Extract of sparassis crispa MH-3 bacterial strain and method for preparing the same and use thereof

A hydrangea and extract technology, applied in the field of hydrangea MH-3 strain NIBH FERMP-17221 extract, can solve the problems of difficult artificial cultivation and slow growth

Inactive Publication Date: 2008-01-30
中岛 三博
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Hydrangea is an extremely rare type of mushroom. It tastes good, but it grows slowly and is difficult to cultivate artificially.

Method used

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  • Extract of sparassis crispa MH-3 bacterial strain and method for preparing the same and use thereof
  • Extract of sparassis crispa MH-3 bacterial strain and method for preparing the same and use thereof
  • Extract of sparassis crispa MH-3 bacterial strain and method for preparing the same and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The hydrangea MH-3 strain was pulverized into powder, and a weight ratio mixture of chloroform:methanol:water=200:200:60 was prepared, and 25 g of the pulverized dry powder was subjected to degreasing treatment for 2 days at room temperature.

Embodiment 2

[0046] After obtaining the extract (test sample 2) in Example 1, Example 2 was carried out. 500 ml (4°C) of 10% NaOH alkaline water to which 5% urea was added was added to the residue obtained in Example 1, and extraction treatment was performed for 2 days to obtain an extract (test sample 3). After dialysis, ethanol precipitation, dehydration, and drying were performed to obtain 4.93 g of cold alkali extract B.

Embodiment 3

[0048]After obtaining the extract (test sample 3) obtained in Example 2, Example 3 was carried out. 500 ml (65 degrees) of hot alkaline water of 10% NaOH to which 5% urea was added was added to the residue obtained in Example 2, and extraction treatment was performed for 1 hour to obtain an extract (test sample 4). After dialysis, ethanol precipitation, dehydration, and drying were performed to obtain 1 g of hot alkali extract C.

[0049] The solid matter after the degreasing treatment was air-dried, put into an autoclave, added 500 ml of hot water, and subjected to hot water extraction for 2 hours to obtain an extract (test sample 1). The residue after extraction was put into an autoclave, 500 ml of water was added, and hot water extraction was further performed for 2 hours to obtain an extract (test sample 2). Mix the extracts obtained by hot water extraction (test samples 1 and 2), add a large amount of ethanol (200ml), centrifuge at 5000rpm for 5 minutes to precipitate, a...

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Abstract

The present invention discloses an extract of Sparassis Crispa MH-3 strain NIBH FERM P-17221, which takes the Beta-1, 3-glucan as the main ingredient. The present invention also discloses a preparation method of the extract of sparassis crispa MH-3 strain. In addition, the present invention also discloses the application of the extract of sparassis crispa MH-3 strain in the preparation of foods, beverages and health care products and the application of which in the preparation of anti-tumor drugs.

Description

technical field [0001] The invention relates to an extract of Hydrangea MH-3 strain (Sparassis Crispa) NIBH FERMP-17221; in addition, the invention also relates to a preparation method and application of the Hydrangea MH-3 strain extract. Background technique [0002] In the past, many wild or cultivated fungi were used to extract various active ingredients and make them into functional foods, beverages and medicines. Although the technology for extracting biologically active substances from various mushrooms is mature, it is difficult to clarify their respective biological activities due to the large number of strains and extremely complex components. [0003] Over the years, people have conducted a lot of research and exploration on the immune activity and anti-tumor effect of mushroom polysaccharides. So far, many mushroom polysaccharide products have been developed, such as jitake mushroom, maitake mushroom, ganoderma lucidum, etc., and have obtained certain social and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/74A61K31/716A61P35/00A23L1/30A23L2/52A23K1/16A23K20/163A23L33/125A61K36/07
Inventor 中岛三博
Owner 中岛 三博
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