The invention relates to a method for separating wheat stem base rot
bacteria. The method comprises the following steps of firstly collecting wheat plants, spreading out and standing for 1-2 days under the ventilated and shade conditions, and
cutting a stem of which the length is 1.8-2.2cm from the base where the first and second stem nodes become brown; rinsing with
clean water, then sterilizing with 75%
alcohol for 15 seconds, and then rinsing with
sterile water; then placing in a culture dish of which the bottom is covered with sterile
filter paper and adding 1-2mL of
sterile water, then placing in a biochemical
incubator, and culturing at 25 DEG C under a dark condition until mycelia grow on stems; picking mycelia, inoculating the mycelia on a PSA medium added with
lactic acid, and carrying out dark culture in the biochemical
incubator of which the temperature is 25 DEG C; picking a small
bacterial colony from the edge of a
bacterial colony with an
inoculation needle, transferring the small
bacterial colony onto the PSA slant culture medium, placing in the biochemical
incubator, culturing at 25 DEG C under a dark condition for 3 days and carrying out strain preservation, namely, separating the wheat stem base rot
bacteria out. The method disclosed by the invention has the
advantage of high success rate of separation, the mycelia can quickly grow from the treated stem, typical colonies of the wheat stem base rot
bacteria can quickly grow after the mycelia are transferred to the PSA and the success rate reaches up to above 95%.