Method for synthesizing polymyxin E by fermentation method

A technology of polymyxin and fermentation method, applied in the field of fermentation biosynthesis of polymyxin E, can solve the problem of low fermentation unit and achieve the effect of increasing fermentation unit

Inactive Publication Date: 2008-08-06
SHANGHAI INST OF PHARMA IND
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0003] At present, the production of polymyxin E mainly adopts microbial ferm

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Prepare the used substratum of the inventive method according to following formula, wherein substratum a is the formula of the present invention, and substratum b is the formula of comparative literature:

[0017] Medium a: 60g soybean protein isolate, 30g starch, 1g ammonium sulfate, CaCO 3 8g, MgSO 4 ·7H 2 O 0.02g, MnSO 4 ·H 2 O 0.01g, distilled water to 1L.

[0018] Medium b: 0.03L corn steep liquor, 10.0g starch, 8.0g ammonium sulfate, CaCO 3 10.0g, distilled water to 1L.

[0019] Wherein medium a and b are all inserted into the seed culture of AS1.541, the inoculum size is 7%,

[0020] Before disinfection, the pH value was adjusted to 7.2; the temperature was 30°C; the filling volume was 30ml / 250ml; after 40 hours of cultivation, the bottle was placed, and the former obtained 22143.7U / ml polymyxin E, while the latter obtained 16295U / ml polymyxin E.

Embodiment 2

[0022] 60 g of soybean protein isolate in medium a was replaced with 40 g of soybean protein isolate, resulting in 16452.1 U / ml polymyxin E.

Embodiment 3

[0024] 60 g of soybean protein isolate in medium a was replaced with 100 g of soybean protein isolate, resulting in 19072.3 U / ml polymyxin E.

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Abstract

The invention discloses a method for synthesizing polymyxin E through a fermentation method, which comprises: inoculating bacillus polymyxa AS1.541 into culture medium to culture and is characterized in that the concentration of the organic nitrogen source which is used in the culture medium is 4%-10%. The highest fermentation unit of the polymyxin E which is synthesized through fermenting by the method of the invention can reach 22143.7U/ml.

Description

technical field [0001] The invention relates to a method for biosynthesizing polymyxin E by a fermentation method. Background technique [0002] Polymyxin is a general term for a family of basic cyclic polypeptide antibiotics composed of various amino acids and fatty acids produced by Bacillus polymyxa. So far, it has been found that among multiple components of polymyxin A, B, C, D, E, M, etc., only polymyxin B (polymyxinB, polymyxin for short), polymyxin E (polymyxinE, Colistin for short) ) has low toxicity and good antibacterial activity. Therefore, there are relatively many studies on polymyxin B and polymyxin E, and the corresponding products have been used clinically. Their sulfate or methanesulfonate are commonly used clinically, and the latter is mostly used for parenteral administration, and polymyxin is hydrolyzed in tissues to play a role. It can be used to treat some meningitis and dysentery caused by Gram-negative bacteria, especially it can inhibit Pseudomon...

Claims

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Application Information

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IPC IPC(8): C12P21/02C12N1/20C12R1/12
Inventor 齐艳艳陈雪田永辉赵文杰冯军程睛华林纲薛春佳朱裕辉魏晓东
Owner SHANGHAI INST OF PHARMA IND
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