40results about How to "Improve the success rate of separation" patented technology

The invention provides a peeling device and a peeling method for a display substrate. The peeling device for the display substrate comprises a base board, at least one adhesive plate, a detection unit and a separation unit, wherein each adhesive plate is arranged in a region, which corresponds to the corner or edge of the display substrate, of the base board, and is connected with the base board in a rotatable manner, so that the upper surface of each adhesive plate and the upper surface of the base board are rotated in a coplanar state to the upper surface of the base board to form a non-coplanar state; the detection unit is used for detecting whether the edge region of the display substrate is adhered to the adhesive plate; the separation unit is arranged above the base board and the adhesive plate and comprises a first adsorption terminal and a second adsorption terminal which are capable of moving in the vertical direction; at least one first adsorption terminal is correspondingly arranged above the adhesive plate; a plurality of second adsorption terminals are correspondingly arranged above the base board; projections of the multiple second adsorption terminals on the base board are arranged in sequence along the region away from the corner or edge of the adhesive plate.

The invention provides a method for separating nano particles of different particle sizes. According to the method, nano powder is fully dispersed into solvent of certain viscosity, and the nano particles which are of different particle sizes and are narrow in particle size distribution range can be obtained under the centrifugal effect at different rotation speeds. The method is convenient and easy to implement, the operation is easy, the nano powder which is narrow in particle size distribution range can be obtained without adjusting the density of the solvent in a mixed system, and the success rate of separation is high.

The invention discloses a tissue isolation method of edible fungus strains. The method comprises the following steps: (1) sterilizing and moistening dried edible fungus; (2) cutting a fungus tissue block; (3) sterilizing the fungus tissue block with alcohol lamp flame; (4) inserting the fungus tissue block in an isolation culture medium for thermostatic isolating culture; and (5) selecting new hypha as an inoculation block to be inoculated to a purified culture medium for purification culture, and obtaining the edible fungus strains. The tissue isolation method of the edible fungus strains has the advantages of simple operation, low production cost, high hypha isolation success ratio, fast hypha germination, strong growth vigor and the like. The method has wide application, and is applicable to tissue isolation of various jelly fungi.

A low-temperature wafer bonding machine and a wafer bonding method are provided. The low-temperature wafer bonding machine comprises a surface treatment unit used for activating the surfaces of to-be-bonded wafers, a pre-bonding unit used for bonding one activated to-be-bonded wafer to at least one other activated to-be-bonded wafer or at least one other unactivated to-be-bonded wafer to form a pre-bonded wafer pair, a missing detection unit used for detecting whether the pre-bonded wafer pair is missing, and a de-bonding unit used for separating the missing pre-bonded wafer pair. The successrate of de-bonding of the low-temperature wafer bonding machine of the invention is improved.

The invention provides a combined type toy gyro separated through induction control. The combined type toy gyro comprises a main gyro body, an auxiliary gyro body and an elastic part. When the combined type toy gyro is in a first state, the main gyro body is limited and fixed above the auxiliary gyro body through the auxiliary gyro body, and meanwhile the main gyro body compresses the elastic part so that the elastic part can be compressed. The combined type toy gyro is characterized in that a sensing mechanism is arranged in the auxiliary gyro body, the sensing mechanism can controllably relieve limiting fixation of the main gyro body from the auxiliary gyro body under a second state through induction control, the main gyro body pops up under the elasticity action of the elastic part, and accordingly the main gyro body and the auxiliary gyro body rotate independently. Thus, players can separate the gyro through induction at will; meanwhile, because of inductive separation, rotation of the toy gyro cannot be influenced, the whole game process has controllability, operability is high, playing methods are more novel, interestingness is higher, the gyro is divided into two parts, attack force of gyros of own sides is greatly increased, the win rate is higher, and the gyro can be popular with more players.

The invention belongs to the technical field of veterinary biology, and relates to a mycoplasma hyopneumoniae selective separation medium and a preparation method thereof. The mycoplasma hyopneumoniae selective separation medium comprises the main components of lactoalbumin hydrolysate, yeast extract powder, MEM (minimum essential medium), Hank 's solution, 0.1% phenol red solution, healthy horse serum, penicillin, deionized water, and specific growth inhibition serum. The mycoplasma hyopneumoniae selective separation medium can be used for the rapid separation and identification of mycoplasma hyopneumoniae, the preparation method is simple and feasible, experimental results are reliable, the separation time is shortened to 103h-122h, the time is shortened to 17%-23%, the separation success rate is as high as 75% above, and is more than 10 times of the separation success rate of mediums in the prior art.

