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Eupatorium adenophorum extract used as root-growing agent

A technique of extracting Eupatorium adenophorum, applied in the field of pesticides, to achieve the effect of strong rooting

Inactive Publication Date: 2008-08-06
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the use of Eupatorium adenophorum extract as a rooting agent at home and abroad

Method used

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  • Eupatorium adenophorum extract used as root-growing agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] The leaves of Eupatorium adenophorum were collected, dried in the shade, dried in a drying oven at 10°C, and pulverized by a pulverizer. The obtained Eupatorium adenophorum powder was extracted with 1 volume of methanol at 10° C. by leaching for 30 days. Concentrate the filtered extract at 60°C to obtain Eupatorium adenophorum extract.

[0013] The obtained extract was dissolved in distilled water, prepared into a 0.1 mg / mL medicinal solution, and the rooting-promoting activity was measured by the mung bean rooting method.

[0014] Soak the selected mung bean seeds of consistent size and plumpness in hot water at 80°C, and continue soaking for 2 hours after the water cools to room temperature, so that the seeds can fully absorb and swell. The seeds were then placed in a porcelain dish covered with wet gauze and germinated in the dark at 25°C. After 24 hours, select mung bean seedlings that have germinated neatly, sow them in moist fine sand, and culture them under 700...

Embodiment 2

[0021] The seeds of Eupatorium adenophorum were collected, dried in the shade, dried in a drying oven at 50°C, and pulverized by a pulverizer. The obtained Eupatorium adenophorum powder was extracted with 5 times the volume of petroleum ether at 70°C by Soxhlet extraction for 5 days. Concentrate the filtered extract at 50°C to obtain Eupatorium adenophorum extract.

[0022] Dissolve the obtained extract in water, prepare a 0.5 mg / mL medicinal solution, apply it to the seedbed of poinsettia cuttings, apply once every 10 days, and apply three times in total. Water was used instead of medicinal solution as a control, and 0.05 mg / mL ABT rooting powder was used as a control agent. For each treatment of 30 cuttings, 3 repetitions were set. After 30 days, the survival of the cuttings was measured.

[0023] The results showed that 0.5mg / mL Eupatorium adenophorum extract had a significant rooting effect on poinsettia cuttings, the rooting amount of cuttings treated with liquid medic...

Embodiment 3

[0027] The stems of Eupatorium adenophorum were collected, dried in the shade, dried in a drying oven at 100°C, and pulverized by a pulverizer. The obtained Eupatorium adenophorum powder was extracted with 3 times the volume of ethanol at 25° C. by ultrasonic extraction for 1 minute. Concentrate the filtered extract at 60°C to obtain Eupatorium adenophorum extract.

[0028] Dissolve the obtained extract in water, prepare a 1 mg / mL medicinal solution, insert the base of grape branches into the medicinal solution and soak for 30 minutes before cutting, replace the medicinal solution with water as a control, and use 0.05 mg / mL ABT rooting powder as a control agent. For each treatment of 10 grape branches, 3 repetitions were set. Investigate the rooting situation after 30 days, and calculate the rooting promotion rate.

[0029] The results showed that 1mg / mL Eupatorium adenophorum extract had a strong promoting effect on the rooting of grape cuttings, and the rooting promotion r...

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Abstract

The invention relates to Eupatorium adenophorum spreng extract as rooting agent. Root, stem, leaf or seed of the Eupatorium adenophorum spreng are taken as raw materials and are dried, smashed and extracted to obtain the extract as the rooting agent. The invention for the first time adopts the Eupatorium adenophorum spreng extract as the rooting agent and has good effect on promoting of rooting. As the extract adopts the Eupatorium adenophorum spreng as the raw material, the extract can be degraded successfully in nature without residue and environment pollution; the extract is safe for the non-target organism and is environment friendly; meanwhile, the rooting agent provided by the invention which adopts the Eupatorium adenophorum spreng, a worldwide ill weed as the raw material provides a new way to prevent and remove and apply the ill weed.

Description

technical field [0001] The invention belongs to the field of pesticides, and in particular relates to an extract of Eupatorium adenophorum used as a rooting agent. Background technique [0002] Rooting agents are widely used in food crops, economic crops, fruit trees, vegetables, ornamental plants and forest trees, and plant tissue culture. The traditional rooting agents are mainly indole acetic acid, indole butyric acid, naphthalene acetic acid, phenoxy compounds, etc. Indoleacetic acid (IAA) can significantly promote rooting of plant cuttings, but its chemical properties are unstable, decomposed by light, and easily decomposed by indoleacetic acid oxidase in plants. In addition, it is expensive and difficult to popularize and use. Indole butyric acid (IBA) is chemically more stable than IAA, but it is insoluble in water and inconvenient to use. Compared with indolebutyric acid, naphthaleneacetic acid (NAA) is slightly toxic, and it is easy to damage plants if the concent...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/00A01P21/00
Inventor 查友贵王静叶敏肖春
Owner YUNNAN AGRICULTURAL UNIVERSITY
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