52 results about "Genus Escherichia" patented technology

There is disclosed a method for producing L-threonine using bacterium belonging to the genus Escherichia wherein the bacterium has been modified to enhance an activity of aspartate-β-semialdehyde dehydrogenase.

L-Cysteine is produced by culturing a bacterium belonging to the genus Escherichia having an L-cysteine producing ability and modified so that cystathionine-β-lyase activity or cystathionine-β-lyase activity and tryptophanase activity should be reduced or eliminated in a medium to produce and accumulate L-cysteine in the medium and collecting the L-cysteine from the medium.

Amino acids such as threonine, homoserine, isoleucine, lysine, valine and tryptophan are produced using a bacterium belonging to the genus Escherichia which has been constructed from a sucrose non-assimilative strain belonging to the genus Escherichia and which harbors sucrose non-PTS or PTS genes and has an ability to produce the amino acid.

The invention discloses use of Escherichia coli S17-3 for the production of 2'-fucosyllactose. The preservation number of the Escherichia coli S17-3 is CCTCC 2018200. The Escherichia coli S17-3 with high yield of colanicacid is used for denovo synthesizing of 2'-fucosyllactose by using lactose as a sole substrate, and no synthetic route for exogenously expressing GDP-L-fucose exists. According toa fermentation method of recombinant escherichia coli for producing 2'-fucosyllactose, the fermentation output is increased by more than 10 times, the highest output reaches 617.0 mg / L, and the outputis greatly increased.

The invention provides methods for producing purine base analogues, purine nucleosides, and purine nucleotides, such as inosine and 5'-inosinic acid, which include using a bacterium belonging to the genus Bacillus or to the genus Escherichia wherein the purine productivity of said bacterium is enhanced by increasing an activity of the YdhL protein. The invention also provides the amino acid sequence of the YdhL protein from Bacillus amyloliquefaciens and the gene encoding it.

The present invention describes a method for producing L-threonine, which comprises the steps of: culturing a microorganism belonging to the genus Escherichia having the ability to produce L-threonine in an environment containing a carbon source, a nitrogen source, and a sulfur source fermentation medium, and collecting L-threonine, wherein the sulfur concentration in the medium is adjusted so as to be at a predetermined level or lower.

A method for comparing the variable reactivity of multiple, differentially mutated copies of 16S subsequences found in a number of ribosomal RNA operons of a single bacterial cell is described. The application of this method for distinguishing between closely related organisms, such as the genera Escherichia and Shigella, and between species of Shigella including S. boydii, S. dysenteriae, S. flexneri, and S. sonnei using nucleic acid probes is also presented.

The present invention relates to microorganisms of Escherichia coli having enhanced L-tryptophan productivity and to a method for producing L-tryptophan using the same. More particularly, the present invention relates to an Escherichia coli variant in which repression and attenuation control of the tryptophan operon is released and accumulation of anthranilate is reduced and thereby enhancing L-tryptophan productivity. The present invention also relates to a method for producing L-tryptophan using the Escherichia coli variant.

L-arginine, citrulline and pyrimidine derivatives including orotic acid, uridine, uridine 5'-monophosphate (UMP), cytidine and cytidine 5'-monophosphate (CMP) are produced using a bacterium belonging to the genus Escherichia harboring a mutant carbamoylphosphate synthetase in which the amino acid sequence corresponding to positions from 947 to 951 in a wild type carbamoylphosphate synthetase is replaced with any one of amino acid sequences of SEQ ID NOS: 1 to 9, and feedback inhibition by uridine 5'-monophosphate in the bacterium is desensitized.

There is provided a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to the genus Escherichia or Pantoea, having the glycogen biosynthesis pathway disrupted.

The present invention relates to an Escherichia coli mutant strain having improved L-threonine productivity by the introduction of permease derived coryneform bacteria, and a method for producing L-threonine using the same.

The invention discloses healthy human intestinal escherichia coli and application thereof and relates to the field of microbial technology. The escherichia coli JL-3 belongs to Escherichia, and the preservation number is CGMCC No. 14074. The escherichia coli can remarkably prolong average lifetime of caenorhabditis elegans, can remarkably enhance heat-stress resistance of caenorhabditis elegans, can be settled in caenorhabditis elegans, has good gastrointestinal operation endurance ability, can generate nitric oxide signal molecule, and has a wide prospect of being developed into health food or medicines.

There is provided a method for producing L-threonine, L-valine, L-proline, L-leucine, L-methionine and L-arginine using bacterium belonging to the genus Escherichia wherein L-amino acid productivity of the bacterium is enhanced by enhancing an activity of proteins coded by b2682 and b2683 genes, or protein coded by b1242 or b3434 gene.

The invention represents a method for preparing amino acid L-threonine by using bacterium belonging to Escherichia genus. This bacterium shows ability for production of L-threonine and modified by so manner that expression of gene chosen from the group glk, pgi, pfkA, tpiA, gapA, pgk, eno and pykA encoding glycogenolysis enzyme is enhanced.

The present invention provides a method for producing L-amino acids or salts thereof by fermentation using a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to overexpress the yajL gene.

The present invention provides a method for producing an L-amino acid such as a branched-chain L-amino acid by fermentation using a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to attenuate expression of the gshA gene.

There is disclosed a method for producing L-threonine using bacterium belonging to the genus Escherichia wherein the bacterium has L- theonine productivity and has been modified to enhance an activity of aspartate aminotransferase.

The present invention describes novel bacterial L-amino acids α-ligases, which catalyzing reaction of dipeptide formation having an acidic L-amino acid such as L-Asp or L-Glu at the N-terminus. The method for producing dipeptides using said L-amino acids α-ligases and a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to contain the DNA encoding said L-amino acids α-ligases, is described.