The invention relates to a porcine epidemic diarrhea virus isolated culture method which comprises the following steps: thoroughly rinsing small intestine of a piglet, culturing the small intestine in a culture medium, inoculating, diluting a PEDV (Porcine Epidemic Diarrhea Virus) sample which is positive through detection into a suspension with the volume ratio of 1:1 by using normal saline, culturing and harvesting cells with the cytopathic effect (CPE), continuously freeze-thawing and inoculating single-layer vero cells, culturing by using the culture medium to obtain the cells with the cytopathic effect (CPE), namely, separating to obtain the porcine epidemic diarrhea virus. Compared with the prior art, the method is high in separation success rate and the separated porcine epidemic diarrhea virus is high in virulence and high in purity.

Belonging to the field of veterinary biological products, the invention discloses a mycoplaa hyopneumoniae selective isolation medium and a preparation method thereof. The mycoplaa hyopneumoniae selective isolation medium comprises the following components: brain heart infusion, PPLO broth, 10*Hank's solution, yeast extracted liquid, lactalbumin hydrolysate, an amino acid composition, glucose, penicillin, nystatin, 0.1% phenol red, inactivated pig serum, specific growth inhibiting serum and deionized water. The mycoplaa hyopneumoniae selective isolation medium provided by the invention has thecharacteristics of short isolation time, high isolation success rate, viable bacteria titer and isolation sensitivity far higher than those of the medium in the prior art, and is suitable for rapid growth of mycoplaa hyopneumoniae.

The invention provides a membrane separation device which comprises a bearing table, a separation mechanism and a collection mechanism. The bearing table comprises a bearing surface, wherein the bearing surface is used for adsorbing a substrate of a diaphragm, and the collecting mechanism comprises a collecting surface; the bearing surface and the collecting surface are arranged on the two sides of the separating mechanism correspondingly. The bearing surface is opposite to the separation mechanism; the collecting surface is opposite to the separating mechanism, the separating mechanism comprises a separating roller, the separating roller can rotate around the axis of the separating roller and can move relative to the bearing table at the same time, a plurality of first outer air holes areformed in the outer surface of the separating roller, and the multiple first outer air holes can suck air or spray air respectively. The invention further provides a membrane separation method. According to the membrane separation device, the functional layer of the body of the membrane can be prevented from being damaged or polluted in the separation process.

The invention discloses a multifunctional feeder of a separator. A discharging pipe is connected with a hopper; supporting legs are mounted at the bottom of the hopper; a feeding screw is arranged inside the hopper and is driven by a driving mechanism to rotate; an opening is provided with a filter screen of which the middle is provided with a grate with the mesh being smaller than that of the filter screen in a covering way; a water pipe support is arranged at a corner, and the top end is provided with water spray nozzles spraying water towards the grate; each side wall of the hopper is also provided with a water delivery pipe of which one end communicates with the hopper through a water pump and the other end is connected with one of the water spray nozzles; screw blades on the feeding screw are provided with a plurality of groups of metal brushes; the edge parts of the opening vertically and upwards extend to form a rear baffle and two side plates, each side plate is provided with a telescopic cylinder of which the head of a telescopic rod is provided with an extrusion plate, and the two extrusion plates can be close to or apart away from each other; and a plurality of extrusion teeth are formed on the extrusion plates. Sand adhered to stone blocks can be washed before concrete mortar enters a sand and stone separator, the separation effect can be improved, meanwhile, the washing water can be saved, waste can be avoided, and massive materials can also be smashed and recovered.

The invention relates to a method for fast separating microbes in Puer ripe tea, in particular to a method for fast separating cleistothecium (commonly referred to as golden flowers) generating microbes in the Puer ripe tea, which belongs to the technical field of the separation culture of beneficial microbes fermented by the Puer ripe tea. According to the analysis on the golden flower occurrence environment condition, a separation culture medium is determined by an orthogonal optimization experimental method, and then, the basic culture condition is determined after the further comparison perfection and the comparison study on the culture condition, so the bacterial colony of cleistothecium generating bacteria on a flat plate is fast expanded, the bacterial colony is large, the aerial hypha growth is good, the asexual stage is shortened, the generation of yellow ascocarp cleistothecium (commonly referring to the golden flowers) of sexual multiplication structures is promoted, and the goals and requirements of fast separation and culture multiplication are reached. The method comprises the steps of culture medium preparation, flat plate separation culture condition determination, tea sample separation and microbe determination. The method has the characteristics of high speed, simplicity, high pertinence and high separation success rate.

