Mycoplasma hyopneumoniae selective separation medium and preparation method thereof

Abstract

The invention belongs to the technical field of veterinary biology, and relates to a mycoplasma hyopneumoniae selective separation medium and a preparation method thereof. The mycoplasma hyopneumoniae selective separation medium comprises the main components of lactoalbumin hydrolysate, yeast extract powder, MEM (minimum essential medium), Hank 's solution, 0.1% phenol red solution, healthy horse serum, penicillin, deionized water, and specific growth inhibition serum. The mycoplasma hyopneumoniae selective separation medium can be used for the rapid separation and identification of mycoplasma hyopneumoniae, the preparation method is simple and feasible, experimental results are reliable, the separation time is shortened to 103h-122h, the time is shortened to 17%-23%, the separation success rate is as high as 75% above, and is more than 10 times of the separation success rate of mediums in the prior art.

Description

technical field [0001] The invention belongs to the technical field of veterinary biology, and relates to a selective separation medium for mycoplasma hyopneumoniae and a preparation method thereof. Background technique [0002] Mycoplasma hyopneumoniae is one of the important pathogens that cause contact chronic respiratory diseases in pigs. It exists widely all over the world and seriously endangers the healthy development of the pig industry. Cause other diseases and aggravate the symptoms. The inactivated vaccine against Mycoplasma hyopneumoniae can reduce the occurrence of panting disease, but the premise of a high-quality vaccine needs to be supported by a good vaccine strain and the prevalence of Mycoplasma hyopneumoniae in the region. The culture medium is a matrix in which various nutrients are artificially synthesized for the growth of microorganisms. Mycoplasma is a tiny prokaryotic organism without a cell wall. It can grow on artificial medium to form small col...

AI Technical Summary

Problems solved by technology

Mycoplasma is a tiny prokaryotic organism without a cell wall. It can grow on artificial medium to form small colonies, but the culture conditions are very harsh and the growth rate is slow. Hyopneumoniae often exists in pigs at the same time, and its growth rate is higher than that of Mycoplasma hyopneumoniae, so that the isolation and identification of Mycoplasma hyopneumoniae often causes contamination, which leads to the failure of Mycoplasma hyopneumoniae isolation, which makes it difficult for the research on the prevention and treatment of swine asthma

In the actual production process, for piglets with typical symptoms of Mycoplasma hyopneumoniae, the isolation rate of Mycoplasma hyopneumoniae from diseased materials is extremely low, even due to the contamination of pathogenic microorganisms such as Mycoplasma hyopneumoniae, the isolation rate is as low as 1-5%, and a new method is urgently needed Experimental Protocol to Solve the Isolation Problem of Mycoplasma Hyopneumoniae

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