Method and reagent kit for protein N-terminal peptide specific identification and sequencing

A protein and kit technology, applied in the field of specific recognition and sequencing of protein N-terminal peptides and kits, can solve the difficulties of specific recognition and identification of N-terminal peptides, high sequencing costs, non-N-terminal Problems such as the lack of peptide information, to achieve the effects of wide practicability, quality improvement, and increased certainty

Inactive Publication Date: 2007-10-17
INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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Problems solved by technology

Although the N-terminal peptides of proteins contain important biological information, due to the production of a large number of non-terminal peptides during proteolytic digestion, it is difficult to specifically recognize and identify the N-terminal peptides of proteins
Edman degradation is a conventional method for N-terminal identification of proteins, but relatively high sequencing costs and lack of non-N-terminal peptide information have become the shortcomings of this sequencing method
With the rapid development of mass spectrometry technology, although there are some methods in the literature to enrich or identify the N-terminus of proteins [Yamaguchi, M., Nakazawa, T., Kuyama, H., Obama, T. et al, Anal.Chem.2005, 77, 645-651], but currently there is no simple and easy method to specifically identify the N-terminus of proteins from complex protease-cleaved peptides and sequence them effectively

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  • Method and reagent kit for protein N-terminal peptide specific identification and sequencing
  • Method and reagent kit for protein N-terminal peptide specific identification and sequencing
  • Method and reagent kit for protein N-terminal peptide specific identification and sequencing

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Embodiment Construction

[0027] The following examples will further explain the present invention, but the present invention is not limited to these examples, and these examples do not limit the scope of the present invention in any way. Certain changes and adjustments made by those skilled in the art within the scope of the claims should also be deemed to belong to the scope of the present invention.

[0028] Specific identification and sequencing of N-terminal peptides in bovine serum albumin

[0029] 1.1 Take 500μg bovine serum albumin, dissolve in 500μl 0.1% SDS, 1M o-methylisourea sodium hydroxide solution (pH12) and react at 37°C for 4 hours, divide the sample into three parts and add 3μl 1M d 0 - Acetic anhydride, mixed 1:1 d 0 / d 6 -Acetic anhydride, d 6 -Acetic anhydride, after 2 hours of reaction at room temperature, 100 mM Tris / HCl was added to terminate the reaction. 10mM final concentration of DTT was reacted at 37°C for 1 hour, 25mM final concentration of iodoacetamide was reacted at...

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Abstract

The invention relates to a method and kit of particularity recognition and mass spectrum sequencing of protein N-end peptide section. The method and kit can be used for particularity recognition of protein N-end peptide section, increase determinacy and reliability of identification for protein N-end peptide section, and be adapted for protein identification and N-end analysis in biology, medicine and medicament research and application field.

Description

technical field [0001] The invention relates to a new method and kit for specific identification and mass spectrometry sequencing of protein N-terminal peptides. Background technique [0002] As we all know, the N-terminal information of proteins is very important for the structure determination and function elucidation of proteins. On the one hand, the N-terminal sequence tags of proteins are highly specific. Statistically, 43% to 83% of proteins have a unique N-terminal 4-residue tag [Wilkins, M.R., Gasteiger, E., Tonella, L., Ou, K. et al, J.Mol.Biol.1998, 278 , 599-608.]. On the other hand, the N-terminal sequence of the protein helps to confirm the N-terminal processing of the protein [Chung, J.J., Shikano, S., Hanyu, Y., Li, M., Trends Cell Biol.2002, 12, 146-150. ]. Although the N-terminal peptides of proteins contain important biological information, it is difficult to specifically recognize and identify the N-terminal peptides of proteins due to the production o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N1/28
Inventor 钱小红刘新周春喜应万涛
Owner INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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