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A method for isolating woody medicinal fungi

A technology of fungi and crude drugs, applied in the field of microorganisms, can solve the problems of slow growth of woody fungi, low strain rate, contamination, etc., and achieve the effects of separation and purification, high success rate, and wide application range

Inactive Publication Date: 2017-12-26
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current isolation method is to directly put the fruiting body of the woody fungus into the culture medium of the petri dish, but because the woody fungus grows slowly, the probability of being contaminated is very high, and the rate of successful isolation of the target strain is extremely low (about 15%)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Preparation of culture medium

[0042] 1. Prepare materials according to the formula in Table 1

[0043] Table 1 Formula of wort culture medium of the present invention

[0044] ingredient

Content (g / L)

ingredient

Content (g / L)

Malt extract

20.0

KH 2 PO 4

3.0

Agar powder

18.0

Streptomycin

2.0

glucose

10.0g

penicillin

2.0

[0045] The formula of wort culture medium: malt extract powder, agar powder, glucose, KH 2 PO 4 , Streptomycin, penicillin, sterile water.

[0046] 2. Preparation method

[0047] Weigh malt extract powder, agar powder, glucose, KH according to the amount of the prepared medium 2 PO 4 , Dissolve in the corresponding volume of sterile water, stir evenly, add dilute HCl (concentration 0.5-1mol / L), adjust the pH of the medium to 5.0±0.2; then autoclave at 121°C for 30 min, then wait After the temperature of the culture medium is lowered to below 50°C, the amount of penicillin and streptomycin are added, and the wort culture medium is prepared ...

Embodiment 2

[0056] Example 2: Ganoderma separation:

[0057] 1. Preliminary identification of wood raw medicinal fungi

[0058] In August 2010, in Taishan Scenic Area, Tai'an City, Shandong Province, the fruiting bodies of wild Ganoderma lingzhi (Ganoderma lingzhi BJFC-C1265) from the rotted roots of oak trees were cut with a knife, and placed in a sterile airtight bag, stored at low temperature for later use; collected Ganoderma lucidum Fruiting body: Ganoderma lucidum fruiting body is one-year-old, with mesophytic or lateral stipe; the cap is flat and cap shape, cork cork when fresh, and becomes cork when dried. The extension can reach 16cm, the width can reach 12cm, and the base is thick. Up to 2.6cm; the upper surface of the cap is light yellowish brown when young, with a lacquer-like luster, when mature, the color is yellow-brown to purple-brown, with concentric rings, usually covered by brown spore powder; the edges are dull or sharp, after drying Often rolled inward; the surface of the...

Embodiment 3

[0072] Example 3: Isolation of Populus chinensis (i.e. Cytoporus waningii)

[0073] 1. Preliminary identification of wood raw medicinal fungi

[0074] In August 2007, in Changbai Mountain, Jilin Province, the fruiting bodies of wild poplar (Inonotusvaninii BJFC-C0301) from fallen broad-leaved trees were cut with a knife and placed in a sterile airtight bag, stored at low temperature for later use; the fruiting bodies of poplar: basidiomycetes were collected in the wild , Flat to stalkless lid type, odorless and tasteless when fresh; the surface of the cap is reddish brown to gray-black with inconspicuous ring bands; the edge is bright yellow, which turns blood red in KOH reagent; the surface of the orifice is chestnut brown, with refractive index Response: The sterile edge is obvious, bright yellow, up to 1mm wide, the edge of the tube mouth is thin, whole or torn; the fungus flesh is bright yellow to dark brown, hard wood, with concentric rings, up to 3cm thick, sometimes with one...

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PUM

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Abstract

The invention discloses a separation method of an epixylous medicinal fungus. The separation method comprises the step of germinating a medicinal fungus sporophore under a closed condition for mycelium culture; in the separation method, the fresh sporophore is used as a natural substrate, which is most applicable to mycelium germination, for separation and purification culture, the success rate for separating the epixylous fungus is significantly improved, and is as high as above 85%; by the separation method, a variety of medicinal fungi with very high application values are successfully separated, such as phellinus igniarius, trametesrobiniophila, tectochrysin, lucid ganoderma, phaeoporus obliquus and the like; a new way is provided for culturing fungi with high medicinal values.

Description

Technical field [0001] The invention relates to a method for separating large fungi, in particular to a method for separating woody medicinal fungi, and belongs to the field of microorganisms. Background technique [0002] Wood fungus refers to a type of fungus that grows on wood, such as all kinds of standing wood, dead wood, fallen wood, fallen branches, felling, logs, mineral wood, sleepers, bridges and other building materials and various wood products. , It can degrade lignin, cellulose or hemicellulose that make up plant cell walls in wood. Wood fungi are an important group of higher fungi. As a component of forest ecology, they secrete various biological enzymes to decompose cellulose, hemicellulose and lignin in wood into nutrients that can be used by other organisms. It is the main driving force for decomposing cellulose and lignin, the original component of wood, and plays a key role in degradation and reduction in the material circulation and energy flow of the forest...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12Q1/68
CPCC12N1/14C12Q1/6895C12Q2600/166
Inventor 戴玉成员瑗吴芳司静边禄森
Owner BEIJING FORESTRY UNIVERSITY
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