Red crucian, Japanese white crucian and megalobrame amblycephala subfamily distant hydridization method
A technology of distant hybridization and Tuantou bream, which is applied in fish farming, application, climate change adaptation, etc., can solve the problems of no crucian carp and Tuantou bream subfamily, and many difficulties in distant hybridization.
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[0011] The key of the present invention lies in the selection of parents, with red crucian carp and Japanese white crucian carp as the female parent, and roundhead bream as the male parent, the specific method is: select sexually mature female red crucian carp and white crucian carp, and squeeze out their mature eggs , respectively mixed with the sperm of bream for artificial dry insemination, and the sperm and eggs were fully stirred with clean chicken feathers. The fertilized eggs are hatched in a petri dish, the water temperature is 20-21°C, and the hatched fry are raised in a pond. The number of chromosomes in somatic cells of the offspring of red crucian carp (♀)×Plughead bream (♂) and white crucian carp (♀)×Plughead bream (♂) was detected by direct preparation of kidney cells. The method of direct production of kidney cells is as follows: After cultivating the experimental fish for 1-3 days at a water temperature of 18-22°C, inject PHA to the experimental fish 1-3 times,...
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