The invention discloses a culture method of mesenchymal stem cells contained in umbilical cord blood, belongs to the technical field of stem cell culture, and solves the problems that the success rate of separation of umbilical cord blood mesenchymal stem cells is low and the cell proliferation rate is low in the culture process in the prior art. The culture method specifically includes the following steps: step S1, collecting and transporting umbilical cord blood; step S2, extracting the mesenchymal stem cells; step S3, performing primary culture; step S4, performing subculture; and step S5, performing cryopreservation. According to the culture method, firstly, effective umbilical cord blood is acquired, sedimentation of red blood cells is accelerated by adding macromolecular hydroxyethyl starch with the combination of a density gradient centrifugation method, the success rate of separation of a single mesenchymal stem cell is increased; a specially-made culture medium used for the primary culture contains astragalus polysaccharide, and the astragalus polysaccharide can also maintain the phenotype and characteristics of the umbilical cord blood mesenchymal stem cells while promoting cell proliferation; and the culture method provided by the invention is simple to operate, free of exogenous pollution, safe and reliable and high in cell proliferation rate.

The invention discloses a laser separation machining method and a laser separation machining device for optical crystal. The method comprises the following steps of: simply roughly rubbing and polishing the convex-concave surface of the optical crystal along a laser separation machining locus, and cleaning a hydrolysis atomization layer and impurities on the surface to form a transparent locus of which the width is greater than the diameter of a laser incident light spot; uniformly coating a saturated solution of the same optical crystal material on the surface of the wiped optical crystal to fill the convex-concave part of the surface of the optical crystal; and performing scanning separation machining by adopting laser along the locus of the saturated solution of the optical crystal. The device comprises a laser machining system and a solution coating system; and a laser focusing device, a solution coating device, a wiping device, a polishing device and a polishing device are sequentially arranged on a linear moving mechanism along the X-axis direction. By the method and the device, a non-fragmented optical crystal sheet with high separation precision, abrupt separation machining port, smooth cut separation surface and high separation quality can be obtained, and negative effects caused by over high laser temperature can be effectively inhibited.

A robot end actuator used for picking clustered tomatoes includes an outer cylinder, an inner cylinder, a clustered fruit collecting mechanism, a fruit releasing mechanism, N fruit stem rotating and cutting mechanisms and the like. The clustered fruit collecting mechanism is controlled through a limiting switch and a time delay module, a direct current deceleration motor and a direct current push-pull electromagnet are sequentially electrified and rotate, a rotating disc is driven to rotate so that several fruit stems of the clustered tomatoes are gathered and tightened, and then the fruit stems are cut off through the fruit stem rotating and cutting mechanisms. The fruit releasing mechanism can fix the outer cylinder and a handle, and then through rotation of a mechanical arm, the tomatoes can be released out from the inner cylinder. A cluster of several tomatoes on one plant can be harvested simultaneously through a single operation task, the picking efficiency of the robot is improved, and rotation of the rotating disc makes the several fruit stems of the clustered tomatoes gathered and tightened so that the high-speed circular motion of cutting blades can effectively improve the success rate of separation of fruit stems for the rotating and cutting device.

The invention relates to a method for separating wheat stem base rot bacteria. The method comprises the following steps of firstly collecting wheat plants, spreading out and standing for 1-2 days under the ventilated and shade conditions, and cutting a stem of which the length is 1.8-2.2cm from the base where the first and second stem nodes become brown; rinsing with clean water, then sterilizing with 75% alcohol for 15 seconds, and then rinsing with sterile water; then placing in a culture dish of which the bottom is covered with sterile filter paper and adding 1-2mL of sterile water, then placing in a biochemical incubator, and culturing at 25 DEG C under a dark condition until mycelia grow on stems; picking mycelia, inoculating the mycelia on a PSA medium added with lactic acid, and carrying out dark culture in the biochemical incubator of which the temperature is 25 DEG C; picking a small bacterial colony from the edge of a bacterial colony with an inoculation needle, transferring the small bacterial colony onto the PSA slant culture medium, placing in the biochemical incubator, culturing at 25 DEG C under a dark condition for 3 days and carrying out strain preservation, namely, separating the wheat stem base rot bacteria out. The method disclosed by the invention has the advantage of high success rate of separation, the mycelia can quickly grow from the treated stem, typical colonies of the wheat stem base rot bacteria can quickly grow after the mycelia are transferred to the PSA and the success rate reaches up to above 95%.

