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Surface activity liquid of lung

A technology of lung surfactant and clear liquid, which is applied in the direction of microbiology, biochemical equipment and methods, tissue culture, etc., and can solve problems that affect experimental results, complex serum components, and cell dedifferentiation

Inactive Publication Date: 2008-09-24
TIANJIN ZHENGJIANG HIGH TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the complex composition of serum, especially in some basic theoretical research, it often affects the experimental results
At the same time, serum also contains certain cytotoxic substances and inhibitory substances, which have a dedifferentiation effect on cells and affect the expression of cell functions.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0017] Pulmonary surfactant is a new biological product that has been used in production and has been researched by our unit through small and pilot tests for more than ten years. At present, only our unit produces exclusively in the whole country.

[0018] Lung surfactant is selected from healthy black-and-white bulls who have not eaten colostrum for less than 8 hours after birth. It is a lung surfactant extracted from healthy bulls with low antibodies.

[0019] 1. Main raw materials: selected healthy black-and-white bulls without colostrum.

[0020] Excipients: Sterilized normal saline injection, sterilizing and disinfecting drugs.

[0021] 2. Production method

[0022] Main production process: Extracted according to the aseptic extraction method in the "Operational Regulations for Biological Products of China".

[0023] 1. Main technical route

[0024] Aseptically collected fresh bovine lungs—wash repeatedly—obtain washing solution—centrifuge (2000 rpm, 20 minutes)—obta...

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Abstract

The invention provides a main manufacturing process of pulmonary surface active liquid, which comprises repetitively scrubbing a fresh aseptic ox lung to get cleaning mixture; centrifugating the cleaning mixture to get clear solution; sterilizing and filtering the clear solution to get the pulmonary surface active liquid; and freezing and storing the pulmonary surface active liquid. The pulmonary surface active liquid is a serum-free culture medium, and is rich in nutrient substance including macromolecular protein, nucleic acid and so on. Partial pulmonary surface active liquid can be added to substitute for serum during the growth and proliferation of animal cells. According to experiments, the pulmonary surface active liquid plays a significant role in the attachment and protection of cells and can neutralize the toxicity of toxic substance so that the cells are not injured. The pulmonary surface active liquid is used for experiment and production by tens of research academies and production units throughout China. The product is reliable in quality, and stable in nature. For instance, the doubling time of cells Sp2 / 0-Ag14 does not exceed 15 hours in supporting proliferation of cells; the doubling time is 20 hours when serum is used as a culture medium.

Description

technical field [0001] The invention belongs to biological products, in particular to a lung surfactant liquid. Background technique [0002] At present, the in vitro culture of most animal cells depends on serum to varying degrees. In addition to providing nutrients for cells, serum can also prompt cells to start synthesizing DNA, which has a great effect on cell proliferation. Experiments have shown that increasing the concentration of serum increases the maximum density of cell growth. Some recent studies have shown that the role of serum in culturing cells in vitro is mainly to provide growth factors necessary for cell proliferation. However, due to the complex composition of serum, especially in some basic theoretical research, it often affects the experimental results. At the same time, serum also contains certain cytotoxic substances and inhibitory substances, which have a dedifferentiation effect on cells and affect the expression of cell functions. In particular...

Claims

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Application Information

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IPC IPC(8): C12N5/06C12N5/07
Inventor 康志强陈燕
Owner TIANJIN ZHENGJIANG HIGH TECH
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