Clone of gene RID1 for controlling rice floral conversion and heading stage and application thereof

Abstract

The invention relates to the technical field of plant gene engineering, which particularly relates to an isolation and cloning, functional verification and application of gene RIDI which regulates and controls plant(rice) flower formation conversion or / and ear sprouting period. RID1 gene which is cloned by the invention is tested to have the function of regulating and controlling plant (rice) flower formation conversion or / and ear sprouting period through a gene function-complement experiment. Gens and encoding protein which are cloned by the invention can be widely applied in regulating and controlling flowering characteristics of plants and improving child-bearing age of plant varieties and area adaptation range, which has an important application value.

Description

Cloning and application of a gene RID1 controlling flowering transition and heading date in rice technical field The invention relates to the technical field of plant genetic engineering. Specifically, it relates to the cloning, functional verification and application of a gene RID1 for controlling plant (rice) flowering transition or / and heading date. Transforming the gene into the rice plant can start the flowering conversion process of the rice plant and regulate the heading date of the rice. Background technique Flowering (shown as heading in rice) is an important growth and development process for plants to transition from vegetative growth to reproductive growth. The timing of plant flowering is important, especially when the desired product is flowers or seeds derived therefrom, and in order for plants to reproduce sexually successfully, they must flower under suitable environmental conditions. For example, common wild rice is mainly distributed in tropical region...

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Problems solved by technology

For example, Ehd1 and OsMADS51 in rice play a role in promoting flowering under short-day conditions, but their homologous genes do not exist in Arabidopsis; and the key regulatory gene FLC in the autonomous flowering pathway of Arabidopsis is not found in rice. 同源基因(Doi等,Ehd1,aBtyperesponseregulatorinrice,confersshort-daypromotionoffloweringandcontrolsFT-likegeneexpressionindependentlyofHd1,GenesDev(2004):926-936;Kim等,OsMADS51isashort-dayfloweringpromoterthatfunctionsupstreamofEhd1,OsMADS14,andHd3a,PlantPhysiol(2007):145:1484-94;Michaels等 , FLOWERINGLOCUSCencodesnovelMADSdomainproteinthatactsasarepressoroffflowering, PlantCell (1999): 949-56)

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