Healthy human intestinal escherichia coli and application thereof

A technology of Escherichia coli and healthy people, applied in the field of microorganisms, to achieve the effects of reducing morbidity, prolonging average life expectancy, and improving heat stress resistance

Active Publication Date: 2018-02-02
HEBEI AGRICULTURAL UNIV.
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the isolation of facultative anaerobic or aerobic bacteria from human feces as probiotics

Method used

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  • Healthy human intestinal escherichia coli and application thereof
  • Healthy human intestinal escherichia coli and application thereof
  • Healthy human intestinal escherichia coli and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1. Use sterilized tweezers to take fresh feces samples from healthy people, put them into the LB liquid medium prepared in advance, and cultivate them in an ordinary biochemical incubator for 24 hours, and serially dilute the flora (10 -1 ~10 -8 ), and smeared on LB agar plates, and continued to culture for 1 to 2 days respectively. Colonies with different shapes were picked for pure culture, that is, the picked colonies were streaked on the plate, and the experiment was repeated at least three times. The resulting pure cultures were frozen at −80 °C in 50% glycerol. Morphological observation, physiological and biochemical analysis and 16S rDNA sequence analysis were carried out on the isolated single colonies.

[0035] 2. Screening of human intestinal facultative anaerobic bacteria with life-prolonging effect on Caenorhabditis elegans

[0036] (1) Activation of strains: Thaw the pure culture of bacteria preserved in low temperature and inoculate them into LB liquid ...

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PUM

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Abstract

The invention discloses healthy human intestinal escherichia coli and application thereof and relates to the field of microbial technology. The escherichia coli JL-3 belongs to Escherichia, and the preservation number is CGMCC No. 14074. The escherichia coli can remarkably prolong average lifetime of caenorhabditis elegans, can remarkably enhance heat-stress resistance of caenorhabditis elegans, can be settled in caenorhabditis elegans, has good gastrointestinal operation endurance ability, can generate nitric oxide signal molecule, and has a wide prospect of being developed into health food or medicines.

Description

technical field [0001] The invention relates to the technical field of microorganisms. Background technique [0002] Human aging is an inevitable physiological process, and it is a major problem that human beings have been studying but have not been able to solve. At present, our country has entered an aging society, and the ensuing senile diseases have increased significantly, which has brought great pressure and challenges to our country's medical and health care work. Therefore, it is a major problem to be solved urgently to effectively reduce the senile diseases caused by the aging of the body through suitable anti-aging methods. Caenorhabditis elegans is a multicellular eukaryote that feeds on bacteria because of its small body and short lifespan. Due to its own advantages in genetics and developmental biology, Caenorhabditis elegans has become a research tool for studying the regulation mechanism of lifespan and aging in animals. Important animal model (Brenner 1974,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/74A61P39/00A61P39/06A61P37/04A61P9/00A61P25/00A61P15/00A23L33/135C12R1/19
CPCA23V2002/00A61K2035/11C12N1/205C12R2001/19A23V2200/322A23V2200/324A23V2200/326Y02A50/30
Inventor 王秀伶李晓蕾杨露
Owner HEBEI AGRICULTURAL UNIV.
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