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A technology of functional bacteria and microbial strains, applied in the field of microorganisms, can solve the problems of long recovery period, weak biochemical ability, and slow filtration speed of engineering bacteria, and achieve the effects of shortening the establishment time, strong biochemical ability, and guaranteed quality
Inactive Publication Date: 2010-01-27
HARBIN INST OF TECH
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[0009] The purpose of the present invention is to solve the problem that the current bio-oxidative manganese removal engineering bacteria group is established by natural selection, the biochemical ability is weak, the manganese removal effect is poor, and the filtration speed is slow; Changhe Engineering Bacteria has a long recovery period after being destroyed, and provides a biological manganese removal function bacteria
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specific Embodiment approach 1
[0017] Specific embodiment 1: In this embodiment, the biological manganese removal functional bacterium MB4 (Exiguobacterium sp.) belongs to the genus Exiguobacterium, and was preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee. The preservation date is October 30, 2007, and the preservation number is CGMCC No.2237; Biological Manganese Removal Functional Bacteria MB4 (Exiguobacterium sp.) is a Gram-positive micro-aerobic bacterium, a short bacillus, small in size, 1-1.5 μm in length, 0.5-0.8 μm in width, and blunt at both ends of the cell circle (such as figure 1 and figure 2 shown); brown colonies are formed on the culture medium, opaque, darker in the center, lighter in color around, and the colonies are round; biological manganese removal functional bacteria MB4 (Exiguobacterium sp.) has energy storage particles, no spores and capsules , N, N, N', N'-tetramethyl-p-phenylenediamine (N, N, N', N'-Tetramethyl-p-Pheny...
specific Embodiment approach 2
[0020] Specific embodiment 2: In this embodiment, the biological manganese removal functional bacterium MB4 (Exiguobacterium sp.) is screened from mud taken from the wall of the aeration tank of the biological manganese removal filter of Zhangshi biological manganese removal water plant in Shenyang City, Liaoning Province. 1L medium for screening consists of 0.8g peptone, 0.2g yeast extract, 0.1g MnSO 4 .H 2 O, 0.1 g K 2 HPO 4 , 0.2g MgSO 4 ·7H 2 O, 0.2g NaNO 3 , 0.1g CaCl 2 , 0.1g (NH 4 ) 2 CO 3 , 0.01g FeSO 4 ·7H 2 O and the remainder of distilled water; then sterilized.
[0021] The present embodiment adopts the combination of plate streaking and plate coating method to divide bacteria, and adopts TMPD method (Vandenabeele J, D de Beer et al. Manganese oxidation by microbial consortia from sand filters. Microb. Ecol. 1992, 24: 91-108. ) for the primary screening of the isolated strains. The principle of using the TMPD method in this embodiment is that N, N, N',...
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Abstract
A biological demanganization function strain is disclosed, in particular to a microbe. The invention solves the establishment of the prior biological oxidation demanganization function strain which depends on natural selection, weak biochemical capability and bad demanganization effect and long cultured, tamed, and starting time of the water disposal demanganization biological filter tank establishment and long restoration period after the function strain is broken. The biological demanganization function strain MB4 (Exiguobacterium sp.) belongs to the microminiature Bacterium and is preserved in the Chinese microbe bacterial preservation management committee common microbe center and the preservation date is Oct. 30, 2007 and the preservation number is CGMCC No.2237. The bacterial MB4 is Gram-positive microaerobic bacteria which is bacillus brevis and small in length and is 1-1.5 mum in length, 0.5-0.8 mum in breadth. Two ends of the cell are blunt circle and form brown colony on the culture medium without transparent and the central color is deeper and the peripheral color is lighter. The entire colony is round shape. The strain MB4 has power storage particle without spore and capsule. The dyeing of N, N, N', N'-tetramethyl p-phenylene diamine is positive. The biological demanganization function strain MB4 of the invention is provided with Mn 2+ removing ability and the Mn 2+ clearance is over 91%.
Description
technical field [0001] The present invention relates to a microorganism. Background technique [0002] Manganese is an abundant metal element in the earth's crust. The concentration of manganese ions in ordinary water is 0.1-1 mg / L, while the concentration of soluble manganese ions in anaerobic groundwater can be as high as 7-9 mg / L. Soluble manganese can form insoluble precipitates (such as MnO 2 or MnOOH). [0003] MnO 2 is a highly insoluble substance (δ-MnO 2 The solubility kso is only 10 -56 ), which is dark black and can cause significant coloration in groundwater intended for drinking; manganese must therefore be removed from drinking water. my country stipulates that the maximum concentration of manganese ions in drinking water is 0.1mg / L, while the European Union has determined the maximum concentration of manganese ions in drinking water as 0.05mg / L. [0004] The removal of soluble Mn(II) from groundwater is usually accompanied by oxidation of manganese ions,...
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