Mycoplaa hyopneumoniae selective isolation medium and preparation method thereof

Abstract

Belonging to the field of veterinary biological products, the invention discloses a mycoplaa hyopneumoniae selective isolation medium and a preparation method thereof. The mycoplaa hyopneumoniae selective isolation medium comprises the following components: brain heart infusion, PPLO broth, 10*Hank's solution, yeast extracted liquid, lactalbumin hydrolysate, an amino acid composition, glucose, penicillin, nystatin, 0.1% phenol red, inactivated pig serum, specific growth inhibiting serum and deionized water. The mycoplaa hyopneumoniae selective isolation medium provided by the invention has thecharacteristics of short isolation time, high isolation success rate, viable bacteria titer and isolation sensitivity far higher than those of the medium in the prior art, and is suitable for rapid growth of mycoplaa hyopneumoniae.

Description

technical field [0001] The invention relates to the field of veterinary biological products, in particular to a selective separation medium for mycoplasma hyopneumoniae and a preparation method thereof. Background technique [0002] Mycoplasma swine pneumonia is a very common respiratory disease in pig farms. It is prevalent all over the world. It is commonly known as swine asthma. It is caused by Mycoplasma hyopneumoniae (Mhp). The pathogen mainly resides in the trachea, bronchi and bronchioles in pigs On the cilia of pigs, it seriously damages the physical defense mechanism of pig respiratory tract - defense mucosa, which increases the susceptibility of pigs to other respiratory pathogens, so it often causes co-infection of multiple pathogens, causing porcine respiratory syndrome (PRDC) or other diseases , Seriously endanger the development of pig industry. [0003] Mycoplasma hyopneumoniae is a facultative anaerobic bacterium with extremely demanding growth requirements ...

AI Technical Summary

Problems solved by technology

Although Mycoplasma hyopneumoniae can use the nutrients in the culture medium to synthesize the substances needed for its own growth and reproduction, due to the small molecular weight of its genome, the amount of information it carries is not much, and its biosynthetic ability is limited. Therefore, it needs to ingest cholesterol, amino acids, Fatty acids, nucleic acid precursors, vitamins and other substances, which make the medium composition of mycoplasma more complicated, and some special nutrients must be added

The growth rate of Mycoplasma hyopneumoniae in the medium is slow, and the titer of viable bacteria is at 10 7 -10 8 About CCU / ml, the titer of viable bacteria is low, not ideal

[0004] Mycoplasma is a tiny prokaryotic organism without a cell wall. It can grow on an artificial medium to form small colonies, but the culture conditions are very harsh and the growth rate is slow. Mycoplasma hyorhinophylla and Mycoplasma hyopneumoniae often exist in pigs at the same time, and Its growth rate is higher than that of Mycoplasma hyopneumoniae, so that the isolation and identification of Mycoplasma hyopneumoniae often causes pollution, which leads to the failure of Mycoplasma hyopneumoniae isolation, which makes it difficult for the research on the prevention and treatment of swine asthma

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