.

The invention relates to an organic-silicon water-repellent agent. Every liter of finishing agent comprises the following components in mass by weight: 25-80g of self-crosslinking organic-silicon finishing agent, 7-20g of organic-silicon crosslinking agent, 0.01-0.1g of alkaline and the balance of deionized water. The organic-silicon water repellent agent is mainly used for durable and water-repellent finishing of cotton, polyester, nylon and blended fabrics in finishing after printing and dyeing, and simultaneously endows the fabrics with good resilience and rich handfeel; and since the organic-silicon finishing agent does not contain fluorine and a solvent, no hazard is caused to a human body and the environment, and the properties in the process of storage and transportation keep stable. The organic-silicon water-repellent agent is a green and environment-friendly water-repellent agent, thus having wide application value and prospect in the industry, especially in the aspect of water-repellent finishing of textile products.

The invention discloses a method for separation, enrichment and detection of Cu and Zn in food. The method is as below: co-precipitating Cu and Zn in a food sample with 2,9 -dimethyl-4,7-diphenyl-1,10- phenanthroline disodium sulfonic acid (BCP); and then determining the content of Cu and Zn by using a flame atomic absorption spectrometry (FAAS). The method has the advantages of simple process, high detection and sedimentation speed and low cost; BCP is used as a co-precipitation agent, and no vector element is needed. Compared with the prior literature art, the method has the advantages of low detection limit and high degree of accuracy.

The invention discloses a method for activating gene expression by a CRISPR-assisted trans-enhancer. The method is characterized in that an enhancer DNA is collected onto a target gene in a trans manner by virtue of a CRISPR system, and the target gene expression is activated by virtue of the trans enhancer DNA and the interaction of the trans enhancer DNA and CRISPR system. By improving the conventional guidance RNA(sgRNA), a capturing sgRNA(csgRNA) is developed; and the CMV enhancer is collected onto the target gene in a trans manner by virtue of the hybridization of csgRNA in a dCas9-AD / csgRNA complex and enhancer DNA with CMV, so that the expression of the target gene can be efficiently activated. The method has wide application value in the biological medicine, and can be applied to the preparation of biological detection and treatment reagents.

Belonging to the field of inorganic nonmetal negative thermal expansion functional materials, the invention discloses a negative thermal expansion ceramic material ScxIn2-xW3O12 and a preparation method thereof, wherein x is greater than or equal to 1 and smaller than or equal to 1.2. The negative thermal expansion ScxIn2-xW3O12 ceramic material adopts Sc2O3, In2O3 and WO3 as the raw materials and is prepared by solid phase method. The preparation method includes: weighing raw material oxides according to certain molar ratio, and conducting ball milling, molding and sintering at 950-1200DEG C to obtain the negative thermal expansion ceramic material ScxIn2-xW3O12 with compact structure and stable negative thermal expansion performance at a temperature ranging from room temperature to a melting temperature. For example, in a temperature range from room temperature to 700DEG C, ScInW3O12 ceramic has a linear thermal expansion coefficient of -5.97*10<-6> / K, an almost linear thermal expansion curve, no phase change, stable thermal expansion performance, and good negative thermal expansion performance. At the same time, the preparation method of the negative thermal expansion material ScxIn2-xW3O12 provided by the invention has the advantages of simplicity, short preparation cycle, low cost, environmental protection and no pollution, thus having good application prospect.

The invention relates to a nano-amplified self-reference probe for in-situ imaging of microRNA (miRNA) in living cells and a preparation method thereof. The probe is formed by encapsulating a nucleicacid hairpin modified upper conversion nanoparticle (UNP-H1) and a fluorescent molecule modified hairpin molecule (H2-F) through a liposome. When the probe enters a cell, UNP-H1 and H2-F are released,and only when H1 is hybridized to the miRNA, H2 can replace the miRNA to hybridize with UNP-H1, and the released miRNA starts the next cycle to amplify a signal. Under excitation of near-infrared light, luminescence resonance energy transfer (LRET) occurs between the emission band 1 (EEB1) and F of the UNP. The luminescence intensity ratio of the LRET and EEB2 channels (ILRET / IEEB2) can be used for relative quantification of intracellular miRNA taking emission band 2 (EEB2) channel of the UNP as a self-reference.

