Antigen epitope and mutant of lyase in gamma bacteriophage of anthrax bacillus, and application

A technology of phage lyase and Bacillus anthracis, applied to antigenic epitopes and mutants of Bacillus anthracis T phage lyase, application in preventive and/or therapeutic drugs, and diagnostic field

CN101003569AInactive Publication Date: 2007-07-25INST OF BIOENG ACAD OF MILITARY MEDICAL SCI OF THE CHINESE
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
Publication Date
2007-07-25
Estimated Expiration
Not applicable · inactive patent

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Abstract

This invention discloses Bacillus anthracis gamma-phase lyase epitope, its lyase mutant and application. This invention provides the epitope of Bacillus anthracis gamma-phase lyase PlyG, and mutant lyase MPlyG. The epitope is 164th-172nd amino acid residues of lyase PlyG, and is shown in SEQ ID No.4. Mutant lyase MPlyG is derived from lyase PlyG by deleting one or more of 164th-172nd amino acid residues. Animal experiments show that compared with PlyG, MPlyG has lower antigenicity, lower immunogenicity, and similar lyase activity. Therefore, MPlyG can be used to prevent and treat charbon and manufacture corresponding drugs.
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Description

technical field

[0001] The present invention relates to an antigenic epitope of Bacillus anthracis γ phage lyase and its mutant and its application, in particular to an antigenic epitope of Bacillus anthracis T phage lyase and its mutant in the diagnosis, prevention and / or preparation of anthrax application in therapeutic drugs. Background technique

[0002] Anthrax is a zoonotic acute infectious disease caused by Bacillus anthracis. The main susceptible animals are sheep, cattle, horses and other herbivorous livestock. The blood of dead animals that die of anthrax does not coagulate, and often discharges a large amount of blood with natural channels. Germs. Due to the different routes of infection, anthrax is clinically divided into skin type, gastrointestinal type and inhalation infection type. Cutaneous anthrax is more common, mainly manifested as skin anthrax pain in exposed parts; gastrointestinal anthrax is relatively rare, in addition to fever and gastrointestinal s...

Examples

Embodiment 1

[0044] Embodiment 1, the acquisition of Bacillus anthracis gamma phage lyase mutant MPlyG

[0045] 1. Preparation of anti-PlyG antibody

[0046] Get 0.5mg of purified PlyG (for its prokaryotic expression and purification method, see the patent application of application number: 200610057298.5, the title of the invention is "a method for expressing Bacillus anthracis gamma phage lyase and its special gene"), and compare it with complete Freund's The adjuvant was mixed at a volume ratio of 1:1 to immunize rabbits, and immunized once every 10 days. After 35 days, blood was collected from the heart to obtain antiserum, and then the antiserum was used to detect whether anti-PlyG antibody was produced in the serum by Western Blot method. The detection results are shown in FIG. 1 , indicating that anti-PlyG antibodies that can specifically bind to PlyG were produced in the serum.

[0047] 2. Construction of Escherichia coli expression vector containing PlyG deletion mutant gene

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