Breeding method of fresh water genuine pearl with pearl

A cultivation method, freshwater technology, applied in biochemical equipment and methods, tissue culture, climate change adaptation, etc.

Inactive Publication Date: 2008-08-27
ZHEJIANG RUANS PEARL LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is simple, can complete the process of inserting nuclei at one time, has a high survival rate, and is suitable for large-scale production. However, due to one-time molding, although the diameter of the beads is larger than that of non-nuclear pearls, it is necessary to reach the jewelry-grade large pearls. certain degree of difficulty

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: The cultivation method of large-nucleus freshwater beads, including nuclear selection-nucleation-cultivation, and nuclear insertion is carried out twice: for the first time, first cultivate small pieces of cell tissue or biological cells with proliferation ability, and adhere them to flat button bead nucleus, and then put the bead nucleus into the mantle of the river mussel or the nucleus in the viscera sac, culture for 15 months, and carry out live clam bead extraction; the second time, insert a 15mm round bead nucleus, insert In the pearl sac formed after the first cultivation of pearls, it is maintained for 40 months. The bead core used for insertion is first soaked in clean water for 30 hours, and then soaked in hot water at 75°C for 35 minutes.

[0027] When cultivating small pieces of cell tissue or biological cells with proliferative ability, a balanced salt solution and medium should be prepared, and the formula is:

[0028] (1) Balanced salt solu...

Embodiment 2

[0042] Embodiment 2: With reference to Example 1, the cultivation method of large-nucleus freshwater pearls includes nuclear selection-nucleation-cultivation, and nuclear insertion is carried out twice: for the first time, first cultivate cell tissue fragments or biological cells with proliferation ability, and then It adheres to the flat button bead nucleus, and then sends the bead nucleus to the mantle of the river mussel or the nucleus in the visceral sac, and cultures it for 20 months to extract the bead from the live clam; the second time, the 15mm round The bead nucleus is inserted into the pearl sac formed after the first cultivation of pearls, and is maintained for 46 months. The bead core used for insertion is first soaked in clean water for 35 hours, and then soaked in hot water at 75°C for 40 minutes.

[0043] When cultivating small pieces of cell tissue or biological cells with proliferative ability, a balanced salt solution and medium should be prepared, and the f...

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Abstract

The invention relates to a method for culturing fresh water large-nucleus pearls, which includes pearl nucleus picking, pearl nucleus inserting and pearl culturing, wherein the pearl nucleus inserting is divided into two steps, including culturing cell tissue mantle or biological cell that has multiplication capacity, adhering to the pearl nucleus, then putting the pearl nucleus into the nucleus site in the mantle and the visceral sac of an anodonta and harvesting pearls from the anodonta after 10 to 20 months' culturing for the first step; inserting the pearl nucleus with a diameter of above 15 mm into the pearl sac and conserving pearl for 30 to 50 months; the culturing of the cell tissue mantle or the biological cell thereof requires the preparation the balanced salt solution and the culture medium. The method is characterized in easy operation, high survival percent, large pearl diameter and thick pearl layer. Pearls of gem grade with the diameter of above 20 mm can be cultured.

Description

technical field [0001] The invention relates to a method for cultivating pearls, in particular to a method for cultivating freshwater large-core pearls. Background technique [0002] Now, in the general method of cultivating pearls in freshwater mussels, small pieces of cells made of the mantle are inserted into the mantle of the mussel, and the late pearls cultivated by this method are called non-nuclear pearls, and their diameter is small. The market price is low, and the economic benefit is not high. The production of nucleated pearls in China is mainly based on seawater nucleated pearls. The reason is that the seawater mother-of-pearl is relatively large, and the core insertion site is not located in the mantle but mainly in the visceral sac. It is difficult to insert cores in freshwater mussels, whether it is in the mantle or the visceral sac. It has not been practically applied in large-scale production, because there are problems such as high mortality rate, high nu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00C12N5/06C12N5/07
CPCY02A40/81
Inventor 阮铁军
Owner ZHEJIANG RUANS PEARL LTD
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