Solid phase method for extracting elution reagent by using derivative of adamantine as supporter containing cyclodextrin of solid phase extraction

A solid-phase extraction column, adamantane technology, which is applied in the field of solid-phase extraction technology for separation and enrichment of chemical and chemical operations, can solve problems such as inability to elute, and achieve the effect of rapid detection

Inactive Publication Date: 2009-09-02
SHANGHAI UNIV
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  • Description
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Problems solved by technology

Due to the inclusion effect of cyclodextrin, some compounds cannot be effectively eluted by common organic elution solvents such as methanol and acetonitrile

Method used

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  • Solid phase method for extracting elution reagent by using derivative of adamantine as supporter containing cyclodextrin of solid phase extraction
  • Solid phase method for extracting elution reagent by using derivative of adamantine as supporter containing cyclodextrin of solid phase extraction
  • Solid phase method for extracting elution reagent by using derivative of adamantine as supporter containing cyclodextrin of solid phase extraction

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Embodiment 1

[0041] The instruments and experimental conditions adopted in this embodiment are as follows:

[0042] (1) Capillary electrophoresis instrument is a high-efficiency capillary electrophoresis system (CE-L1) produced by Singapore Pte Company, equipped with a UVIS 200 ultraviolet detector, and data acquisition and processing are completed by CSW chromatographic software.

[0043] (2) Micellar Electrokinetic Chromatography (MEKC) process, using the above capillary electrophoresis instrument. 20 mM SDS was prepared with 25 mM phosphate buffer (pH 8.0), then 5% acetonitrile was added, and the solution was filtered through a filter with a 0.45 micron pore size to obtain a MECK buffer solution. The pressure injection method (0.3psi) was adopted, the injection time was 10s, the separation voltage was 20kV, the detection wavelength was 214nm, and the experimental temperature was 25°C.

[0044] (3) β-cyclodextrin (β-CD), purchased from Sigma, USA; β-cyclodextrin bonded silica gel (CDS) ...

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Abstract

The invention relates to a solid-phase extraction method using an adamantane derivative as an eluting reagent component containing a cyclodextrin solid-phase extraction carrier, and belongs to the technical field of solid-phase extraction technology related to separation and enrichment chemistry and chemical operation. The steps of the method of the present invention are as follows: (1) activation of the solid-phase extraction column containing the cyclodextrin support, washing the extraction column with methanol or twice distilled water; (2) loading the sample, adding the sample solution to the solid-phase extraction column, And force the solution to pass through the stationary phase, and keep the sample analyte on the stationary phase; (3) rinse, rinse with twice distilled water, etc., to wash off unnecessary sample components or omit this step; (4) elute , passing the elution reagent containing adamantane derivatives through the solid-phase extraction column, so that the analyte is eluted from the cyclodextrin solid-phase extraction support; (5) detection and analysis, the eluent collected above is directly carried out Chromatography, electrophoresis, etc., and calculate and analyze the obtained detection curve, or collect the eluate for other research work.

Description

technical field [0001] The invention relates to a solid-phase extraction method using an adamantane derivative as an elution reagent containing a cyclodextrin solid-phase extraction carrier, and belongs to the technical field of solid-phase extraction technology related to separation and enrichment chemistry and chemical operation. Background technique [0002] Solid phase extraction (SPE) is a new extraction technique that appeared in the 1970s, and is mainly used for the preparation of analytical samples. SPE has the following obvious advantages: simple, fast, matching SPE unit greatly shortens the analysis time, the processed samples are easy to store and transport, and it is convenient for quality control in different laboratories: not easy to emulsify; suitable for small volume samples Treatment; no need for a large amount of high-purity, flammable, and toxic solvents; there are many types of adsorbents and solvents to choose from, which can provide a variety of experim...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/286
Inventor 王田霖王丹
Owner SHANGHAI UNIV
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