Syphilis spirochete membrane antigen with shortened expression and uses thereof
An expression vector and syphilis technology, applied in the field of genetic engineering, can solve the problems of increasing the specificity of detection reagents, blood waste, false positives, etc., and achieve the effect of preventing false positive results and avoiding cross-reactions
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[0040] Truncated Expression of TpN47 Membrane Antigen of Treponema pallidum
[0041] Truncated and expressed Treponema pallidum TpN47 membrane protein antigen, cloned and expressed Treponema pallidum standard strain (Nichols strain) by prokaryotic expression system, cloned and expressed TpN47 membrane protein gene open reading frame 5' end 1029bp sequence by prokaryotic expression system, and the expressed product was purified The TpN47 gene was truncated to retain the 1029bp part of the 5' end of the open reading frame, and was expressed in Escherichia coli.
[0042]Preparation of TP genome DNA: Treponema pallidum Nichols strain was provided by the Academy of Military Medical Sciences, and the extraction of TP genome was carried out according to the instructions of Beijing Tianwei Times Technology Co., Ltd. Bacterial Genome Extraction Kit.
[0043] According to the TpN47 gene sequence (gi: 6626258) published by GenBank, use the biological software PrimerPremier V5.0 to design...
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