The invention relates to a method for controlling size of boiling liquid bubbles. The method specifically includes that (1) when a magnetic field generating device generates a gradient magnetic field of which a gradient direction and a gravity direction are the same, a magnetic force is exerted on boiling liquid, the direction of the magnetic force and the gravity direction are the same, at the moment, bubble desorption size is reduced due to combined action of a resultant force, bubbles are rapidly subjected to desorption from a heating surface, and the boiling heat transmission efficiency is increased; the bubble desorption size is smaller and the boiling heat transmission efficiency is higher with the increasing of gradient of the gradient magnetic field; (2) when the magnetic field generating device generates a gradient magnetic field of which the gradient direction and the gravity direction are opposite, a magnetic force is exerted on the boiling liquid, the direction of the magnetic force and the gravity direction are opposite, at the moment, bubble desorption size is increased due to combined action of the resultant force, desorption of the bubbles from the heating surface slows down, and the boiling heat transmission efficiency is reduced; the bubble desorption size is larger and the boiling heat transmission efficiency is lower with the increasing of gradient of the gradient magnetic field. According to the method for controlling size of the boiling liquid bubbles, wide scientific and applicable values can be achieved in terms of fields of heat exchangers, cooling loops and energy conversion.

The invention discloses a preparation method of a biological activated carbon filter. The method comprises a test process. The test process comprises pre-ozonation and coagulation, settling, filteringand ozone-biological activated carbon, wherein conventional processing parameters are basically constant, that is, processing water volume is 3-3.5m<3> / h, mixing time is 6-6.5s, reaction time is 23.2-19.9min, an ascending flow rate of a settling tank clear water area is 1.39-1.62mm / s, an ascending flow rate in an oblique tube is 1.60-1.87mm / s, a filter rate of the filter is 6.49-7.57m / h, a coagulant and a pH regulating agent are respectively liquid alkaline aluminum and sodium hydroxide, feeding concentrations are respectively around 2.5mg / L and 6mg / L, and then, the biological activated carbon filter is obtained. A detection method of the filter involves ammonifying bacteria, nitrite bacteria, nitrifying bacteria, heterotrophic bacteria, and dehydrogenase activity. The preparation methodhas the advantages of eliminating organic matters and polluted matters in feed water of a carbon filter of a water supply factory and purifying the water. Compared with other sewage deep processing technologies, the biological activated carbon filter prepared by the method has a simple structure, occupies a small space, and runs conveniently.

The invention provides a paste prepared from grape, ginseng fruit and honey, which is prepared from the following raw materials: grape, ginseng fruit, red bean, cashew nut, cantaloupe, garland chrysanthemum, nutrient solution, a proper amount of pure water, honey, walnut oil and spirulina powder. The preparation method includes the following steps: (1) preparing a mixed fruit pulp; (2) preparing a cereal grain pulp; (3) preparing a fruit and vegetable juice; and (4) preparing a nutrient solution; and (5) mixing the mixed fruit pulp, the cereal grain pulp, the fruit and vegetable juice and the nutrient solution, adding walnut oil, spirulina powder and honey, and blending into a paste to obtain a finished product. The paste provided by the invention integrates honey, fruits and vegetables, cereal grains and nourishing herbs scientifically, is rich in taste, can fully supplement the nutrients needed by the human body, and has the health-care effects of enhancing human immunity, tonifying middle-Jiao, replenishing qi, removing toxic materials, caring skin, resisting aging, lowering blood pressure and the like.

