Single cell separator, application thereof in single cell separation process and monoclonal cell preparation method
A single-cell separation and single-cell technology, applied in the field of single-cell separation, can solve the problems of complicated operation of limited dilution method and high requirements for flow cytometer method equipment, save experimental time, avoid cell counting and repeated dilution, and improve preparation. The effect of efficiency
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[0046] According to one aspect of the present invention, a method for preparing monoclonal cells, the method comprises the following steps:
[0047] Provide cell liquid containing suspended cells, then use the above-mentioned single cell separator to absorb single cells in the cell liquid under a microscope, and then culture the single cells to obtain monoclonal cells;
[0048] Or, provide cell liquid containing adherent cells, then use the above-mentioned single cell separator to absorb single cells in the cell liquid under a microscope, and then culture the single cells to obtain monoclonal cells.
[0049] The method for preparing monoclonal cells provided by the present invention, the method firstly provides cell liquid containing adherent cells or suspension cells, then under a microscope, uses the above-mentioned single cell separator to absorb the single cells in the cell liquid, and then the single cells Cultured to obtain monoclonal cells. The above method realizes th...
Embodiment 1
[0057] figure 1 Schematic diagram of the structure of the single cell separator provided in Example 1; wherein: liquid suction device 1, that is, a 20ul pipette; pipette needle 2, that is, a syringe needle that is folded at about 90° at a distance of about 0.8 cm from the needle mouth.
[0058] like figure 1 Shown, a kind of single-cell separator, the preparation method of described single-cell separator, comprises the following steps:
[0059] First, take a syringe needle, cut off the tip of the needle with scissors, and grind it flat; then use a hemostat to fold the needle at a distance of about 0.8 cm from the needle mouth to about 90°; then, fold the needle at about 90° The tail of the syringe needle was connected to a 20ul pipette to prepare a single cell isolation device.
Embodiment 2
[0061] A kind of single cell separator, the preparation method of described single cell separator, comprises the following steps:
[0062] First, take a syringe needle, cut off the tip of the needle with scissors, and grind it flat; then use a hemostatic forceps to fold the needle at about 0.5cm from the needle mouth to about 75°; then, fold the needle at about 75° The tail of the syringe needle was connected to a 20ul pipette to prepare a single cell isolation device.
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