Magnetic particle for separation as well as preparation method and application thereof

A magnetic particle and magnetic core technology, which is applied in the fields of magnetism of inorganic materials, analytical materials, dyeing polymer organic compounds, etc., can solve the problems of target protein degradation, cell damage, slow separation speed, and low labor efficiency

Active Publication Date: 2009-01-28
胡涵晶
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although the advantages of magnetic particles have been recognized, but because the current commercially available magnetic particles are all made of Fe 3 o 4 and Fe 2 o 3 Composed of mixtures, the separation speed is slow and cannot meet people's requirements
The slow separation speed not only reduces the labor efficiency and wastes time, but also leads to the degradation of the separated target protein and the damage of the separated cells, especially for stem cells, circulating tumor cells, rare immune cells and other cells that need to be further cultured. so so

Method used

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  • Magnetic particle for separation as well as preparation method and application thereof
  • Magnetic particle for separation as well as preparation method and application thereof
  • Magnetic particle for separation as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0039] Embodiment 1, the preparation of the magnetic particle of the present invention

[0040] Prepare Fe with a molar ratio of 1:1:0.3 2 o 3 : Fe 3 o 4 :TiO 2 Aqueous iron solution. In the presence of 1.5M ammonium hydroxide or sodium hydroxide, the aqueous iron solution was mixed to form a superparamagnetic iron oxide precipitate. The precipitate (ie colloid) was washed with water to pH 7.2 and then washed again in NaCl to maintain the fineness of the iron oxide crystals. Finally, the prepared colloids were stored in deionized water. Subsequently, 25 ml of the colloidal slurry was incubated with 50% by volume of perchloric acid to colloidize the entire magnetic core by a magnetic field. No matter how many times a magnetic field is applied to the particle, the particle does not become permanently magnetized. A 10% benzyl peroxide solution was added to 40 ml of colloid and incubated at 65° C. for 24 hours, followed by washing with deionized water. The magnetic part...

Embodiment 2

[0041] Example 2, preparation of magnetic particles of the present invention coated with ethylene oxide layer and dextran

[0042] For the ethylene oxide coating, 1 g of the magnetic core prepared in Example 1 was mixed with 8 ml of allyl glycidyl ether, 3 ml of divinylbenzene and 0.1 g of K 2 S 2 o 8 Incubate at 50°C for 3 hours, then wash with H 2 O washing.

[0043] For dextran coating, the magnetic particles were resuspended in β-divinylbenzene to a concentration of 20% and shaken continuously at 65°C for 24 hours. After equilibrating the pressure to atmospheric pressure, the solution was sonicated with an ordinary water-bath sonicator at an output of 75% for 2 minutes, and then washed with water several times. The supernatant was discarded, and the magnetic particle pellet was incubated with 12% dextran solution at 42°C for 16 hours. The magnetic particles were washed with water several times, and the prepared dextran-coated magnetic particles were stored in water ...

Embodiment 3

[0045] Example 3. Preparation of magnetic particles of the present invention containing oxidized dextran

[0046] With the magnetic particle prepared in 50g embodiment 2 and 0.01M NaIO 4 Incubate for 10 minutes. 50 g of the prepared oxidized dextran magnetic particles were washed with PBS (phosphate buffered saline), and resuspended in 100 ml of PBS.

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Abstract

The invention relates to a magnetic particle for separation, a preparation method and an application thereof, in particular to the magnetic particle containing TiO2 of certain molar ratio in a magnetic core, the method for preparing the same and the application. Compared with the prior art, the magnetic particle has rapider separation speed and can be used for separating microorganism, cells, protein, nucleic acid, a compound or other target materials, thereby being widely applied to the fields of biology, biomedicine, clinic and foodstuff.

Description

technical field [0001] The invention relates to the field of biological separation, in particular to magnetic particles for separation, a preparation method and application thereof. Background technique [0002] It is well known that magnetic separation has been successfully and widely used in biological and biomedical systems. Some advantages of various separations in terms of high-throughput and automation have been recognized. Magnetic particles (also called magnetic beads) facilitate the separation of bound from unbound analyte. Compared with centrifugation, which is time-consuming, costly, and radiologically, biologically, and physically hazardous after application, magnetic separation is fast and easy, requiring only simple equipment. In addition, magnetic separation provides better mass transfer and produces less fouling than traditional affinity chromatography. [0003] Although the advantages of magnetic particles have been recognized, but because the current com...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): H01F1/06B22F1/02C09C3/10G01N33/553
Inventor 赵海峰
Owner 胡涵晶
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