Unlock instant, AI-driven research and patent intelligence for your innovation.
Early detection method for arch insect infection
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology for early detection and toxoplasma gondii, applied in measuring devices, biological tests, material inspection products, etc., can solve problems such as severe fetal damage
Inactive Publication Date: 2008-12-03
WENZHOU MEDICAL UNIV
View PDF0 Cites 7 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
The degree of fetal damage is negatively correlated with gestational age. The earlier the infection occurs, the more severe the fetal damage will be. At present, there is no effective method for early diagnosis of toxoplasmosis
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0028] The preparation method of the fusion protein NTPase-II of Toxoplasma gondii in this detection method comprises the following steps:
[0029] (1) Preparation of Toxoplasma gondii genomic DNA;
[0030] (2) Amplification of the Toxoplasma gondii NTPase-II gene;
[0033] (5) transferring the recombinant obtained in (4) into a host cell;
[0034] (6) Induced expression of fusion protein NTPase-II;
[0035] (7) Separating and purifying the product of (5) to obtain NTPase-II expression protein.
[0036] Concrete preparation process is as follows:
[0037] (1) Preparation of Toxoplasma gondii genomic DNA:
[0038] The tachyzoites of Toxoplasma gondii RH strain were continuously passaged in mice, and the ascites was aseptically extracted 3 days after the infection, the parasites were washed with normal saline for 3 times, the ...
Embodiment 2
[0076] Dilute the purified NTPase-II with carbonic acid buffer to make a coating solution of 5 μg / ml, coat 100 μl / well on a 96-well ELISA plate, and coat overnight at 4°C; wash the plate 3-5 times with PBST, usually 3 times. Yes, 2-3min / time, pat dry; add 5% skimmed milkpowder, seal at 37°C for 2h; PBST wash plate 3-5 times, usually 5 times, 2-3min / time, pat dry; add dilution of 1:200 serum of the above-mentioned tested mice, 100 μl / well, incubate at 37°C for 1.5 h; wash the plate with PBST 5-7 times, preferably 7 times, 2-3 min each time, and pat dry; the dilution ratio is 1: 4 000 goat anti-mouse enzyme-labeled secondary antibody IgG, 100μl / well, incubate at 37°C for 1h, wash the plate with PBST 5-7 times, preferably 7 times, 2-3min / time, pat dry; add substrate chromogenic solution Solution A and B, develop color at 37°C in the dark for 10-15 minutes, add stop solution 2mol / L concentrated H 2 SO 4 Terminate the reaction, 50 μl / well; use a microplate reader to detect the a...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The invention relates to a method for detecting toxoplasma infection early. The nucleoside triphosphate hydrolase-II (NTPase-II) of toxoplasma is an indispensable key enzyme in the process that purine is remedied and synthesized by the toxoplasma by utilizing host cells, play an important part in parasitism and propagation of the toxoplasma, and is a major antigen for causing a body to generate immune reaction in the acute infection stage of the toxoplasma. The gene of the toxoplasma nucleoside triphosphate hydrolase-II is expanded by utilizing a polymerasechain reaction through the invention, expression plasmids are constructed and recombined in a expression vector, the recombined expression plasmids are transferred to procaryotic cells for expression, recombined fusion protein NTPase-II is obtained, the recombined fusion protein NTPase-II is used as an indirect ELISA method to establish a coatingantigen, and is used as the method for detecting the toxoplasma infection early.
Description
technical field [0001] The invention relates to a detection method of obligateintracellular parasitic opportunistic pathogenic protozoa, in particular to a method for early detection of toxoplasma gondii infection. Background technique [0002] Toxoplasma gondii (Toxoplasma gondii) is an obligateintracellular parasitic opportunistic pathogenic protozoan, which parasitizes and reproduces in the nucleated cells of humans and animals, causing toxoplasmosis that is zoonotic. Toxoplasmosis is distributed worldwide, and the average infection rate of human Toxoplasma is 33%. Healthy adults generally do not show obvious clinical symptoms after infection, but they can persist in the body for many years. When the body's immunity is weakened or severely infected, this latent infection can show significant symptoms of toxoplasmosis, such as toxoplasma eye disease, toxoplasma hepatitis, toxoplasma lymphadenitis, etc. Toxoplasma gondii infection often presents as a fatal lesion in imm...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More