Eupatorium adenophorum extract used as root-growing agent
A technique of extracting Eupatorium adenophorum, applied in the field of pesticides, to achieve the effect of strong rooting
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Embodiment 1
[0012] The leaves of Eupatorium adenophorum were collected, dried in the shade, dried in a drying oven at 10°C, and pulverized by a pulverizer. The obtained Eupatorium adenophorum powder was extracted with 1 volume of methanol at 10° C. by leaching for 30 days. Concentrate the filtered extract at 60°C to obtain Eupatorium adenophorum extract.
[0013] The obtained extract was dissolved in distilled water, prepared into a 0.1 mg / mL medicinal solution, and the rooting-promoting activity was measured by the mung bean rooting method.
[0014] Soak the selected mung bean seeds of consistent size and plumpness in hot water at 80°C, and continue soaking for 2 hours after the water cools to room temperature, so that the seeds can fully absorb and swell. The seeds were then placed in a porcelain dish covered with wet gauze and germinated in the dark at 25°C. After 24 hours, select mung bean seedlings that have germinated neatly, sow them in moist fine sand, and culture them under 700...
Embodiment 2
[0021] The seeds of Eupatorium adenophorum were collected, dried in the shade, dried in a drying oven at 50°C, and pulverized by a pulverizer. The obtained Eupatorium adenophorum powder was extracted with 5 times the volume of petroleum ether at 70°C by Soxhlet extraction for 5 days. Concentrate the filtered extract at 50°C to obtain Eupatorium adenophorum extract.
[0022] Dissolve the obtained extract in water, prepare a 0.5 mg / mL medicinal solution, apply it to the seedbed of poinsettia cuttings, apply once every 10 days, and apply three times in total. Water was used instead of medicinal solution as a control, and 0.05 mg / mL ABT rooting powder was used as a control agent. For each treatment of 30 cuttings, 3 repetitions were set. After 30 days, the survival of the cuttings was measured.
[0023] The results showed that 0.5mg / mL Eupatorium adenophorum extract had a significant rooting effect on poinsettia cuttings, the rooting amount of cuttings treated with liquid medic...
Embodiment 3
[0027] The stems of Eupatorium adenophorum were collected, dried in the shade, dried in a drying oven at 100°C, and pulverized by a pulverizer. The obtained Eupatorium adenophorum powder was extracted with 3 times the volume of ethanol at 25° C. by ultrasonic extraction for 1 minute. Concentrate the filtered extract at 60°C to obtain Eupatorium adenophorum extract.
[0028] Dissolve the obtained extract in water, prepare a 1 mg / mL medicinal solution, insert the base of grape branches into the medicinal solution and soak for 30 minutes before cutting, replace the medicinal solution with water as a control, and use 0.05 mg / mL ABT rooting powder as a control agent. For each treatment of 10 grape branches, 3 repetitions were set. Investigate the rooting situation after 30 days, and calculate the rooting promotion rate.
[0029] The results showed that 1mg / mL Eupatorium adenophorum extract had a strong promoting effect on the rooting of grape cuttings, and the rooting promotion r...
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