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Method for measuring sludge dehydrogenase activity

A technology of activity measurement and dehydrogenase, which is applied in the direction of color/spectral characteristic measurement, preparation of test samples, and analysis by making materials undergo chemical reactions, can solve problems such as rapid fading, and avoid extremely volatile, production Simple steps, enhanced stability effect

Inactive Publication Date: 2009-05-20
UNIV OF SCI & TECH OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a reliable method for measuring sludge dehydrogenase activity, to overcome the shortcomings of rapid fading of TF in the preparation of standard curves and the need for high-temperature extraction in sample testing, thereby improving the accuracy of sludge dehydrogenase activity determination

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1: measure the dehydrogenase enzyme activity of activated sludge

[0015] 1. Sample preparation: take 45ml of activated sludge (taken from a sequencing batch reactor in the laboratory, sludge concentration MLVSS=3g, influent COD=1000mg / L, DO=3mg / L, operation cycle=4h, enter Water = 10min, aeration = 215min; sedimentation = 5min; effluent = 10min) in a 150ml Erlenmeyer flask, shake vigorously with glass beads for 5 minutes to break up the sludge, discard the supernatant after centrifugation, then make up with distilled water, and stir Wash, repeat three times. Then dilute to the original volume with distilled water and stir with a glass rod to suspend the sludge for later use.

[0016] 2. Standard curve creation

[0017] ① Accurately weigh 100mg of dried TTC into a 100ml volumetric flask, and make a 1mg / ml TTC solution at constant volume.

[0018] ②Put 1.0, 2.0, 3.0, 4.0, and 5.0 ml of TTC solution into five 50 ml volumetric flasks in turn, and constant vol...

Embodiment 2

[0033] Embodiment 2: Measuring the activity of methanogenic sludge dehydrogenase

[0034] 1. Sample preparation: Take 20ml of methanogenic sludge (taken from a methanogenic UASB reactor, sludge concentration MLSS=10g, influent COD=10000mg / L, HRT=10h) in a 150ml Erlenmeyer flask, and add 20ml Dilute with water, shake vigorously with glass beads for 5 minutes to break up the sludge, discard the supernatant after centrifugation, make up with distilled water, stir and wash, repeat three times. Then dilute to the original volume with distilled water and stir with a glass rod to suspend the sludge for later use.

[0035] 2. Standard curve creation

[0036] ① Accurately weigh 50mg of dried TTC into a 50ml volumetric flask, and make a 1mg / ml TTC solution at constant volume.

[0037] ②Put 1.0, 3.0, and 5.0 ml of TTC solution into three 50 ml volumetric flasks in turn, and add the solutions with concentrations of 20, 60, and 100 mg / L to the volume.

[0038] ③Take three 20ml sample tube...

Embodiment 3

[0052] Example 3: Measuring the dehydrogenase enzyme activity of hydrogen-producing sludge

[0053] 1. Sample preparation: Take 35ml of hydrogen-producing sludge (taken from a hydrogen-producing UASB reactor, sludge concentration MLSS=6.3g, influent COD=5000mg / L, HRT=18h) in a 250ml Erlenmeyer flask, and add Dilute with 30ml of water, shake vigorously with glass beads for 5 minutes to break up the sludge, discard the supernatant after centrifugation, make up with distilled water, stir and wash, repeat three times. Then dilute to the original volume with distilled water and stir with a glass rod to suspend the sludge for later use.

[0054] 2. Standard curve creation

[0055] ① Accurately weigh 100mg of dried TTC in a 100ml volumetric flask, and make up to 1mg / ml TTC standard solution.

[0056] ②Put 1.0, 2.0, 3.0, 4.0, 5.0, and 6.0 ml of TTC standard solution into six 50 ml volumetric flasks in turn, and add the concentrations to 20, 40, 60, 80, 100, and 120 mg / liters of so...

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Abstract

The present invention belongs to the field of microbe activity measuring technology, and is especially the measuring method of sludge dehydrogenase activity for biological waste water treatment. The measuring method includes three work processes of conventional sample preparation, standard curve making and sample measurement. In standard curve making process, the reductant si 10-20 % concentration Na2S.9H2O solution; and in sample measurement process, mixed liquid compounded in conventional method is first cultured, then separated and added with acetone to extract TF, and finally mixed and color compared. Compared with available technology, the present invention has simple standard curve making, raised TF stability and accuracy, high reliability, and other advantages.

Description

Technical field: [0001] The invention belongs to the technical field of microbial activity measurement, in particular to a method for measuring sludge dehydrogenase activity in wastewater biological treatment process. Background technique: [0002] In the process of wastewater biological treatment, since dehydrogenase activity can effectively reflect the amount of active microorganisms contained in the sludge in the treatment system and its degradation activity on organic matter, the determination of dehydrogenase activity has become a very effective method for monitoring wastewater biological treatment. . [0003] Usually, the determination of sludge dehydrogenase activity is designed according to the mechanism of microbial oxidative respiration. That is, TTC is used as the final hydrogen acceptor, and no exogenous substrate is added to detect the metabolic activity of endogenous respiration. When endogenous metabolism occurs, TTC is transformed into red TF by dehydrogenat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31G01N21/78G01N1/34
Inventor 俞汉青郑煜铭赵全保
Owner UNIV OF SCI & TECH OF CHINA
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