The invention relates to a quick-start interactive relationship labeling and extracting framework. The quick-start interactive relationship labeling and extracting framework comprises the following steps: S1, pre-training a named entity recognition model by using a general named entity recognition data set; s2, pre-training a few-sample relation extraction model by using a general relation extraction data set; s3, setting a to-be-extracted relationship and a small amount of annotated data; s4, performing data preprocessing on the to-be-extracted text; s5, performing named entity recognition on the to-be-extracted text by using the named entity recognition model; s6, carrying out manual pairing on the entities; s7, performing preliminary relation extraction on a pairing result; s8, performing manual proofreading on a relation extraction result; s9, performing fine adjustment on the few-sample relation extraction model; and S10, repeating the steps S4 to S9 until all the texts to be extracted are processed. According to the scheme, the defects of high starting cost and heavy labor cost investment in the prior art are overcome, and relationship labeling and extraction with the characteristics of quick starting and low labor cost are realized.

The invention discloses a Hibiscus rosa-sinensis Linn cutting medium and a Hibiscus rosa-sinensis Linn cutting method under a high-temperature condition. The cutting medium is prepared by uniformly mixing the following components in parts by volume: 10-60 parts of urban landscaping waste compost, 0-50 parts of peat and 40-50 parts of perlite. On the one hand, the urban landscaping waste compost decomposed by a composite fermentation microbial agent is added into the medium, and multiple functional probiotic floras are introduced into the cutting medium and fight with residual pathogenic bacteria in soil for nutrition and various survival conditions by utilizing symbiotic cooperation or synergism of multiple microorganisms in the probiotic floras, so that soil-borne diseases are competitively inhibited; meanwhile, growth of the pathogenic bacteria causing root rot is inhibited through antagonism among the microorganisms, rot of cutting slips is reduced, and survival of the cutting slipsis improved; and on the other hand, the characteristics of water retention and fertility of the urban landscaping waste compost are utilized, growth of plant root systems can still be promoted underthe high-temperature condition, survival and later-period growing power of cutting seedlings are guaranteed, and the cultivation time and period of the cutting seedlings are shortened.

The invention relates to the technical field of equipment maintenance support, and discloses a new research equipment spare part variety determination method. Through fuzzy rough definition and a spare part variety determination model of hesitant fuzzy rough set decision, a hesitant fuzzy decision information system of spare part variety determination, a numerical continuation method of risk preference coefficients, and attribute reduction and rule acquisition of improved inclusion degree calculation are constructed. The method comprises the following specific steps: calculating selection of all decision-making conditions, obtaining hesitant fuzzy decision-making information after condition attributes are recombined, calculating the inclusion degree of the condition attributes in the decision-making attributes in a hesitant fuzzy decision-making information table after the combination, deleting condition attributes item by item, calculating a new decision rule, if the new decision rule is not changed, removing the deleted condition attributes redundantly, and repeating calculation, so a maximum decision reduction set is obtained. The invention provides an extraction method for determining the variety of spare parts. The problem of decision information application under the condition of incomplete information is solved. The effect of the method is verified, and the method has wide application value and application scenes.

The invention belongs to the technical field of inorganic non-metallic materials, particularly relates to a nano barium titanate material with a low K value and a high refractive index, and further discloses a preparation method of the nano barium titanate material and nano barium titanate dispersion liquid prepared from the nano barium titanate material. According to the invention, in the nano barium titanate material, proper organic alkali is selected as a template agent in a hydrothermal synthesis process; the mesoporous structure, the crystal form and the grain size of barium titanate arecontrolled by adjusting the content of a template agent and hydrothermal synthesis conditions, and then the ultrafine mesoporous cubic-phase nano barium titanate suitable for the mesoporous structure,parameters and low K value is obtained through crystallization. The dielectric constant K value of the nano barium titanate is only 49-82 and is obviously smaller than the dielectric constant k = 100-200 of nano barium titanate obtained through conventional hydrothermal synthesis, so that the nano barium titanate has wider application fields and higher application value.

