Method and reagent kit for protein N-terminal peptide specific identification and sequencing

Abstract

The invention relates to a method and kit of particularity recognition and mass spectrum sequencing of protein N-end peptide section. The method and kit can be used for particularity recognition of protein N-end peptide section, increase determinacy and reliability of identification for protein N-end peptide section, and be adapted for protein identification and N-end analysis in biology, medicine and medicament research and application field.

Description

technical field [0001] The invention relates to a new method and kit for specific identification and mass spectrometry sequencing of protein N-terminal peptides. Background technique [0002] As we all know, the N-terminal information of proteins is very important for the structure determination and function elucidation of proteins. On the one hand, the N-terminal sequence tags of proteins are highly specific. Statistically, 43% to 83% of proteins have a unique N-terminal 4-residue tag [Wilkins, M.R., Gasteiger, E., Tonella, L., Ou, K. et al, J.Mol.Biol.1998, 278 , 599-608.]. On the other hand, the N-terminal sequence of the protein helps to confirm the N-terminal processing of the protein [Chung, J.J., Shikano, S., Hanyu, Y., Li, M., Trends Cell Biol.2002, 12, 146-150. ]. Although the N-terminal peptides of proteins contain important biological information, it is difficult to specifically recognize and identify the N-terminal peptides of proteins due to the production o...

AI Technical Summary

Problems solved by technology

Although the N-terminal peptides of proteins contain important biological information, due to the production of a large number of non-terminal peptides during proteolytic digestion, it is difficult to specifically recognize and identify the N-terminal peptides of proteins

Edman degradation is a conventional method for N-terminal identification of proteins, but relatively high sequencing costs and lack of non-N-terminal peptide information have become the shortcomings of this sequencing method

With the rapid development of mass spectrometry technology, although there are some methods in the literature to enrich or identify the N-terminus of proteins [Yamaguchi, M., Nakazawa, T., Kuyama, H., Obama, T. et al, Anal.Chem.2005, 77, 645-651], but currently there is no simple and easy method to specifically identify the N-terminus of proteins from complex protease-cleaved peptides and sequence them effectively

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