Method for separating and purify dermatansulfate and low-molecular heparan sulfate from sodium heparan product

Abstract

The invention relates to a method to separate and purify dermatan sulfate and low molecule heparan sulfate from sodium heparin by-product. It uses the by-product of producing sodium heparin as raw material taking fractional precipitation through alcohol to gain raw dermatan sulfate; uses nitrite and nitrous acid ester compounding as oxidant to degrade the heparan sulfate into low molecule heparan sulfate and taking fractional precipitation through alcohol to separate high purity dermatan sulfate and low molecule heparin sulfate.

Description

technical field

[0001] The invention relates to a method for separating and purifying dermatan sulfate and low molecular weight heparan sulfate from by-products of heparin sodium, and belongs to the field of mucopolysaccharide biopharmaceuticals. Background technique

[0002] Dermatan sulfate is a mucopolysaccharide, usually mixed with other mucopolysaccharides in animal tissues. Dermatan sulfate is composed of (1-4)-iduronic acid-(1-3)-acetylamino-2-deoxy-4-O-sulfate-β-D-galactobiose basic unit, optical rotation-55 ~-63 degrees, anticoagulant potency ≤10USP u / mg, ratio of sulfate group to carboxylic acid group is 0.8~1.2.

[0003] Pure dermatan sulfate has low anticoagulant potency, high activity of activated heparin cofactor II, and no bleeding side effects, so it has a good prospect as an antithrombotic drug.

[0004] Heparan sulfate is a subcomponent of heparin, which exists in unseparated heparin and is called fast-moving heparin. Compared with slow-moving heparin, he...