Method for tissue separation of edible fungus

A technology for tissue separation and fungus, applied in botany equipment and methods, fungi, horticulture, etc., can solve the problems of low mycelium separation success rate, complicated operation, poor quality, etc., achieve material saving, high separation success rate, The effect of low production cost

Inactive Publication Date: 2009-07-08
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to overcome defects such as complicated operation, high cost, low hyphae separation success rate, and poor quality in the existing method for separating dry ear strains, and provide a simple operation with few materials (including medicine), low cost, fast mycelium germination, strong growth, dense, high separation success rate dry ear tissue separation method to solve a series of technical problems in the conventional separation method of fungus, and provide convenience for the majority of seed producers

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1 ear piece source

[0031] Black fungus collected from Dagang Mountain in Fenyi, Jiangxi, Diaoluo Mountain in Lingshui County, Hainan Province, Bawangling Mountain in Changjiang County, Hainan Province, Chongqing Normal University, Yellow Crane Tower Scenic Spot in Wuhan City, Beixin Village in Laishui County, Hebei Province, etc.[ Auricularia auricula (L.) Underw.] specimens were washed with clean water, and dried indoors naturally in the shade or in an oven at 28°C; after drying, the tissue isolation strains could be performed.

[0032] The common biological traits of black fungus specimens collected in the wild are as follows:

[0033] Growing on fallen and decayed wood of various broad-leaved trees; the fruiting bodies are annual, thin or thick, cup-shaped, auricle-shaped, leaf-shaped or petal-shaped when fresh, solitary or in clusters, brown to dark brown, soft and translucent , gelatinous, elastic, concave in the middle, sharp edges, 2-9cm in diameter, sometimes...

Embodiment 2

[0046] Ear piece source

[0047] Auricularia auricularia collected from Dagang Mountain in Fenyi, Jiangxi, Diaoluo Mountain in Lingshui County, Hainan Province, Bawangling Mountain in Changjiang County, Hainan Province, Chongqing Normal University, Yellow Crane Tower Scenic Spot in Wuhan City, Beixin Village in Laishui County, Hebei Province, etc.[ Auricularia polytricha (Mont.) Sacc.] specimens were washed with clean water, and dried indoors naturally in the shade or in an oven at 28°C; after drying, the strains could be isolated from tissues.

[0048] The common biological traits of the wild fungus specimens collected are as follows:

[0049] Grows on fallen and rotting wood of many broad-leaved trees. Fruiting bodies are annual, cup-shaped, saucer-shaped or auricle-shaped when fresh, thicker, usually clustered, sometimes solitary, brown to dark brown, gelatinous, slightly hard, sunken in the middle, sharp and usually upper Rolls, up to 15cm in diameter, 0.5-1.5mm thick. ...

Embodiment 3

[0054] Ear piece source

[0055] The wrinkled fungus ( Auricularia delicata (Fr.) Henn.) specimens were washed with clean water, and dried naturally in the shade indoors or in an oven at 28°C; after drying, the tissue isolation strains could be performed.

[0056] The common biological traits of the specimens of wrinkled fungus collected in the wild are as follows:

[0057] It mostly grows on dry and rotted broad-leaved trees such as Alder Poplar and Millennium Tong; the fruiting body is generally small, gelatinous, slightly tough, ear-shaped or disc-shaped, sessile, clustered, 1.5-7cm×1-4cm in diameter. Purple-brown or purple-red, darkening when dry. The surface of the hymenium is light reddish brown, with white powder, and has obvious wrinkles and forms a grid, and the outside is slightly wrinkled. The unblinded surface is dark chestnut brown to slightly purplish black, with dense short fine hairs.

[0058] The preparation process of culture medium is identical with embo...

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Abstract

The invention discloses a tissue isolation method of edible fungus strains. The method comprises the following steps: (1) sterilizing and moistening dried edible fungus; (2) cutting a fungus tissue block; (3) sterilizing the fungus tissue block with alcohol lamp flame; (4) inserting the fungus tissue block in an isolation culture medium for thermostatic isolating culture; and (5) selecting new hypha as an inoculation block to be inoculated to a purified culture medium for purification culture, and obtaining the edible fungus strains. The tissue isolation method of the edible fungus strains has the advantages of simple operation, low production cost, high hypha isolation success ratio, fast hypha germination, strong growth vigor and the like. The method has wide application, and is applicable to tissue isolation of various jelly fungi.

Description

technical field [0001] The invention relates to a method for separating edible fungus strains, in particular to a method for separating and purifying fungus strains by using a tissue separation method, and belongs to the field of separation and purification of edible fungus strains. Background technique [0002] my country has a vast territory, including tropical, subtropical, and temperate climate zones. Various terrains, mild and humid conditions are suitable for growing many kinds of fungus. Northeast, central and south are the main growth areas of fungus in my country. Black fungus [Auricularia auricula (L.) Underw.] and fungus [A.polytricha (Mont.) Sacc.] is the most widely distributed species with the highest yield. [0003] Black fungus is a traditional food that our people like, and it is an indispensable condiment for cooking high-end dishes. The black fungus is delicate in texture and rich in nutrition. It contains a variety of amino acids necessary for the human ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C12N1/14
Inventor 崔宝凯杜萍戴玉成
Owner BEIJING FORESTRY UNIVERSITY
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