Preparation method of wild Cordyceps militaris strain

Abstract

The invention discloses a preparation method of wild Cordyceps militaris strains, which is to select the internal tissue at the junction of wild Cordyceps militaris sclerotia and fruiting bodies, separate and purify a culture medium with a specific formula, and use secondary coverage of the mother species for purification and rejuvenation to obtain Purified liquid stock of wild Cordyceps militaris, and then inoculated the purified liquid stock of wild Cordyceps militaris into tussah silkworm diapause pupae for culture, and selected whole stiff pupae cultured for 12 days (with high DPPH antioxidant activity) for cryopreservation. Not only is the operation simple, but also the separation success rate is high and the storage period can reach 30 months, which solves various problems existing in the prior art due to the low separation success rate and short storage period.

Description

technical field [0001] The invention belongs to a preparation method of wild edible and medicinal fungi strains, in particular to a preparation method of wild Cordyceps militaris strains which can effectively improve the separation success rate and prolong the preservation period. Background technique [0002] Cordyceps militaris belongs to the same genus and different species from Cordyceps sinensis in terms of taxonomic status. It belongs to Ascomycota, Ergotia, Ergotaceae, and Cordyceps, and is the type species of Cordyceps. Cordyceps militaris is rich in various active ingredients such as cordycepin, cordycepin and superoxide dismutase. It has the functions of inhibiting the growth of harmful bacteria in the human body, anti-cancer, antioxidant, improving immunity and lowering blood sugar. For this reason, it has been used as a substitute for Cordyceps sinensis. Products, used in health drugs and food. At present, commercially available Cordyceps militaris is mainly pre...

AI Technical Summary

Problems solved by technology

However, the existing wild species of Cordyceps militaris all use the middle and upper part of the fruiting body as the separation material. Although this part is highly active, the pollution rate is high, so that the mycelium of Cordyceps militaris loses its growth advantage compared with the mycelium of other fungi, and cannot be effectively purified. Separation success rate is low

In addition, the storage period of the existing strain preservation method can only reach several months. Even if the glycerol mycelia freezing (-80°C) preservation method is adopted, the preservation period does not exceed 18 months. The probability of bacterial contamination is high and easy to mutate

Images