The invention discloses a semiconductor substrate and a method for separating a substrate layer from a functional layer on the substrate layer. The semiconductor substrate comprises a substrate layer,wherein the substrate layer comprises a substrate main body layer formed through ion implantation, an ion damage layer located on one side of the substrate main body layer and a substrate thin film layer arranged on the ion damage layer, and the substrate thin film layer is prepared into a functional layer through a semiconductor; and a stress importing layer and a supporting piece, wherein one of the stress importing layer and the supporting piece is positioned on one side, far away from the ion damage layer, of the functional layer, and the other one is positioned on one side, far away fromthe ion damage layer, of the substrate main body layer. According to the semiconductor substrate, the problem of substrate cracking caused by bending of a thin substrate is solved, the semiconductorsubstrate can be suitable for the thin substrate, and the separation success rate is improved.

The invention relates to a visual identity-based high-reliability method and device for automatically separating cells. The method comprises the following steps: centrifuging a centrifugal tube filledwith raw blood; acquiring a picture of the layering situation in the centrifugal tube after centrifuging treatment; identifying liquid levels of different layers according to the picture; and performing liquid separation and suction on the layers in the centrifugal tube according to the identified liquid levels. According to the method, after the centrifugal tube filled with raw blood is centrifuged by adopting a centrifugal machine, the picture of the layering situation in the centrifugal tube is acquired by adopting a visual identity structure, the picture is subjected to interface identification to acquire the liquid level of each layer, and each layer in the centrifugal tube can be subjected to liquid separation treatment with liquid separation equipment according to the liquid levels, so that the cells can be automatically separated and overall identified in terms of vision, and cell separation has high a success rate and high reliability.

The invention provides an isolation and culture method of dental pulp mesenchymal stem cells. The method comprises the following steps of: S1, taking out a dental sample, clamping and cracking the dental sample, then taking out dental pulp tissues in the dental sample, chopping the dental pulp tissues, and immersing the chopped dental pulp tissues in a culture medium for later use; S2, putting sterilized glass beads into a container, and soaking the glass beads in a complete culture medium for later use; S3, shallowly burying the dental pulp tissues prepared in the step S1 into the glass beadsprepared in the step S2, and performing culturing for 1-3 hours to obtain a glass bead and dental pulp tissue complex; and S4, carrying out subculture on the glass bead and dental pulp tissue complexprepared in the step S3, and collecting the glass bead and dental pulp tissue complex by trypsin to obtain the target dental pulp mesenchymal stem cells. According to the isolation and culture methodof the dental pulp mesenchymal stem cells provided by the invention, the technical problem that large-scale production of the dental pulp mesenchymal stem cells cannot be carried out in related technologies is solved.

The invention discloses a haemophilus parasuis culture medium and a preparation method thereof. The haemophilus parasuis culture medium comprises the following components: a basic culture medium, coenzyme I, horse serum and defibrated sheep blood. According to the method, coenzyme I, defibrated sheep blood and horse serum are added into a basic culture medium, so that the problem that staphylococcus aureus must be inoculated when haemophilus parasuis grows on a blood agar culture medium is solved, bacterial colonies are purer, and the probability of infectious microbe contamination in bacterial purification is effectively reduced; moreover, the culture medium provided by the invention has more sufficient nutrition, is more suitable for the growth characteristics of haemophilus parasuis, and is more stable in growth, larger in bacterial colony and longer in preservation time than a chocolate culture medium, and the cultured viable bacteria can be preserved for 30 days at the temperature of 2-8 DEG C. The haemophilus parasuis culture medium provided by the invention can be used for culturing, identifying and separating haemophilus parasuis, and effectively improves the separation success rate of the haemophilus parasuis.

The invention provides a single cell separator, application thereof in a single cell separation process and a monoclonal cell preparation method, and relates to the technical field of single cell separation. The single cell separator comprises a liquid transfer needle and a liquid suction device, wherein the liquid transfer needle tightly communicates with the liquid suction device without leakage; and the liquid transfer needle comprises an intravenous injection needle which is bent at an angle of 75 to 90 degrees at a position 0.5 to 1 cm away from a needle opening. By using the single cellseparator provided by the invention, single cells can be quickly separated and extracted from cell sap, and the problems of complicated operation of a limited dilution method and high equipment requirement of a flow cytometer method in an existing single cell separation method are effectively relieved.

