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Method for preparing 5-hydroxytryptamine from hippophae rhamnoides

A technology of serotonin hydrochloride and seabuckthorn, applied in the field of preparation of 5-hydroxytryptamine, to achieve the effect of reducing production and extraction costs, simple and easy process, and rigorous process

Inactive Publication Date: 2011-04-13
QINGHAI TSINGHUA BIOTRY BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method for preparing 5-hydroxytryptamine from seabuckthorn branches, pericarp and pomace whose dry weight accounts for 12.41% of seabuckthorn branches has not yet been recorded

Method used

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  • Method for preparing 5-hydroxytryptamine from hippophae rhamnoides
  • Method for preparing 5-hydroxytryptamine from hippophae rhamnoides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1) Harvesting and cleaning of sea-buckthorn fruit branches: The collected fruit-bearing sea-buckthorn branches are less than 5cm in length and less than 6cm in diameter. Green fruits, diseased fruits, mud berries, rocks, metal utensils and other hard objects and foreign objects are removed; collected seabuckthorn The fruit must be packed in wooden or bamboo containers; the cleaning water must comply with GB5749, the processing environment must be clean and hygienic, try to eliminate the occurrence and reproduction of harmful organisms, and prevent harmful organisms from contacting the processing and processing equipment;

[0041] 2) Juice extraction: Use a PK-121R centrifuge to centrifuge at 3500 rpm for 8 minutes to obtain clear juice. After the clear juice is collected, it is used to produce sea buckthorn juice as raw materials for other products, such as sea buckthorn beverage, sea buckthorn wine, sea buckthorn oral liquid , Sea buckthorn fruit powder, sea buckthorn vita...

Embodiment 2

[0052] 1) Harvesting and cleaning of sea-buckthorn fruit branches: The collected fruit-bearing sea-buckthorn branches are less than 5cm in length and less than 6cm in diameter. Green fruits, diseased fruits, mud berries, rocks, metal utensils and other hard objects and foreign objects are removed; collected seabuckthorn The fruit must be packed in wooden or bamboo containers; the cleaning water must meet the requirements of GB5749; the processing environment must be clean and hygienic, try to eliminate the occurrence and reproduction of harmful organisms, and prevent harmful organisms from contacting processing and processing equipment;

[0053] 2) Juice extraction: use a PK-121R centrifuge to centrifuge at 4000 rpm for 6 minutes to obtain clear juice. After the clear juice is collected, it is used to produce sea buckthorn juice as raw materials in other countries, such as sea buckthorn beverages, sea buckthorn wine, sea buckthorn oral liquid , Sea buckthorn fruit powder, sea buck...

Embodiment 3

[0064] 1) Harvesting and cleaning of sea-buckthorn fruit branches: The collected fruit-bearing sea-buckthorn branches are less than 5cm in length and less than 6cm in diameter. Green fruits, diseased fruits, mud berries, rocks, metal utensils and other hard objects and foreign objects are removed; collected seabuckthorn The fruit must be packed in wooden or bamboo containers; the cleaning water must meet the requirements of GB5749; the processing environment must be clean and hygienic, try to eliminate the occurrence and reproduction of harmful organisms, and prevent harmful organisms from contacting processing and processing equipment;

[0065] 2) Juice extraction: use a PK-121R centrifuge at 4500 rpm for 4 minutes to separate and obtain clear juice. After the clear juice is collected, it is sea buckthorn juice, which is used to produce sea buckthorn juice as raw materials for other products, such as sea buckthorn drinks and sea buckthorn Wine, sea buckthorn oral liquid, sea buck...

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Abstract

The invention relates to a method for preparing natural active substance mixture from tresses, pomaces and peels of a plant, in particular to a method for preparing 5-hydroxytryptamine from sea buckthorn. The method for preparing 5-hydroxytryptamine from sea buckthorn comprises the following steps: (1) the tresses of a sea buckthorn fruit is collected and washed; (2) juice is extracted; (3) separation is performed; (4) water extraction is performed; (5) filtration is performed; (6) ion exchange is performed; (7) washing and elution are performed; (8) acid reduction is performed; (9) concentration is performed; (10) extraction is performed by ethanol; (11) the product is obtained through crystallization. The method for extracting 5-hydroxytryptamine from the tresses, the pomaces and the peels of sea buckthorn, and the waste can be reused; 5-hydroxytryptamine has high content and can be used as raw material for waste utilization, and the manufacturing extraction cost of 5-hydroxytryptamine is lowered. The method for preparing 5-hydroxytryptamine from sea buckthorn has simple and easy technology, scientific and reasonable processes, precise working procedure, low cost, and high product purity.

Description

Technical field [0001] The invention relates to a method for preparing a mixture of natural active substances from plant branches, pomace and pericarp, in particular to a method for preparing serotonin from sea buckthorn. Background technique [0002] Serotonin (5-hydroxytryptamine, 5-HT), also known as serotoni, was first isolated from serum by Rapport et al. (1941) and named serotonin. Soon, Erspamer and Boretti (1951) discovered intestinal amine from the chromaffin cells of the intestinal mucosa, and later confirmed that the two are the same compound. Serotonin can react with acid to form a crystalline salt. Its hydrochloride has a melting point of 167-168°C, and picric acid has a melting point of 185-189°C. The concentration of serotonin in brain tissue is relatively high. It is an important substance that regulates nerve activity and participates in the regulation of physiological functions such as pain, sleep and body temperature. Serotonin can help sleep, so serotonin is ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D209/16
Inventor 鲁长征山永凯刘洪智李树志
Owner QINGHAI TSINGHUA BIOTRY BIO TECH
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