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Reagent case for lung carcinoma susceptibility detection through XRCC1 gene and ERCC2 gene

A kit and susceptibility technology, applied in the field of kits for detecting susceptibility to lung cancer, can solve problems such as inability to correctly identify DNA damage sites, accumulation of carcinogenic damage, and inability to perform base excision repair correctly

Inactive Publication Date: 2008-03-12
XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Loss of function of ERCC2 results in the inability of the essential transcription factor complex to correctly recognize sites of DNA damage, thereby preventing base excision repair from proceeding correctly, leading to the accumulation of oncogenic lesions on DNA

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1. Use of detection kits

[0027] Step 1: Extraction of DNA template

[0028] Genomic DNA of oral epithelial cells was extracted by silica gel adsorption.

[0029] Step 2: Real-time quantitative PCR reaction

[0030] Use a fluorescent quantitative PCR detection kit that can detect susceptibility to lung cancer, which contains the following primer pairs and fluorescent probes:

[0031] Sense primer 1: 5'-TAACTGGCATCTTCACTTCT-3' (SEQ ID NO: 1) with a Tm value of 56°C

[0032] Antisense primer 1: 5'-TCCAGATTCCTGGCATTGC-3' (SEQ ID NO: 2) with a Tm value of 58°C

[0033] Sense primer 2: 5'-ACTCAGGAGTCACCAGGAA-3' (SEQ ID NO: 3) with a Tm value of 58°C

[0034] Antisense primer 2: 5'-TCTGTTCTTCTGCAGGAGGA-3' (SEQ ID NO: 4) with a Tm value of 58°C

[0035] Fluorescent probe 1: 5'-CGGCTGCCCTCCCAGAGG-3' (SEQ ID NO: 5) with a Tm value of 64°C

[0036] Fluorescent probe 2: 5'-AATCTGCTCTTATCCTCTGCAGC-3' (SEQ ID NO: 6) with a Tm value of 66°C

[0037] Sense primer 1, anti...

Embodiment 2

[0042] Example 2. Services that guide people to proactively prevent lung cancer

[0043] At present, Shanghai Zhujian Bioengineering Co., Ltd. has launched this service.

[0044] Step 1: DNA Extraction

[0045] The subjects were consulted before the service, and the subjects signed the informed consent and filled out the questionnaire on living and eating habits. According to the instructions in the sampling box, the oral epithelial cells were sampled with oral swabs, and the DNA of the oral epithelial cells was extracted by silica gel adsorption.

[0046] Step 2: Genotyping Assays

[0047] Using the kit provided by the invention, simultaneously perform fluorescence quantitative PCR detection on the SNP site rs25487 on the XRCC1 gene and the SNP site rs13181 on the ERCC2 gene of the DNA of the subject to be tested, and determine the genotypes of the two SNPs sites.

[0048] Step 3: Instruct people to be proactive in preventing lung cancer

[0049] Through the analysis of t...

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Abstract

The invention discloses a reagent box for detecting lung cancer susceptibility. The reagent box comprises a pair of specificity primers for detecting concurrently no. rs25487 SNP site of XRCC1 gene and no. rs13181 SNP site of ERCC2 gene, a specificity fluorescent probe and an ordinary assembly for fluorescent quantitative PCR detection. The reagent box predicts the susceptibility of individuals on lung cancer by detecting and analyzing currently the type of carried gene at SNPs site of individual XRCC1 and ERCC2 gene.

Description

technical field [0001] The invention relates to a kit for detecting disease susceptibility, in particular to a kit for detecting susceptibility to lung cancer, through simultaneous detection of X-ray repair cross-complementing gene 1 (XRCC1) Single nucleotide polymorphism (SNP) sites and excision repair complex 2 (Excision Repair Cross-complementing Rodent Repair Deficiency, Complementation Group 2, ERCC2) single nucleotide polymorphism sites to predict the susceptibility of individuals to lung cancer emotional. Background technique [0002] Primary bronchial lung cancer, referred to as lung cancer, is one of the most common malignant tumors in the world and a serious threat to human health and life. Tumor cells originate from the bronchial mucosa or glands, and often have regional lymph node and hematogenous metastasis. In the early stage, respiratory symptoms such as irritating cough and blood in the sputum are often present. The speed of disease progression is related to...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 冯哲民邹祖烨
Owner XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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