Methods and apparatuses for fast error detection and correction of computer data for resilient communication and storage; for encoding and recovery of data with some communicated or stored data parts lost; and for controlling access to data in a decentralized data storage system.

The invention provides a true color-based massive point clouds dynamic display method, solving the defects of the prior art. The method comprises a first step of acquiring true color-based massive point cloud data, and performing optimization and reconstruction on the acquired point cloud data, wherein the optimization and reconstruction method comprises steps of first, performing orientation and parameter marking on the point cloud data by using a normal vector; then performing numerical reduction optimization on the massive point cloud data; and finally, forming a dynamic display marked data package of massive point clouds with true color; a second step of adjusting a data model according to access information of users, and displaying corresponding display information; and a third step of optimizing display data in the display process. In addition, the invention provides a true color-based massive point clouds dynamic display system. The system comprises an access information receiving module, a data processing module, a data storage module, and a display module. The feedback speed of the display image is faster than that in the prior art by 50% or more.

The invention discloses a kind of screening method for barley re-cadmium-poison genotype, comprises selecting seed, disinfecting, culturing seedling, transplanting, pre-culturing, cadmium coerion treatment, re-cadmium-poison evaluation; cadmium treat 20 days, detect value of the chlorophyll device for the middle of the functional leave; the decline ratio(%)= 100X(20Umcd treatment detection value - concrete with detection value) / concrete with detection value ; the integration score of each breed= value of the chlorophyll device declined ratioX0.5+ root volume declined ratioX0.1+root number declined ratioX0.1+ dry weight of underground declined ratioX0.1+stalk high declined ratioX0.1.

The invention discloses a current sensor based on a magneto-optic nonreciprocal MZ interference structure. The current sensor is based on an MZ interference structure, and is formed in a way that two interference arms are respectively connected with a beam splitter and a beam combiner, wherein magneto-optic material covering layers are respectively embedded in the two bending optical fiber interference arms, and the embedded positions of the two magneto-optic material covering layers should ensure that the light transmission directions are opposite to each other in parallel. By introducing the nonreciprocal phase shift to the MZ structure, the phase difference generated in the transmission of the light in the two optical fiber interference arms works in a nonreciprocal push-pull mode, and the reciprocal phase shift amount generated by ambient environment factors and the like (such as temperature, stress and the like) is counteracted by the MZ interference structure. By using the nonreciprocal characteristics of the magneto-optic material, combining the interference characteristic of the MZ structure and optimizing the structure parameters, the invention can realize the current detection of high stability and high sensitivity. The invention has the characteristics of popular structure, simple technology, flexible design, strong functionality and the like, and has extensive application prospects and application values in the aspect of current sensing in the complex environment.

The invention discloses a primer, a plasmid and a method for the detection of mulberry mosaic type atrophy-associated viruses. The primer comprises an upstream primer MCP746f and a downstream primer MCP1148r, and nucleotide sequences of the upstream primer and the downstream primer are respectively shown as SEQ ID NO.1 and SEQ ID NO.2; meanwhile, the positive plasmid for detection is constructed,the fragment construction plasmid 13MCP-pEASY-Bblunt with a size of 1309 bp is obtained by the amplification of an upstream primer 13MCP156f and a downstream primer 13MCP1464r, and nucleotide sequences of the plasmid and the primers are respectively shown as SEQ ID NO.3, SEQ ID NO.4 and SEQ ID NO.5. The primer disclosed by the invention can specifically detect the mulberry mosaic type atrophy-associated viruses, is reliable in detection results, easy to operate, strong in specificity and high in sensitivity, particularly has an important practical application significance in the detection of diseased leaves at the early stage of infection (during the latent incubation period), meanwhile can detect pathogenic bacteria existing on leaves, petioles, buds and branches of diseased plants and has a practical guiding significance in the prevention and the control of the mulberry mosaic atrophy-associated viruses.