Visual identity-based high-reliability method and device for automatically separating cells

Abstract

The invention relates to a visual identity-based high-reliability method and device for automatically separating cells. The method comprises the following steps: centrifuging a centrifugal tube filledwith raw blood; acquiring a picture of the layering situation in the centrifugal tube after centrifuging treatment; identifying liquid levels of different layers according to the picture; and performing liquid separation and suction on the layers in the centrifugal tube according to the identified liquid levels. According to the method, after the centrifugal tube filled with raw blood is centrifuged by adopting a centrifugal machine, the picture of the layering situation in the centrifugal tube is acquired by adopting a visual identity structure, the picture is subjected to interface identification to acquire the liquid level of each layer, and each layer in the centrifugal tube can be subjected to liquid separation treatment with liquid separation equipment according to the liquid levels, so that the cells can be automatically separated and overall identified in terms of vision, and cell separation has high a success rate and high reliability.

Description

technical field [0001] The present invention relates to a cell separation method, in particular to a high-reliability automatic cell separation method and device based on visual recognition. Background technique [0002] Cell separation techniques are used to separate specific cells of interest from primary blood. The commonly used cell separation technology is the cell separation technology by centrifugal separation. [0003] The traditional centrifugal separation relies on manual separation, and the stability and consistency of the operation are poor. According to the physical condition of the patient, the height of the original blood after centrifugation varies widely, and some layers (such as the buffy coat) are very thin and difficult to achieve. Identify the presence or absence of cells, which in turn prevents complete cell isolation, resulting in cell isolation failure. [0004] Therefore, it is necessary to design a new cell separation method to realize the automat...

AI Technical Summary

Problems solved by technology

[0003] Traditional centrifugation relies on manual separation, and the stability and consistency of the operation are poor. Depending on the patient's physical condition, the height of the centrifuged layers of the original blood varies widely. Some layers (such as the buffy coat) are very thin and difficult to separate. Identifies the presence or absence of cells, which in turn prevents complete cell isolation, resulting in cell isolation failure

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