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Syphilis spirochete membrane antigen with shortened expression and uses thereof

An expression vector and syphilis technology, applied in the field of genetic engineering, can solve the problems of increasing the specificity of detection reagents, blood waste, false positives, etc., and achieve the effect of preventing false positive results and avoiding cross-reactions

Inactive Publication Date: 2011-08-17
ARMY MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

It has been found that a sequence (411PGTEYT416) in TpN47 has high homology with human fibronectin, which may be related to the cross-reaction between some autoimmune patient serum and syphilis detection reagents (Baughn RE, Jiang A, Abraham R, et al. Molecular mimicry between an immunodominant amino acid motif on the 47-kDa lipoprotein of Treponema pallidum (Tpp47) and multiple repeats of analogous sequences infibronectin [J]. J Immunol, 1996, 157 (2): 720-731) this cross-reaction Sexuality can lead to false positives, clinical misdiagnosis, and serious consequences for the patient's body and mind and even the entire family
Once the blood donor sample is positive for syphilis, it will be eliminated, which will inevitably cause a certain amount of blood waste and bring a certain amount of mental pressure to the blood donor. Therefore, increasing the specificity of the detection reagent is very important to avoid false positives.

Method used

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  • Syphilis spirochete membrane antigen with shortened expression and uses thereof
  • Syphilis spirochete membrane antigen with shortened expression and uses thereof
  • Syphilis spirochete membrane antigen with shortened expression and uses thereof

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Embodiment Construction

[0040] Truncated Expression of TpN47 Membrane Antigen of Treponema pallidum

[0041] Truncated and expressed Treponema pallidum TpN47 membrane protein antigen, cloned and expressed Treponema pallidum standard strain (Nichols strain) by prokaryotic expression system, cloned and expressed TpN47 membrane protein gene open reading frame 5' end 1029bp sequence by prokaryotic expression system, and the expressed product was purified The TpN47 gene was truncated to retain the 1029bp part of the 5' end of the open reading frame, and was expressed in Escherichia coli.

[0042]Preparation of TP genome DNA: Treponema pallidum Nichols strain was provided by the Academy of Military Medical Sciences, and the extraction of TP genome was carried out according to the instructions of Beijing Tianwei Times Technology Co., Ltd. Bacterial Genome Extraction Kit.

[0043] According to the TpN47 gene sequence (gi: 6626258) published by GenBank, use the biological software PrimerPremier V5.0 to design...

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Abstract

The invention discloses a DNA sequence expressing a truncated treponema pallidum membrane antigen and an amino acid sequence The membrane antigen is removed of the part having high homology with human fibronectin, so as to avoid false positive and improve the specificity of the serological test for diagnosis of treponema pallidum infection. The invention also discloses the application of the membrane antigen in preparing diagnostic reagents for detecting treponema pallidum infection.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to an antigen fragment for diagnosing Treponema pallidum infection and its application. Background technique [0002] Syphilis is a sexually transmitted disease caused by Treponema pallidum. It has a long course after illness. It invades the genitals and skin in the early stage, and invades all organs of the body in the late stage. The lesions can almost affect all organs of the body, so it is extremely harmful. Syphilis can be divided into congenital syphilis and acquired syphilis according to the route of transmission. The disease was introduced into my country in the 16th century, and syphilis was once eliminated in China after the founding of the People's Republic of my country. In recent years, with the increasing frequency of foreign exchanges, the disease has resurged in our country, and the incidence rate is increasing year by year, which should be p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/20C12N15/31C12N15/63C12N1/21G01N33/68
Inventor 郭刚王宪灵邹全明石云张卫军蒲瑞雪
Owner ARMY MEDICAL UNIV
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