Method for producing L-threonine

A technology of threonine and culture method, applied in the direction of animal feed, additional food elements, animal feed, etc.

Active Publication Date: 2008-06-11
AJINOMOTO CO INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in terms of the fermentative production of L-threonine, the effects of modulating and reducing the sulfur concentration in the fermentation medium are unknown

Method used

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  • Method for producing L-threonine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0106] Example 1: Effect of Adjustment of Initial Sulfur Addition in Fed-Batch Culture

[0107] First, in fed-batch culture, the effect of limiting sulfur in the main culture initial medium to a predetermined concentration or lower during L-threonine production was examined.

[0108] Cultivate the VKPM B-5318 strain on LB agar medium plate (10g / L tryptone, 5g / L yeast extract, 5g / L NaCl, 15g / L agar) containing 20mg / L streptomycin sulfate at 37°C 24 hours, scrape off 1 / 10 of the cells on the plate from one plate and inoculate into 50 mL LB medium containing 20 mg / L streptomycin sulfate (10 g / L tryptone, 5 g / L yeast extract, 5g / L NaCl), at 40°C and 144rpm for 6 hours seed culture.

[0109] After completion of the seed culture, a seed culture medium in a volume equivalent to 16% of the volume of the main culture medium was inoculated into a 1-L small fermenter containing 300 mL of the main culture medium, and cultured at 40° C. and pH 7.0. The composition of the main culture me...

Embodiment 2

[0125] Example 2: Effect of adjustment of sulfur in the feed medium in fed-batch culture

[0126] First, in the same manner as in Example 1, the VKPM B-5318 strain was placed on the LB agar medium plate containing 20 mg / L streptomycin sulfate (10 g / L tryptone, 5 g / L yeast extract, 5 g / L L NaCl, 15g / L agar) at 37°C for 24 hours, scrape off 1 / 10 of the cells on the plate from a plate, and inoculate 50 mL of LB culture containing 20 mg / L streptomycin sulfate in a baffled flask Seed culture was carried out at 40° C. and 144 rpm for 6 hours in medium (10 g / L tryptone, 5 g / L yeast extract, 5 g / L NaCl).

[0127] After completion of the seed culture, a seed culture medium in a volume equivalent to 16% of the volume of the main culture medium was inoculated into a 1-L small fermenter containing 300 mL of the main culture medium, and cultured at 40° C. and pH 7.0. The main culture medium had the same components as those used in Example 1.

[0128] The culture was adjusted to pH 7.0 du...

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Abstract

The present invention describes a method for producing L-threonine, which comprises the steps of: culturing a microorganism belonging to the genus Escherichia having the ability to produce L-threonine in an environment containing a carbon source, a nitrogen source, and a sulfur source fermentation medium, and collecting L-threonine, wherein the sulfur concentration in the medium is adjusted so as to be at a predetermined level or lower.

Description

technical field [0001] The present invention relates to techniques used in the fermentation industry. More specifically, the present invention relates to a method for efficiently producing L-threonine by fermentation using bacteria of the genus Escherichia. L-threonine is one of essential amino acids, and is useful as an ingredient in nutritional mixtures for medical purposes. It is also used in various ways as an animal feed additive, and as a reactant in the pharmaceutical and chemical industries. Background technique [0002] L-amino acids such as L-threonine and L-isoleucine are industrially produced by fermentation using amino acid-producing bacteria such as coryneform bacteria having the ability to produce these L-amino acids. bacteria) or bacteria of the genus Escherichia. As these amino acid-producing bacteria, bacterial strains isolated from nature or artificial mutants of these bacterial strains are used. Recombinants of bacterial strains in which L-amino acid ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/08A23K1/00
CPCC12P13/08A23K1/1634A23K20/142
Inventor 辻雄一郎加藤直人小山直人城永佑志
Owner AJINOMOTO CO INC
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