Separation method of tobacco root black rot bacteria
A black rot fungus and isolation method technology, applied in the field of germ isolation, can solve the problems of complicated operation process, easy to be covered by other fungi, slow growth rate, etc., and achieve the effect of safe test environment, saving test cost, less consumables and reagents
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Embodiment 1
[0029] A method for isolating tobacco root black rot fungus, the operation steps are:
[0030] Step 1: Collection of test samples Select the diseased plants with typical symptoms of tobacco root black rot and pull them out, gently peel off the soil above 2 mm from the root surface with a scalpel, shake off the rest of the soil and collect it as a rhizosphere soil sample, do not shake The fallen soil adhered to the roots is gently brushed off with a small brush, air-dried indoors, put into paper bags, numbered, and stored for later use.
[0031] Step 2: Induction of carrot discs The rhizosphere soil samples were crushed in a mortar and poured into sterilized Petri dishes. Disinfect the surface of fresh carrots with 75% alcohol, cut them into discs about 5 mm thick with a sterilized knife, dip the ground samples evenly, and place them on a Petri dish covered with absorbent cotton soaked in distilled water and filter paper cultured in a constant temperature incubator at 25 ℃, ob...
Embodiment 2
[0036] A method for isolating tobacco root black rot fungus, the operation steps are:
[0037] Step 1: Collection of test samples Select the diseased plants with typical symptoms of tobacco root black rot and pull them out, gently peel off the soil above 2 mm from the root surface with a scalpel, shake off the rest of the soil and collect it as a rhizosphere soil sample, do not shake The fallen soil adhered to the roots is gently brushed off with a small brush, air-dried indoors, put into paper bags, numbered, and stored for later use.
[0038] Step 2: Induction of carrot discs The rhizosphere soil samples were crushed in a mortar and poured into sterilized Petri dishes. Disinfect the surface of fresh carrots with 75% alcohol, cut them into discs about 6 mm thick with a sterilized knife, dip the ground samples evenly, and place them on a Petri dish covered with absorbent cotton soaked in distilled water and filter paper cultured in a constant temperature incubator at 25 ℃, du...
Embodiment 3
[0043] A method for isolating tobacco root black rot fungus, the operation steps are:
[0044] Step 1: Collection of test samples Select the diseased plants with typical symptoms of tobacco root black rot and pull them out, gently peel off the soil above 2 mm from the root surface with a scalpel, shake off the rest of the soil and collect it as a rhizosphere soil sample, do not shake The fallen soil adhered to the roots is gently brushed off with a small brush, air-dried indoors, put into paper bags, numbered, and stored for later use.
[0045] Step 2: Induction of carrot discs The rhizosphere soil samples were crushed in a mortar and poured into sterilized Petri dishes. Disinfect the surface of fresh carrots with 75% alcohol, cut them into discs about 4mm thick with a sterilized knife, dip the ground samples evenly, and place them in a Petri dish covered with absorbent cotton soaked in distilled water and filter paper , cultured in a constant temperature incubator at 25 °C, ...
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