39 results about "Genus Escherichia" patented technology

There is disclosed a method for producing L-threonine using bacterium belonging to the genus Escherichia wherein the bacterium has been modified to enhance an activity of aspartate-β-semialdehyde dehydrogenase.

Amino acids such as threonine, homoserine, isoleucine, lysine, valine and tryptophan are produced using a bacterium belonging to the genus Escherichia which has been constructed from a sucrose non-assimilative strain belonging to the genus Escherichia and which harbors sucrose non-PTS or PTS genes and has an ability to produce the amino acid.

The present invention relates to microorganisms of Escherichia coli having enhanced L-tryptophan productivity and to a method for producing L-tryptophan using the same. More particularly, the present invention relates to an Escherichia coli variant in which repression and attenuation control of the tryptophan operon is released and accumulation of anthranilate is reduced and thereby enhancing L-tryptophan productivity. The present invention also relates to a method for producing L-tryptophan using the Escherichia coli variant.

The present invention relates to an Escherichia coli mutant strain having improved L-threonine productivity by the introduction of permease derived coryneform bacteria, and a method for producing L-threonine using the same.

The invention discloses healthy human intestinal escherichia coli and application thereof and relates to the field of microbial technology. The escherichia coli JL-3 belongs to Escherichia, and the preservation number is CGMCC No. 14074. The escherichia coli can remarkably prolong average lifetime of caenorhabditis elegans, can remarkably enhance heat-stress resistance of caenorhabditis elegans, can be settled in caenorhabditis elegans, has good gastrointestinal operation endurance ability, can generate nitric oxide signal molecule, and has a wide prospect of being developed into health food or medicines.

There is provided a method for producing L-threonine, L-valine, L-proline, L-leucine, L-methionine and L-arginine using bacterium belonging to the genus Escherichia wherein L-amino acid productivity of the bacterium is enhanced by enhancing an activity of proteins coded by b2682 and b2683 genes, or protein coded by b1242 or b3434 gene.

The present invention provides a method for producing an L-amino acid such as a branched-chain L-amino acid by fermentation using a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to attenuate expression of the gshA gene.

The present invention describes novel bacterial L-amino acids α-ligases, which catalyzing reaction of dipeptide formation having an acidic L-amino acid such as L-Asp or L-Glu at the N-terminus. The method for producing dipeptides using said L-amino acids α-ligases and a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to contain the DNA encoding said L-amino acids α-ligases, is described.

The invention provides methods for producing purine base analogues, purine nucleosides, and purine nucleotides, such as inosine and 5'-inosinic acid, which include using a bacterium belonging to the genus Bacillus or to the genus Escherichia wherein the purine productivity of said bacterium is enhanced by increasing an activity of the YdhL protein. The invention also provides the amino acid sequence of the YdhL protein from Bacillus amyloliquefaciens and the gene encoding it.

There is disclosed a method for producing L-threonine using bacterium belonging to the genus Escherichia wherein the bacterium has L- theonine productivity and has been modified to enhance an activity of aspartate aminotransferase.

The invention discloses an ammonium ion resistant colibacillus for producing succinic acid and application thereof and belongs to genus Escherichia. The colibacillus is named as Escherichia coli BER528, and the accession number CCTCC NO is of M2015160. On the anaerobic condition, the strain is capable of resisting high-density ammonium ions and accumulating succinic acid. During anaerobic shake flask fermentation with 0.53mol / LNH4 + added externally, 72h strain DCW is of 1.82g / L, the succinic acid yield is of 11.72g / L, but an original parent strain grows extremely slow, the 72h strain DCW is of 1.14g / L only, and the succinic acid yield is of 2.56g / L. Compared with that of the parent strain, the succinic acid yield is increased by 4.57 times. In the process of anaerobic fermentation with pH regulation through ammonia water by the aid of a 5L fermentation tank for 90h, the mutant strain DCW and final succinic acid yield are of 1.22g / L and 27.32g / L and is of 1.16 times and 1.88 times of those of the parent strain, and the mutant strain BER528 has important social benefit and economic value accordingly.

The invention discloses a method for producing colaric acid by acid-resistant high-density Escherichia coli. The E. coli species Escherichia coli S17-3 can be used to produce colaric acid, and the specific method is: adopt Escherichia coli species Escherichia coli S17-3 as the production strain, cultivate, and collect secretions, separate and obtain Cola acid. The preservation number of the Escherichia coli S17-3 species is: CCTCC 2018200. The present invention produces for the first time a mutant strain of Escherichia coli through acid-induced fermentation at 37°C, not only has high yield, but also has fast cell growth and short fermentation time, and provides a technical method for high-efficiency production of kolamic acid.

The present invention relates to a microorganism of the genus Escherichia in which L-tryptophan productivity is improved by inactivating phosphatase activity. Further, the present invention relates to a method for producing L-tryptophan using the microorganism of the genus Escherichia.

The invention discloses an acid-resistant high-density Escherichia coli and its application, the Escherichia coli species Escherichia coli The deposit number of S17-3 is: CCTCC 2018200. Escherichia coli species mentioned above Escherichia coli In the culture medium, S17‑3 can naturally extend the logarithmic growth phase of Escherichia coli without additional fermentation control, and can make the cell density OD during the overnight culture period 600 The value reaches more than 30, which is more than three times that of other Escherichia coli, and provides an important host cell for the economical and efficient high-density fermentation production of Escherichia coli.

The present invention relates to Corynebacterium sp. that is transformed with an Escherichia sp.-derived fructokinase gene to express fructokinase showing a sufficient activity of converting fructose into fructose-6-phosphate, thereby preventing unnecessary energy consumption, and a method for producing L-amino acids using the strain. The transformed Corynebacterium sp. of the present invention is able to express fructokinase from the Escherichia-derived fructokinase gene to prevent unnecessary energy consumption during fructose metabolism, leading to more cost-effective production of L-amino acids. Therefore, it can be widely used for the effective production of L-amino acids.

The present invention provides a method for producing L-amino acids, such as branched-chain L-amino acids, by fermentation using an Enterobacteriaceae bacterium modified in such a manner as to attenuate the expression of the gshA gene, particularly a bacterium belonging to the genus Escherichia.