A transformed microorganism having an ability to produce a polyhydroxyalkanoate includes a polyhydroxyalkanoate synthase gene and a gene encoding a chaperone belonging to the ClpB family. The gene encoding the chaperone belonging to the ClpB family is a gene which has been introduced into the transformed microorganism or whose expression has been enhanced in the transformed microorganism. The chaperone belonging to the ClpB family may be derived from the genus Cupriavidus, Escherichia, or Saccharomyces. A polyhydroxyalkanoate can be produced by culturing the transformed microorganism.

The invention provides an enterobacter carrying a CD176 antigen and application thereof, the 16S rRNA gene sequence of the enterobacter has 99% consistency with the 16S rRNA gene sequence of Escherichia coli, the enterobacter can express the CD176 antigen and generate a CD176 antibody through immune induction, and the enterobacter is preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.27745. The bacterium can be used as an antigen for inducing CD176-targeted immunoreaction, can be used as an engineering bacterium, and has wide application.

The invention discloses a modified Escherichia coli engineering bacterium and a method for producing citramalic acid by using the modified Escherichia coli engineering bacterium. According to the method, Escherichia coli S17-3 is taken as an original strain, and a T7 RNA polymerase gene for identifying a T7 promoter is inserted into a genome of the original strain, so that the modified escherichia coli engineering strain is obtained. The modified escherichia coli engineering bacteria further highly express citramalic acid synthase genes and one or more functional proteins of an inactivation auxiliary response factor RcsA, a response regulation factor RcsB, transmembrane sensing kinase RcsC, phosphate transporter RcsD and outer membrane lipoprotein RcsF. The invention provides novel industrial escherichia coli and a fermentation culture method, the strain is easy to culture, citramalic acid can be efficiently biosynthesized by using a cheap carbon source, and the production efficiency is higher than that of the existing biosynthesis method.

The present application discloses Zymomonas mobilis endogenous promoter mutants which guide the expression of heterologous nucleic acids that can be chimeric-linked, promote the expression of heterologous nucleic acids, and at the same time, these promoter mutants are high-expression promoters having a significantly enhanced function relative to wild-type promoters, the present invention relates to chimeric genes for expressing chimeric genes in cells of Zymomonas and / or Escherichia.

The invention provides methods for producing purine base analogues, purine nucleosides, and purine nucleotides, such as inosine and 5'-inosinic acid, which include using a bacterium belonging to the genus Bacillus or to the genus Escherichia wherein the purine productivity of said bacterium is enhanced by increasing an activity of the YdhL protein. The invention also provides the amino acid sequence of the YdhL protein from Bacillus amyloliquefaciens and the gene encoding it.

The present invention provides a method for producing L-amino acids by fermentation using a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to attenuate expression of the yjjK gene.

Described herein is an extract of a herbal composition comprising at least two different dry plants, the compounds are useful as antimicrobial and / or antibiotic film agents in the treatment or prevention of microbial infections caused by bacteria (e.g., Escherichia, Klebsiella, Listeria, Pseudomonas, Salmonella, Streptococcus, or Staphylococcus) or by fungi, as well as in the treatment or prevention of microbial infections caused by bacteria (e.g., Escherichia, Klebsiella, Listeria, Pseudomonas, Salmonella, Streptococcus, or Staphylococcus). It has been found that in such an extract, the active ingredients exert their biological effects in a synergistic manner. The extracts may form active ingredients of food supplements, nutraceuticals, pharmaceutical or cosmetic compositions or functional foods or food additives. Also described herein are methods for preparing the extracts.

The present invention relates to an Escherichia coli mutant strain in which L-threonine productivity is improved by introducing a permease derived from Corynebacterium and a method for producing L-threonine using the Escherichia coli mutant strain.

There is disclosed a method for producing L-threonine using bacterium belonging to the genus Escherichia wherein the bacterium has L- theonine productivity and has been modified to enhance an activity of aspartate aminotransferase.

The present invention discloses an application of tea lovage polysaccharide in the preparation of a drug for regulating intestinal flora in inflammatory bowel disease, and belongs to the technical field of polysaccharide extraction process optimization and intestinal flora regulation. Specifically, the present invention provides an economical and efficient extraction process and new use of tea lovage polysaccharide for the preparation of a preparation or composition. By increasing the relative abundance of probiotic flora, reducing the relative abundance of harmful bacteria, regulating the structure of intestinal flora, etc., the symptoms of intestinal flora disorder in inflammatory bowel disease are alleviated and treated. Tea lovage polysaccharide improves ulcerative colitis in mice by upregulating the relative abundance of probiotics such as Bacteroides and Faecalibacterium prausnitzii, and downregulating the relative abundance of pathogenic bacteria such as Proteobacteria, Gammaproteobacteria, Enterobacteriaceae and Escherichia, thereby reversing the abnormal proliferation of bacteria in mice with inflammatory bowel disease and improving the disordered intestinal flora in mice with inflammatory bowel disease.

A bacterium belonging to the genus Escherichia, which is transformed by introducing, into its cells, a DNA coding for a dihydrodipicolinate synthase originating from a bacterium belonging to the genus Escherichia having mutation to desensitize feedback inhibition by L-lysine and a DNA coding for an aspartokinase III originating from a bacterium belonging to the genus Escherichia having mutation to desensitize feedback inhibition by L-lysine; preferably a bacterium belonging to the genus Escherichia in which a dihydrodipicolinate reductase gene and a diaminopimelate dehydrogenase gene originating from Brevibacterium lactofermentum (or a succinyldiaminopimelate transaminase gene and a succinyldiaminopimelate deacylase gene) are further enhanced, is cultivated in an appropriate medium, L-lysine is produced and accumulated in a culture thereof, and L-lysine is collected from the culture.