Method for constructing external model dynamically simulating human liver cancer metastasis

A liver cancer metastasis and dynamic simulation technology, applied in biochemical equipment and methods, animal cells, vertebrate cells, etc., can solve the problems that the liver cancer metastasis model does not take into account

Inactive Publication Date: 2008-06-25
SHANDONG UNIV
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Problems solved by technology

However, this factor has not been taken into account in the current liver cancer metastasis model.
After searching, no papers or patents have been found on the method of constructing an in vitro model for dynamically simulating human liv...

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  • Method for constructing external model dynamically simulating human liver cancer metastasis
  • Method for constructing external model dynamically simulating human liver cancer metastasis
  • Method for constructing external model dynamically simulating human liver cancer metastasis

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Embodiment 1

[0042] 1. Model establishment of anti-cell attachment

[0043] Adherent growth is a prerequisite for the survival of solid tissue cells. Poly-HEMA (purchased from Sigma Company), as an anionic polymer, can be spread on the bottom of the container to prevent the cells from adhering to the wall, so as to construct a cell model in which cells grow in suspension after shedding.

[0044] Dissolve poly-HEMA with 95% ethanol to prepare a solution with a final poly-HEMA concentration of 120mg / ml (i.e. dissolve 2.4g poly-HEMA in 20ml 95% ethanol), shake and mix at 65°C for 8 hours to obtain poly-HEMA HEMA stock solution; then dilute the poly-HEMA stock solution with 95% ethanol at a volume ratio of 1:10 to make a poly-HEMA working solution with a final concentration of 12 mg / ml, store it at 4°C, and set aside; take poly-HEMA Spread the HEMA working solution evenly on the bottom surface of the 6-well cell culture plate, and the final density of the poly-HEMA working solution is 3mg / cm2...

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Abstract

The invention discloses a method for constructing an in vitro model with dynamic simulation of hepatoma metastasis of human beings, comprising construction of a cell attachment resistant model, construction of the hepatoma cell in vitro metastasis model, identification of the constructed hepatoma in vitro metastasis model, etc. The method of the invention applies high metastasis tendency BEL7402 hepatoma cells to simulate and construct the in vitro model for hepatoma cell metastasis by constructing a model which makes the BEL7402 hepatoma cells deviated from attachment of extracellular matrixes and simulating a condition that transitional cells grows by means of anchorage dependence again when meeting adequate environment. By utilization of the model, control study of carcinoma in situ and tumor during the metastatic process can be performed, and the model can be further taken as a monitoring system for detecting biological behaviors of tumor cells during the metastatic process and judging tumor prognosis. The model has important value in researching a clinical tumor metastatic mechanism and controlling screening of metastasis drugs and also can be taken as a platform for preparing tumor metastasis resistant gene vaccine and establishing a tumor metastasis resistant drug screening technology.

Description

technical field [0001] The invention relates to a method for constructing a liver cancer metastasis model system in vitro, in particular to a method for constructing an in vitro model for dynamically simulating human liver cancer metastasis. Background technique [0002] As a malignant tumor with extremely high morbidity and mortality in China, liver cancer has become a long-term problem for clinicians and scientific researchers because of its rapid disease progression, high degree of malignancy, high metastasis rate, and high recurrence rate. Although a small number of patients have achieved long-term survival due to early diagnosis and treatment, the metastasis and recurrence of liver cancer have always been a major obstacle affecting the treatment effect of liver cancer. Clarifying the mechanism of liver cancer metastasis and its early diagnosis and prevention will be the key to further improve the treatment effect of liver cancer. [0003] Liver cancer is a chronic dise...

Claims

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Application Information

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IPC IPC(8): C12N5/08C12N5/09
Inventor 韩丽辉孙汶生张之勇曹莉莉梁晓红曲忠花杜娟马恬侯楠郭春刘玉刚米曰堂李蕊徐俊龙
Owner SHANDONG UNIV
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