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Plant gene promoter and its use

A promoter and plant technology, applied in plant products, genetic engineering, botanical equipment and methods, etc., can solve problems such as temperature stress, pollen proteome less comprehensive analysis, etc.

Inactive Publication Date: 2009-06-24
STATE OF ISRAEL MINIST OF AGRI & RURAL DEV AGRI RES ORG (A R O) (VOLCANI CENT)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To date, only few comprehensive analyzes specific to the pollen proteome exist, and only one such analysis addresses temperature stress[1]

Method used

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  • Plant gene promoter and its use
  • Plant gene promoter and its use
  • Plant gene promoter and its use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0125] 1. The effect of HTS on pollen development and germination

[0126] The effect of HTS on pollen development and germination in figure 1 been confirmed in. Pollen from tomato plants grown at normal temperature (28 / 22°C day / night) had good viability after germination at 23°C and significantly reduced viability after germination at 32°C, pointing to the effect of HTS on pollen germination negative effects. Pollen from plants grown at high temperatures (32 / 36°C) also showed lower viability when germinated at 23°C, pointing to a persistent negative effect of HTS on pollen development.

[0127] 2. Overexpression of AtHXK1 improves the development and germination of tomato pollen under HTS

[0128] To investigate the importance of hexose phosphorylation in determining the germination ability of pollen, pollen germination was analyzed in tomato plants overexpressing the Arabidopsis hexokinase gene (AtHXK1) (35S::AtHXK1) under the control of the CaMV35S promoter [8]. Altho...

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PUM

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Abstract

A method for protecting a plant from high temperature stress (HTS), comprising transforming the plant with a polynucleotide sequence. The polynucleotide sequence comprises a nucleotide sequence encoding a sugar kinase under the control of an anther and / or pollen specific promoter, thereby producing a transformed plant having improved tolerance to HTS. Also disclosed is the polynucleotide sequence of the LeFRK4 promoter.

Description

field of invention [0001] The present invention relates to a plant gene promoter and its application. [0002] references [0003] The following references are mentioned in the specification and identified by their respective numbers in the text. Mention of a reference does not necessarily indicate that it is relevant to the patentability of the invention. [0004] 1. Imin N, Kerim T, Rolfe BG, Weinman JJ (2004) Effect of early cold stresson the maturation of rice anthers. Proteomics 4: 1873-1882; [0005] 2. Aloni B, Peet M, Pharr M, Karni L (2001) The effect of high temperature and high atmospheric CO2 on carbohydrate changes in bell pepper (Capsicum annuum) pollen in relation to its germination. Physiol Plant 112: 505-512; [0006] 3. Pressman E, Peet MM, Pharr DM (2002) The effect of heat stress on tomatopollen characteristics is associated with changes in carbohydrate concentration in the developing anthers. Ann Bot (Lond) 90: 631-636; [0007] 4. German MA, Asher I, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82C12N5/10A01H5/00
CPCC12N15/8289C12N15/8271C12N15/8273C12N15/8231
Inventor D·格拉诺特D·斯沃茨伯格M·杰曼
Owner STATE OF ISRAEL MINIST OF AGRI & RURAL DEV AGRI RES ORG (A R O) (VOLCANI CENT)
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