Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for inactivating hereditary substance of fish sperm using X ray and use thereof

A technology for fish sperm and genetic material, applied in the field of high-efficiency inactivation of fish sperm genetic material, can solve the problem that the optimal irradiation conditions and optimal irradiation dose of inactivated fish sperm are not determined, the lack of systematic and in-depth research, X The application of ray is stagnant and other problems, to achieve the effect of good inactivation effect, stable results and broad application prospects

Inactive Publication Date: 2011-05-11
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] According to literature reports, in 1913, X-rays were first used to process fish sperm. By the 1980s, the application of X-rays in fish mutagenesis breeding was also reported. Although it was found that X-rays can be used to inactivate and mutagenize genetic material , but all belong to preliminary qualitative research, lack of systematic and in-depth research, and have not determined the best irradiation conditions and the best irradiation dose for inactivating fish sperm
Especially since the 1980s, there has been no report on the application of X-rays to fish research, and the relevant research has stalled for nearly 30 years, resulting in the stagnation of the application of X-rays in fish.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for inactivating hereditary substance of fish sperm using X ray and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] 1. Select fish species

[0042] Select the male Jian carp with strong physique, no external damage, and the semen will flow out automatically when the abdomen is lightly squeezed.

[0043] 2. Collection of semen

[0044]First use a clean towel to dry the water around the genital opening of the male fish, gently squeeze the sides of the abdomen of the fish to force the semen to flow out, suck out 1.5ml of the semen with a dry and clean straw, and put it in a 1.5ml transparent polypropylene Eppendorf centrifuge tube. Semen requires no water, no blood, no urine, milky white, and it will disperse quickly when it meets water.

[0045] 3. Inactivation treatment of semen genetic material

[0046] Place the centrifuge tube in a petri dish filled with ice-water mixture, then put the petri dish into an X-ray irradiator for irradiation, the intensity is 100kV-5mA, the source distance is 5cm, and the irradiation time is 50 minutes. The irradiated semen was stored in a refrigerat...

Embodiment 2

[0049] 1. Select fish species

[0050] Choose a mature male goldfish with a strong physique, no external damage, and automatic semen flow out of the abdomen when lightly squeezed.

[0051] 2. Collection of semen

[0052] First use a clean towel to dry the water around the genital opening of the male fish, gently squeeze the sides of the abdomen of the fish to force the semen to flow out, suck out 0.5ml of the semen with a dry and clean straw, and put it in a 1.5ml transparent polypropylene Eppendorf centrifuge tube. Semen requires no water, no blood, no urine, and the milky white color will disperse quickly when it meets water.

[0053] 3. Inactivation treatment of semen genetic material

[0054] Place the centrifuge tube in a petri dish filled with a mixture of ice and water, then place the petri dish into an X-ray irradiator for irradiation with an intensity of 100kV-5mA, a source distance of 10cm, and an irradiation time of 60 minutes. The irradiated semen was stored in ...

Embodiment 3

[0057] 1. Select fish species

[0058] Choose a mature male koi with a strong physique, no external damage, and the semen will flow out automatically when the abdomen is lightly squeezed.

[0059] 2. Collection of semen

[0060] First use a clean towel to dry the water around the genital opening of the male fish, gently squeeze the sides of the abdomen of the fish to force the semen to flow out, suck out 1ml of the semen with a dry and clean straw, and put it in a 1.5ml transparent polypropylene Eppendorf centrifuge tube. Semen requires no water, no blood, no urine, and the milky white color will disperse quickly when it meets water.

[0061] 3. Inactivation treatment of semen genetic material

[0062] Place the centrifuge tube in a petri dish filled with a mixture of ice and water, then put the petri dish into an X-ray irradiator for irradiation with an intensity of 100kV-5mA, a source distance of 10cm, and an irradiation time of 70 minutes. The irradiated semen was stored...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
X-ray intensityaaaaaaaaaa
X-ray intensityaaaaaaaaaa
Login to View More

Abstract

The invention relates to a method of using X radial to kill sperm genetic materials of alive fishes which uses X radial to irradiate fish sperm collected by people. 0.5-1.5ml of fish sperm is put in a transparent polypropylene eppendorf centrifuge tube with volume of 1.5ml; intensity of the X radial is 100kV-5mA; radiating time is 50-80 minutes; radiating distance is 5-15cm. On the premise of preserving insemination function of sperm, the method realizes effectively killing sperm genetic materials of fishes. The invention also relates to the application of the method of using X radial to killsperm genetic materials of alive fishes to genetic breeding of freshwater fishes.

Description

technical field [0001] The invention relates to a method for fish artificial gynogenesis, in particular to a method for efficiently inactivating genetic material of fish sperm. Background technique [0002] Artificial induction of gynogenesis is one of the important ways of fish genetics and breeding. It can be used for fish sex control, pure line establishment, asexual reproduction and cloning, protection of endangered fish, gene positioning, etc., in genetic research and new species breeding have wide applications. Artificial induction of gynogenesis includes two core technologies, one is to inactivate the genetic material of the sperm under the premise of maintaining the fertilization function of the sperm, and the other is to use the genetically inactivated sperm to inseminate an egg with normal genetic material to obtain a fertilized egg , to double the genetic material (chromosome), in which how to efficiently inactivate the genetic material of the sperm is the key. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/00A61K35/52
Inventor 傅洪拓龚永生吴滟何新龙熊贻伟蒋速飞王晓娟
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com