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Method for fast separating microbes in Puer ripe tea

A separation method and technology of microorganisms, applied in the direction of biochemical equipment and methods, microorganisms, microorganisms, etc., can solve problems such as no public reports, and achieve high separation success rate and strong pertinence

Inactive Publication Date: 2012-04-25
YUNNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Through document retrieval, do not see the public report identical with the present invention

Method used

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Embodiment Construction

[0020] The instruments and equipment used in the implementation of this method are those commonly used in microbial research.

[0021] The method includes the selection of culture medium, the determination of culture conditions, and the confirmation of microbes producing cyclothecia (commonly known as "golden flower"). details as follows:

[0022] 1. Preparation of culture medium

[0023] a Separation and purification medium: 1000 mL of 20% potato juice; 10 g of yeast extract; 140 g of glucose; 20 g of agar;

[0024] b. Culture medium for producing ocystosis: add 50g of tea leaves, add 30mL of water, pack into a 500mL conical flask, sterilize at 121.3°C for 15 minutes and set aside.

[0025] 2. Plate separation and culture conditions

[0026] Cultivation temperature: 25° C.; cultivation time: 7 days (colonies change from gray-green to yellow); humidity is controlled above 70%, and a relatively anoxic environment is created by wrapping the plate.

[0027] 3. Tea sample sepa...

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Abstract

The invention relates to a method for fast separating microbes in Puer ripe tea, in particular to a method for fast separating cleistothecium (commonly referred to as golden flowers) generating microbes in the Puer ripe tea, which belongs to the technical field of the separation culture of beneficial microbes fermented by the Puer ripe tea. According to the analysis on the golden flower occurrence environment condition, a separation culture medium is determined by an orthogonal optimization experimental method, and then, the basic culture condition is determined after the further comparison perfection and the comparison study on the culture condition, so the bacterial colony of cleistothecium generating bacteria on a flat plate is fast expanded, the bacterial colony is large, the aerial hypha growth is good, the asexual stage is shortened, the generation of yellow ascocarp cleistothecium (commonly referring to the golden flowers) of sexual multiplication structures is promoted, and the goals and requirements of fast separation and culture multiplication are reached. The method comprises the steps of culture medium preparation, flat plate separation culture condition determination, tea sample separation and microbe determination. The method has the characteristics of high speed, simplicity, high pertinence and high separation success rate.

Description

Technical field: [0001] The invention relates to a rapid separation method for microorganisms in cooked Pu'er tea, in particular to a rapid separation method for microorganisms in the yellow ascus-close cystic shell (commonly known as "Golden Flower") in cooked Pu'er tea, which belongs to beneficial microorganisms for fermentation of Pu'er tea The field of isolation and culture technology. Background technique: [0002] Yunnan is the hometown of Pu'er tea. As a famous traditional tea in history, Pu'er tea has become a unique and attractive health drink favored by consumers at home and abroad. The effect of fat. The unique health care function of Pu'er tea has been recognized by people at home and abroad. [0003] The unique processing technology and technology of cooked Pu-erh tea use a variety of beneficial microorganisms to participate in it and play a key role, which ultimately affects the quality and efficacy of Pu-erh tea. Its types and functions are not fully unders...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00
Inventor 程立忠张理珉
Owner YUNNAN UNIV
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