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Stem cell preparation for preventing acute graft versus host disease

A technology of graft-versus-host and stem cell preparations, applied in the field of stem cells and immunology, can solve problems affecting the treatment effect of allogeneic hematopoietic stem cell transplantation, difficult comprehensive treatment schemes, unfavorable immune reconstitution, etc., to promote hematopoietic reconstitution and immune reconstitution, The effect of reducing the severity and reducing the incidence rate

Inactive Publication Date: 2008-09-24
SUN YAT SEN UNIV
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AI Technical Summary

Problems solved by technology

In order to reduce GVHD, hematopoietic cell transplantation with depletion of T cells is usually used, but it is not conducive to immune reconstitution and more prone to post-transplant infection
Because the above problems are intertwined, it is still difficult to find a comprehensive treatment plan that can take into account all aspects, which seriously affects the therapeutic effect of allogeneic hematopoietic stem cell transplantation

Method used

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  • Stem cell preparation for preventing acute graft versus host disease
  • Stem cell preparation for preventing acute graft versus host disease
  • Stem cell preparation for preventing acute graft versus host disease

Examples

Experimental program
Comparison scheme
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Embodiment Construction

[0023] (1) Isolation, identification and passage expansion of mesenchymal stem cells

[0024] Before transplantation, allogeneic healthy bone marrow was collected, and mononuclear cells were harvested for culture. With the help of MSC's adherent characteristics, the medium was changed repeatedly and the suspended cells were discarded, so as to gradually obtain pure bone marrow mesenchymal stem cells. Through morphological observation, flow cytometry analysis, and multi-directional differentiation potential identification of osteogenic, adipogenic, and neural-like cell differentiation, it was confirmed that the isolated cells were mesenchymal stem cells, which were continuously passaged and expanded for future use.

[0025] (2) Preparation of splenocyte suspension

[0026] Take the spleen of the donor rat, cut it into small pieces with scissors, grind it gently with a syringe needle core on a 200-mesh steel mesh, and let it pass through to form a spleen cell suspension. Remove...

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Abstract

The invention belongs to the stem cells and immunology field, and relates to a stem cell preparation for preventing acute graft-versus-host disease. The stem cell preparation for preventing acute graft-versus-host disease of the invention is a stem cell preparation jointly grafted from mesenchymal stem cells and hematopoietic stem cells. The stem cell preparation is infused into the receptors in the human body through vein, the infusion dosage of the mesenchymal stem cells is adjusted according to the receptors, and the infusion dosage of the hematopoietic stem cells refers to the conventional bone marrow transplantation program. The stem cell preparation of the invention is applicable to the patient the bone marrow of which is transplanted, can reduce the outbreak rate of the acute graft-versus-host disease (GVHD), can lower the severity degree obviously, and can provide a new way for comprehensively solving the problems raised after hematopoietic stem cells transplantation.

Description

technical field [0001] The invention belongs to the field of stem cells and immunology, and relates to a new stem cell preparation, in particular to a stem cell preparation for preventing acute graft-versus-host disease. Background technique [0002] Bone marrow transplantation is currently one of the effective methods for the treatment of many malignant blood diseases and autoimmune diseases. Due to the increasing popularity of allogeneic bone marrow transplantation, the incidence of graft versus host disease (GVHD) after allogeneic bone marrow transplantation The morbidity and mortality rate are constantly increasing. The occurrence of GVHD is mainly due to the difference in immunogenetics between the donor and the recipient in addition to the consistency of the major histocompatibility antigen. The donor lymphocytes (mainly T lymphocytes) The resulting immune attack on the recipient severely affects the quality of life of the patient and is the leading cause of non-relaps...

Claims

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Application Information

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IPC IPC(8): A61L27/38
Inventor 项鹏张金丽
Owner SUN YAT SEN UNIV
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