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Red crucian, Japanese white crucian and megalobrame amblycephala subfamily distant hydridization method

A technology of distant hybridization and Tuantou bream, which is applied in fish farming, application, climate change adaptation, etc., can solve the problems of no crucian carp and Tuantou bream subfamily, and many difficulties in distant hybridization.

Active Publication Date: 2008-04-23
HUNAN NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Red crucian carp (Carassius auratus red var.) is a diploid fish (2n=100) belonging to Cyprinidae and Cyprinidae, and Japanese white crucian carp (Carassius auratus cuvieri) is a diploid fish ( 2n=100), the group head bream (Megalobrama amblycephala) is a diploid fish (2n=48) belonging to Cyprinidae and the subfamily Cyprinidae; in terms of biological characteristics such as genetics, classification and body shape, red crucian carp, Japanese white crucian carp and There is a big difference between the group bream, especially the number of somatic chromosomes between them is very different, the number of chromosomes of red crucian carp and Japanese white crucian carp is 100, while the number of chromosomes of group head bream is 48, and they are not multiplied Sexual relationship, so distant hybridization is more difficult
Up to now, there has been no report on the successful distant hybridization between crucian carp and bream subfamily at home and abroad

Method used

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  • Red crucian, Japanese white crucian and megalobrame amblycephala subfamily distant hydridization method
  • Red crucian, Japanese white crucian and megalobrame amblycephala subfamily distant hydridization method
  • Red crucian, Japanese white crucian and megalobrame amblycephala subfamily distant hydridization method

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Embodiment Construction

[0011] The key of the present invention lies in the selection of parents, with red crucian carp and Japanese white crucian carp as the female parent, and roundhead bream as the male parent, the specific method is: select sexually mature female red crucian carp and white crucian carp, and squeeze out their mature eggs , respectively mixed with the sperm of bream for artificial dry insemination, and the sperm and eggs were fully stirred with clean chicken feathers. The fertilized eggs are hatched in a petri dish, the water temperature is 20-21°C, and the hatched fry are raised in a pond. The number of chromosomes in somatic cells of the offspring of red crucian carp (♀)×Plughead bream (♂) and white crucian carp (♀)×Plughead bream (♂) was detected by direct preparation of kidney cells. The method of direct production of kidney cells is as follows: After cultivating the experimental fish for 1-3 days at a water temperature of 18-22°C, inject PHA to the experimental fish 1-3 times,...

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Abstract

The present invention relates to distant hybridization technique of fishes. Said invention selects red crusian carp and Japanese white crusian carp as female parent, and uses triangular bream as male parent, and adopts artificial dry method to make insemination, said fertilized egg is undergone the process of incubation treatment to obtain filial generation of triploid and tetraploid. Said invention not only can improve character, but also is a new way for producing triploid and tetraploid.

Description

Technical field: [0001] The invention relates to a fish hybridization method, in particular to a fish distant hybridization technique. Background technique: [0002] Hybridization technology is a commonly used method to improve fish traits. Generally, hybridization between species or above species is called distant hybridization. There have been some reports on distant hybridization of fish at home and abroad, such as bighead carp (♀) × silver carp (♂), grass carp (♀) × silver carp (♂), red crucian carp (♀) × carp (♂), etc. The two are hybrids between subfamilies. Through distant hybridization, the traits of fish can be improved. For example, the hybrid offspring formed by the intergeneric distant hybridization of red crucian carp and Xiangjiang wild carp has the advantages of fast growth, fresh and tender meat, strong disease resistance, and high survival rate. Red crucian carp (Carassius auratus red var.) is a diploid fish (2n=100) belonging to Cyprinidae and Cyprinidae,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 刘少军刘筠罗凯坤孙远东张纯申佳珉
Owner HUNAN NORMAL UNIVERSITY
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