The invention discloses a separation method of an epixylous medicinal fungus. The separation method comprises the step of germinating a medicinal fungus sporophore under a closed condition for mycelium culture; in the separation method, the fresh sporophore is used as a natural substrate, which is most applicable to mycelium germination, for separation and purification culture, the success rate for separating the epixylous fungus is significantly improved, and is as high as above 85%; by the separation method, a variety of medicinal fungi with very high application values are successfully separated, such as phellinus igniarius, trametesrobiniophila, tectochrysin, lucid ganoderma, phaeoporus obliquus and the like; a new way is provided for culturing fungi with high medicinal values.

The invention discloses a separation method of tobacco root black rot bacteria. The method includes the steps: acquiring rhizosphere soil of tobacco plants with tobacco root black rot symptoms; dryingand grinding the acquired rhizosphere soil; adhering the grinded rhizosphere soil onto carrot slices; placing the carrot slices adhered to the soil into a culture vessel to culture the carrot slicesfor 2-4 days; flushing the carrot slices by the aid of sterile water with 50 micro-liters / milliliters of penicillin and 50 micro-liters / milliliters streptomycin sulfate; continuing to culture the flushed carrot slices for 2-4 days; selecting mildew layers with tobacco root black rot typical symptoms on the carrot slices; purifying, culturing and storing the mildew layers. Growth of the tobacco root black rot bacteria is induced to restrain other bacteria from growing by the aid of different effects of components of carrot slices on the tobacco root black rot bacteria and the other fungi, and the separation method solves the problem that the other fungi easily covers the tobacco root black rot bacteria. The surfaces of treated carrot slices can rapidly grow hyphae, typical conidiophore stalks, conidiophores and chlamydospores, and the success rate reaches 96% or more.

The invention discloses a feeder for a flushable separator. One end of a discharge pipe is connected with a hopper, and the other end extends outward. Extending to the discharge pipe; the driving mechanism drives the feeding screw to rotate; the top of the hopper is open and covered with a filter, and a grate is set in the middle, and the mesh of the grate is smaller than the mesh of the filter; Install the water nozzle to spray water towards the grate; there is also a water delivery pipe on the side wall of the hopper, one end is connected to the bucket of the hopper through the water pump, and the other end is connected to the water nozzle; the helical blade of the feeding screw is provided with multiple sets of metal brushes along the circumference. One end of the upper bristle is fixedly connected to the helical blade, and the other end extends outward. The structure is simple and the operation is convenient. Before the concrete mortar enters the sand and gravel separator, the sand adhered to the stone can be washed away, so as to improve the sand and gravel separation effect, save washing water and avoid waste of water resources.

The invention discloses an automatic green splitting machine. The left side of a workbench is provided with a first gear arranged higher and a second gear arranged lower, and the first gear is meshedwith the second gear; a driving part is arranged behind the workbench, the driving part comprises a first roller arranged higher and a second roller arranged lower, the first roller is coaxially fixedto a first roller rotating shaft in a sleeving mode, and the second roller is coaxially fixed to a second roller rotating shaft in a sleeving mode; the first roller rotating shaft and the second roller rotating shaft vertically correspond to each other and are parallel to each other, and the first roller rotating shaft and the second roller rotating shaft extend horizontally from left to right; agap between the first roller and the second roller forms a channel for bamboo strips to pass through, and the upper surface of the workbench is flush with the channel; an output shaft of a motor on the right side of the workbench is coaxially connected with the right end of the second roller rotating shaft; and the second gear is fixedly arranged at the left end of the second roller rotating shaft, wherein the first gear is fixedly arranged at the left end of the first roller rotating shaft, and a blade is arranged behind the driving part.

A method for isolating and cultivating porcine epidemic diarrhea virus belongs to the technical field of veterinary biological products. It includes collecting intestinal contents and feces from clinically suspected PED sick piglets, adding physiological saline respectively, homogenizing the intestinal contents, vibrating and mixing the fecal samples, adding virus treatment solution, mixing evenly, standing, Centrifuge, take the supernatant, first filter through a microporous membrane, and then filter through a microporous membrane to obtain the PEDV virus stock solution; take Vero cells that have been cultured for 24 hours and grow well on the wall, discard the cell culture medium, and PEDV The virus stock solution and the maintenance solution were inoculated into the cell bottles, and the cell bottles were cultured in the incubator, and the cell lesions were observed every day, and the cell culture solution with obvious lesions were collected and stored at -20°C. The separation and cultivation method proposed by the invention has low cost and short separation period, and is beneficial to timely diagnosis and research of porcine epidemic diarrhea virus disease.